LOCUS BBa_K228218 3883 bp DNA linear 01-Jun-2024 DEFINITION LuxR->rbs->T7ptag->Terminator ACCESSION BBa_K228218 VERSION BBa_K228218.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 RBS 1053..1067 /label=RBS-1\Strong RBS 1060..1064 /label=RBS misc_feature 1053..1067 /label=BBa_B0030 RBS 1053..1067 /label=BBa_B0030 CDS 1074..3746 /label=cds CDS 1074..3746 /label=BBa_K228000 stem_loop 3766..3809 /label=stem_loop misc_feature 3755..3834 /label=BBa_B0010 terminator 3755..3834 /label=BBa_B0010 stem_loop 3850..3869 /label=T7 TE misc_feature 3876..3876 /label=stop polyA_site 3870..3883 /label=polya misc_feature 3843..3883 /label=BBa_B0012 terminator 3843..3883 /label=BBa_B0012 misc_feature 1053..3883 /label=BBa_K228200 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agattaaaga ggagaaatac tagatgacca 1081 tgattaccgt gcactagaat accattaaca ttgctaagaa cgacttctct gacatcgaac 1141 tggctgctat cccgttcaac actctggctg accattacgg tgagcgttta gctcgcgaac 1201 agttggccct tgagcatgag tcttacgaga tgggtgaagc acgcttccgc aagatgtttg 1261 agcgtcaact taaagctggt gaggttgcgg ataacgctgc cgccaagcct ctcatcacta 1321 ccctactccc taagatgatt gcacgcatca acgactggtt tgaggaagtg aaagctaagc 1381 gcggcaagcg cccgacagcc ttccagttcc tgtaggaaat caagccggaa gccgtagcgt 1441 acatcaccat taagaccact ctggcttgcc taaccagtgc tgacaataca accgttcagg 1501 ctgtagcaag cgcaatcggt cgggccattg aggacgaggc tcgcttcggt cgtatccgtg 1561 accttgaagc taagcacttc aagaaaaacg ttgaggaaca actcaacaag cgcgtagggc 1621 acgtctacaa gaaagcattt atgcaagttg tcgaggctga catgctctct aagggtctac 1681 tcggtggcga ggcgtggtct tcgtggcata aggaagactc tattcatgta ggagtacgct 1741 gcatcgagat gctcattgag tcaaccggaa tggttagctt acaccgccaa aatgctggcg 1801 tagtaggtca agactctgag actatcgaac tcgcacctga atacgctgag gctatcgcaa 1861 cccgtgcagg tgcgctggct ggcatctctc cgatgttcca accttgcgta gttcctccta 1921 agccgtggac tggcattact ggtggtggct attgggctaa cggtcgtcgt cctctggcgc 1981 tggtgcgtac tcacagtaag aaagcactga tgcgctacga agacgtttac atgcctgagg 2041 tgtacaaagc gattaacatt gcgcaaaaca ccgcatggaa aatcaacaag aaagtcctag 2101 cggtcgccaa cgtaatcacc aagtggaagc attgtccggt cgaggacatc cctgcgattg 2161 agcgtgaaga actcccgatg aaaccggaag acatcgacat gaatcctgag gctctcaccg 2221 cgtggaaacg tgctgccgct gctgtgtacc gcaaggacaa ggctcgcaag tctcgccgta 2281 tcagccttga gttcatgctt gagcaagcca ataagtttgc taaccataag gccatctggt 2341 tcccttacaa catggactgg cgcggtcgtg tttacgctgt gtcaatgttc aacccgcaag 2401 gtaacgatat gaccaaagga ctgcttacgc tggcgaaagg taaaccaatc ggtaaggaag 2461 gttactactg gctgaaaatc cacggtgcaa actgtgcggg tgtcgataag gttccgttcc 2521 ctgagcgcat caagttcatt gaggaaaacc acgagaacat catggcttgc gctaagtctc 2581 cactggagaa cacttggtgg gctgagcaag attctccgtt ctgcttcctt gcgttctgct 2641 ttgagtacgc tggggtacag caccacggcc tgagctataa ctgctccctt ccgctggcgt 2701 ttgacgggtc ttgctctggc atccagcact tctccgcgat gctccgagat gaggtaggtg 2761 gtcgcgcggt taacttgctt cctagtgaaa ccgttcagga catctacggg attgttgcta 2821 agaaagtcaa cgagattcta caagcagacg caatcaatgg gaccgataac gaagtagtta 2881 ccgtgaccga tgagaacact ggtgaaatct ctgagaaagt caagctgggc actaaggcac 2941 tggctggtca atggctggct tacggtgtta ctcgcagtgt gactaagcgt tcagtcatga 3001 cgctggctta cgggtccaaa gagttcggct tccgtcaaca agtgctggaa gataccattc 3061 agccagctat tgattccggc aagggtctga tgttcactca gccgaatcag gctgctggat 3121 acatggctaa gctgatttgg gaatctgtga gcgtgacggt ggtagctgcg gttgaagcaa 3181 tgaactggct taagtctgct gctaagctgc tggctgctga ggtcaaagat aagaagactg 3241 gagagattct tcgcaagcgt tgcgctgtgc attgggtaac tcctgatggt ttccctgtgt 3301 ggcaggaata caagaagcct attcagacgc gcttgaacct gatgttcctc ggtcagttcc 3361 gcttacagcc taccattaac accaacaaag atagcgagat tgatgcacac aaacaggagt 3421 ctggtatcgc tcctaacttt gtacacagcc aagacggtag ccaccttcgt aagactgtag 3481 tgtgggcaca cgagaagtac ggaatcgaat cttttgcact gattcacgac tccttcggta 3541 ccattccggc tgacgctgcg aacctgttca aagcagtgcg cgaaactatg gttgacacat 3601 atgagtcttg tgatgtactg gctgatttct acgaccagtt cgctgaccag ttgcacgagt 3661 ctcaattgga caaaatgcca gcacttccgg ctaaaggtaa cttgaacctc cgtgacatct 3721 tagagtcgga cttcgcgttc gcataatact agagccaggc atcaaataaa acgaaaggct 3781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 3841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //