LOCUS BBa_K228119 4372 bp DNA linear 10-Jun-2024 DEFINITION AND GATE LacP+RBS(B0030)+T7ptag+LuxP+SupD ACCESSION BBa_K228119 VERSION BBa_K228119.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 misc_feature 1053..1188 /label=BBa_K228001 stem_loop 1208..1251 /label=stem_loop misc_feature 1197..1276 /label=BBa_B0010 terminator 1197..1276 /label=BBa_B0010 stem_loop 1292..1311 /label=T7 TE misc_feature 1318..1318 /label=stop polyA_site 1312..1325 /label=polya misc_feature 1285..1325 /label=BBa_B0012 terminator 1285..1325 /label=BBa_B0012 misc_feature 1053..1325 /label=BBa_K228100 misc_feature 1..1325 /label=BBa_K228011 CDS 1334..1421 /label=end of LacI coding region (inactive) misc_feature 1334..1533 /label=BBa_R0010 misc_feature 1422..1459 /label=CAP binding site promoter 1470..1475 /label=-35 promoter 1494..1499 /label=-10 misc_feature 1499..1533 /label=LacI binding site misc_feature 1506..1506 /label=start promoter 1334..1533 /label=BBa_R0010 RBS 1542..1556 /label=RBS-1\Strong RBS 1549..1553 /label=RBS misc_feature 1542..1556 /label=BBa_B0030 RBS 1542..1556 /label=BBa_B0030 CDS 1563..4235 /label=cds CDS 1563..4235 /label=BBa_K228000 stem_loop 4255..4298 /label=stem_loop misc_feature 4244..4323 /label=BBa_B0010 terminator 4244..4323 /label=BBa_B0010 stem_loop 4339..4358 /label=T7 TE misc_feature 4365..4365 /label=stop polyA_site 4359..4372 /label=polya misc_feature 4332..4372 /label=BBa_B0012 terminator 4332..4372 /label=BBa_B0012 misc_feature 1334..4372 /label=BBa_K228101 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agcaattcgg agagatgccg gagcggctga 1081 acggaccggt ctctaaaacc ggagtagggg caactctacc gggggttcaa atccccctct 1141 ctccgccact acagatcctt agcgaaagct aaggattttt tttaagctta ctagagccag 1201 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt 1261 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt 1321 ttatatacta gagcaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc 1381 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg 1441 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg 1501 tgtggaattg tgagcggata acaatttcac acatactaga gattaaagag gagaaatact 1561 agatgaccat gattaccgtg cactagaata ccattaacat tgctaagaac gacttctctg 1621 acatcgaact ggctgctatc ccgttcaaca ctctggctga ccattacggt gagcgtttag 1681 ctcgcgaaca gttggccctt gagcatgagt cttacgagat gggtgaagca cgcttccgca 1741 agatgtttga gcgtcaactt aaagctggtg aggttgcgga taacgctgcc gccaagcctc 1801 tcatcactac cctactccct aagatgattg cacgcatcaa cgactggttt gaggaagtga 1861 aagctaagcg cggcaagcgc ccgacagcct tccagttcct gtaggaaatc aagccggaag 1921 ccgtagcgta catcaccatt aagaccactc tggcttgcct aaccagtgct gacaatacaa 1981 ccgttcaggc tgtagcaagc gcaatcggtc gggccattga ggacgaggct cgcttcggtc 2041 gtatccgtga ccttgaagct aagcacttca agaaaaacgt tgaggaacaa ctcaacaagc 2101 gcgtagggca cgtctacaag aaagcattta tgcaagttgt cgaggctgac atgctctcta 2161 agggtctact cggtggcgag gcgtggtctt cgtggcataa ggaagactct attcatgtag 2221 gagtacgctg catcgagatg ctcattgagt caaccggaat ggttagctta caccgccaaa 2281 atgctggcgt agtaggtcaa gactctgaga ctatcgaact cgcacctgaa tacgctgagg 2341 ctatcgcaac ccgtgcaggt gcgctggctg gcatctctcc gatgttccaa ccttgcgtag 2401 ttcctcctaa gccgtggact ggcattactg gtggtggcta ttgggctaac ggtcgtcgtc 2461 ctctggcgct ggtgcgtact cacagtaaga aagcactgat gcgctacgaa gacgtttaca 2521 tgcctgaggt gtacaaagcg attaacattg cgcaaaacac cgcatggaaa atcaacaaga 2581 aagtcctagc ggtcgccaac gtaatcacca agtggaagca ttgtccggtc gaggacatcc 2641 ctgcgattga gcgtgaagaa ctcccgatga aaccggaaga catcgacatg aatcctgagg 2701 ctctcaccgc gtggaaacgt gctgccgctg ctgtgtaccg caaggacaag gctcgcaagt 2761 ctcgccgtat cagccttgag ttcatgcttg agcaagccaa taagtttgct aaccataagg 2821 ccatctggtt cccttacaac atggactggc gcggtcgtgt ttacgctgtg tcaatgttca 2881 acccgcaagg taacgatatg accaaaggac tgcttacgct ggcgaaaggt aaaccaatcg 2941 gtaaggaagg ttactactgg ctgaaaatcc acggtgcaaa ctgtgcgggt gtcgataagg 3001 ttccgttccc tgagcgcatc aagttcattg aggaaaacca cgagaacatc atggcttgcg 3061 ctaagtctcc actggagaac acttggtggg ctgagcaaga ttctccgttc tgcttccttg 3121 cgttctgctt tgagtacgct ggggtacagc accacggcct gagctataac tgctcccttc 3181 cgctggcgtt tgacgggtct tgctctggca tccagcactt ctccgcgatg ctccgagatg 3241 aggtaggtgg tcgcgcggtt aacttgcttc ctagtgaaac cgttcaggac atctacggga 3301 ttgttgctaa gaaagtcaac gagattctac aagcagacgc aatcaatggg accgataacg 3361 aagtagttac cgtgaccgat gagaacactg gtgaaatctc tgagaaagtc aagctgggca 3421 ctaaggcact ggctggtcaa tggctggctt acggtgttac tcgcagtgtg actaagcgtt 3481 cagtcatgac gctggcttac gggtccaaag agttcggctt ccgtcaacaa gtgctggaag 3541 ataccattca gccagctatt gattccggca agggtctgat gttcactcag ccgaatcagg 3601 ctgctggata catggctaag ctgatttggg aatctgtgag cgtgacggtg gtagctgcgg 3661 ttgaagcaat gaactggctt aagtctgctg ctaagctgct ggctgctgag gtcaaagata 3721 agaagactgg agagattctt cgcaagcgtt gcgctgtgca ttgggtaact cctgatggtt 3781 tccctgtgtg gcaggaatac aagaagccta ttcagacgcg cttgaacctg atgttcctcg 3841 gtcagttccg cttacagcct accattaaca ccaacaaaga tagcgagatt gatgcacaca 3901 aacaggagtc tggtatcgct cctaactttg tacacagcca agacggtagc caccttcgta 3961 agactgtagt gtgggcacac gagaagtacg gaatcgaatc ttttgcactg attcacgact 4021 ccttcggtac cattccggct gacgctgcga acctgttcaa agcagtgcgc gaaactatgg 4081 ttgacacata tgagtcttgt gatgtactgg ctgatttcta cgaccagttc gctgaccagt 4141 tgcacgagtc tcaattggac aaaatgccag cacttccggc taaaggtaac ttgaacctcc 4201 gtgacatctt agagtcggac ttcgcgttcg cataatacta gagccaggca tcaaataaaa 4261 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 4321 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //