LOCUS       BBa_K228010             1930 bp    DNA     linear       01-Jun-2024
DEFINITION  LuxR-LuxP-GFP
ACCESSION   BBa_K228010
VERSION     BBa_K228010.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    64..65
                     /label=prefix
     misc_feature    237..237
                     /label=T
     misc_feature    555..555
                     /label=A
     CDS             64..813
                     /label=luxR
     misc_feature    64..819
                     /label=BBa_C0062
     misc_feature    820..844
                     /label=Help:Barcodes
     CDS             64..819
                     /label=BBa_C0062
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
     misc_feature    46..981
                     /label=BBa_I0462
     misc_feature    990..1009
                     /label=LuxR/HSL
     promoter        1009..1014
                     /label=-35
     promoter        1031..1036
                     /label=-10
     misc_feature    1042..1042
                     /label=start
     misc_feature    990..1044
                     /label=BBa_R0062
     promoter        990..1044
                     /label=BBa_R0062
     misc_feature    1..1044
                     /label=BBa_J09855
     RBS             1053..1067
                     /label=RBS-1\Strong
     RBS             1060..1064
                     /label=RBS
     misc_feature    1053..1067
                     /label=BBa_B0030
     RBS             1053..1067
                     /label=BBa_B0030
     CDS             1074..1793
                     /label=GFP protein
     CDS             1074..1793
                     /label=BBa_E0040
     stem_loop       1813..1856
                     /label=stem_loop
     misc_feature    1802..1881
                     /label=BBa_B0010
     terminator      1802..1881
                     /label=BBa_B0010
     stem_loop       1897..1916
                     /label=T7 TE
     misc_feature    1923..1923
                     /label=stop
     polyA_site      1917..1930
                     /label=polya
     misc_feature    1890..1930
                     /label=BBa_B0012
     terminator      1890..1930
                     /label=BBa_B0012
     misc_feature    1053..1930
                     /label=BBa_E0840
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
      121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
      181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
      241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
      301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
      361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
      421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
      481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
      541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
      601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
      661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
      721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
      781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
      841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa
     1021 aatggtttgt tatagtcgaa taaatactag agattaaaga ggagaaatac tagatgcgta
     1081 aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat ggtgatgtta
     1141 atgggcacaa attttctgtc agtggagagg gtgaaggtga tgcaacatac ggaaaactta
     1201 cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca cttgtcacta
     1261 ctttcggtta tggtgttcaa tgctttgcga gatacccaga tcatatgaaa cagcatgact
     1321 ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt ttcaaagatg
     1381 acgggaacta caagacacgt gctgaagtca agtttgaagg tgataccctt gttaatagaa
     1441 tcgagttaaa aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat
     1501 acaactataa ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag
     1561 ttaacttcaa aattagacac aacattgaag atggaagcgt tcaactagca gaccattatc
     1621 aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat tacctgtcca
     1681 cacaatctgc cctttcgaaa gatcccaacg aaaagagaga ccacatggtc cttcttgagt
     1741 ttgtaacagc tgctgggatt acacatggca tggatgaact atacaaataa taatactaga
     1801 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1861 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1921 tgcgtttata
//