LOCUS       BBa_K228201             2830 bp    DNA     linear       02-Jun-2024
DEFINITION  RBS->T7ptag->Terminator
ACCESSION   BBa_K228201
VERSION     BBa_K228201.1  
FEATURES             Location/Qualifiers
     misc_feature    7..10
                     /label=conserved
     RBS             1..14
                     /label=BBa_B0031
     CDS             21..2693
                     /label=cds
     CDS             21..2693
                     /label=BBa_K228000
     stem_loop       2713..2756
                     /label=stem_loop
     misc_feature    2702..2781
                     /label=BBa_B0010
     terminator      2702..2781
                     /label=BBa_B0010
     stem_loop       2797..2816
                     /label=T7 TE
     misc_feature    2823..2823
                     /label=stop
     polyA_site      2817..2830
                     /label=polya
     misc_feature    2790..2830
                     /label=BBa_B0012
     terminator      2790..2830
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aacctactag atgaccatga ttaccgtgca ctagaatacc attaacattg
       61 ctaagaacga cttctctgac atcgaactgg ctgctatccc gttcaacact ctggctgacc
      121 attacggtga gcgtttagct cgcgaacagt tggcccttga gcatgagtct tacgagatgg
      181 gtgaagcacg cttccgcaag atgtttgagc gtcaacttaa agctggtgag gttgcggata
      241 acgctgccgc caagcctctc atcactaccc tactccctaa gatgattgca cgcatcaacg
      301 actggtttga ggaagtgaaa gctaagcgcg gcaagcgccc gacagccttc cagttcctgt
      361 aggaaatcaa gccggaagcc gtagcgtaca tcaccattaa gaccactctg gcttgcctaa
      421 ccagtgctga caatacaacc gttcaggctg tagcaagcgc aatcggtcgg gccattgagg
      481 acgaggctcg cttcggtcgt atccgtgacc ttgaagctaa gcacttcaag aaaaacgttg
      541 aggaacaact caacaagcgc gtagggcacg tctacaagaa agcatttatg caagttgtcg
      601 aggctgacat gctctctaag ggtctactcg gtggcgaggc gtggtcttcg tggcataagg
      661 aagactctat tcatgtagga gtacgctgca tcgagatgct cattgagtca accggaatgg
      721 ttagcttaca ccgccaaaat gctggcgtag taggtcaaga ctctgagact atcgaactcg
      781 cacctgaata cgctgaggct atcgcaaccc gtgcaggtgc gctggctggc atctctccga
      841 tgttccaacc ttgcgtagtt cctcctaagc cgtggactgg cattactggt ggtggctatt
      901 gggctaacgg tcgtcgtcct ctggcgctgg tgcgtactca cagtaagaaa gcactgatgc
      961 gctacgaaga cgtttacatg cctgaggtgt acaaagcgat taacattgcg caaaacaccg
     1021 catggaaaat caacaagaaa gtcctagcgg tcgccaacgt aatcaccaag tggaagcatt
     1081 gtccggtcga ggacatccct gcgattgagc gtgaagaact cccgatgaaa ccggaagaca
     1141 tcgacatgaa tcctgaggct ctcaccgcgt ggaaacgtgc tgccgctgct gtgtaccgca
     1201 aggacaaggc tcgcaagtct cgccgtatca gccttgagtt catgcttgag caagccaata
     1261 agtttgctaa ccataaggcc atctggttcc cttacaacat ggactggcgc ggtcgtgttt
     1321 acgctgtgtc aatgttcaac ccgcaaggta acgatatgac caaaggactg cttacgctgg
     1381 cgaaaggtaa accaatcggt aaggaaggtt actactggct gaaaatccac ggtgcaaact
     1441 gtgcgggtgt cgataaggtt ccgttccctg agcgcatcaa gttcattgag gaaaaccacg
     1501 agaacatcat ggcttgcgct aagtctccac tggagaacac ttggtgggct gagcaagatt
     1561 ctccgttctg cttccttgcg ttctgctttg agtacgctgg ggtacagcac cacggcctga
     1621 gctataactg ctcccttccg ctggcgtttg acgggtcttg ctctggcatc cagcacttct
     1681 ccgcgatgct ccgagatgag gtaggtggtc gcgcggttaa cttgcttcct agtgaaaccg
     1741 ttcaggacat ctacgggatt gttgctaaga aagtcaacga gattctacaa gcagacgcaa
     1801 tcaatgggac cgataacgaa gtagttaccg tgaccgatga gaacactggt gaaatctctg
     1861 agaaagtcaa gctgggcact aaggcactgg ctggtcaatg gctggcttac ggtgttactc
     1921 gcagtgtgac taagcgttca gtcatgacgc tggcttacgg gtccaaagag ttcggcttcc
     1981 gtcaacaagt gctggaagat accattcagc cagctattga ttccggcaag ggtctgatgt
     2041 tcactcagcc gaatcaggct gctggataca tggctaagct gatttgggaa tctgtgagcg
     2101 tgacggtggt agctgcggtt gaagcaatga actggcttaa gtctgctgct aagctgctgg
     2161 ctgctgaggt caaagataag aagactggag agattcttcg caagcgttgc gctgtgcatt
     2221 gggtaactcc tgatggtttc cctgtgtggc aggaatacaa gaagcctatt cagacgcgct
     2281 tgaacctgat gttcctcggt cagttccgct tacagcctac cattaacacc aacaaagata
     2341 gcgagattga tgcacacaaa caggagtctg gtatcgctcc taactttgta cacagccaag
     2401 acggtagcca ccttcgtaag actgtagtgt gggcacacga gaagtacgga atcgaatctt
     2461 ttgcactgat tcacgactcc ttcggtacca ttccggctga cgctgcgaac ctgttcaaag
     2521 cagtgcgcga aactatggtt gacacatatg agtcttgtga tgtactggct gatttctacg
     2581 accagttcgc tgaccagttg cacgagtctc aattggacaa aatgccagca cttccggcta
     2641 aaggtaactt gaacctccgt gacatcttag agtcggactt cgcgttcgca taatactaga
     2701 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     2761 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     2821 tgcgtttata
//