LOCUS BBa_K228221 3879 bp DNA linear 01-Jun-2024 DEFINITION LuxR->rbs->T7ptag->Terminator ACCESSION BBa_K228221 VERSION BBa_K228221.1 FEATURES Location/Qualifiers misc_feature 1..37 /label=P(Lac) IQ promoter 3..8 /label=-35 promoter 26..31 /label=-10 promoter 1..37 /label=BBa_I14032 misc_feature 50..53 /label=conserved RBS 46..57 /label=BBa_B0034 misc_feature 64..65 /label=prefix misc_feature 237..237 /label=T misc_feature 555..555 /label=A CDS 64..813 /label=luxR misc_feature 64..819 /label=BBa_C0062 misc_feature 820..844 /label=Help:Barcodes CDS 64..819 /label=BBa_C0062 stem_loop 864..907 /label=stem_loop misc_feature 853..932 /label=BBa_B0010 terminator 853..932 /label=BBa_B0010 stem_loop 948..967 /label=T7 TE misc_feature 974..974 /label=stop polyA_site 968..981 /label=polya misc_feature 941..981 /label=BBa_B0012 terminator 941..981 /label=BBa_B0012 misc_feature 46..981 /label=BBa_I0462 misc_feature 990..1009 /label=LuxR/HSL promoter 1009..1014 /label=-35 promoter 1031..1036 /label=-10 misc_feature 1042..1042 /label=start misc_feature 990..1044 /label=BBa_R0062 promoter 990..1044 /label=BBa_R0062 misc_feature 1..1044 /label=BBa_J09855 RBS 1053..1063 /label=RBS-4\Weaker RBS 1059..1062 /label=RBS misc_feature 1053..1063 /label=BBa_B0033 RBS 1053..1063 /label=BBa_B0033 CDS 1070..3742 /label=cds CDS 1070..3742 /label=BBa_K228000 stem_loop 3762..3805 /label=stem_loop misc_feature 3751..3830 /label=BBa_B0010 terminator 3751..3830 /label=BBa_B0010 stem_loop 3846..3865 /label=T7 TE misc_feature 3872..3872 /label=stop polyA_site 3866..3879 /label=polya misc_feature 3839..3879 /label=BBa_B0012 terminator 3839..3879 /label=BBa_B0012 misc_feature 1053..3879 /label=BBa_K228203 ORIGIN 1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac 61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt 121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa 181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta 241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat 301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac 361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc 421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca 481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca 541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca 601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc 661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc 721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta 781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga 841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa 1021 aatggtttgt tatagtcgaa taaatactag agtcacacag gactactaga tgaccatgat 1081 taccgtgcac tagaatacca ttaacattgc taagaacgac ttctctgaca tcgaactggc 1141 tgctatcccg ttcaacactc tggctgacca ttacggtgag cgtttagctc gcgaacagtt 1201 ggcccttgag catgagtctt acgagatggg tgaagcacgc ttccgcaaga tgtttgagcg 1261 tcaacttaaa gctggtgagg ttgcggataa cgctgccgcc aagcctctca tcactaccct 1321 actccctaag atgattgcac gcatcaacga ctggtttgag gaagtgaaag ctaagcgcgg 1381 caagcgcccg acagccttcc agttcctgta ggaaatcaag ccggaagccg tagcgtacat 1441 caccattaag accactctgg cttgcctaac cagtgctgac aatacaaccg ttcaggctgt 1501 agcaagcgca atcggtcggg ccattgagga cgaggctcgc ttcggtcgta tccgtgacct 1561 tgaagctaag cacttcaaga aaaacgttga ggaacaactc aacaagcgcg tagggcacgt 1621 ctacaagaaa gcatttatgc aagttgtcga ggctgacatg ctctctaagg gtctactcgg 1681 tggcgaggcg tggtcttcgt ggcataagga agactctatt catgtaggag tacgctgcat 1741 cgagatgctc attgagtcaa ccggaatggt tagcttacac cgccaaaatg ctggcgtagt 1801 aggtcaagac tctgagacta tcgaactcgc acctgaatac gctgaggcta tcgcaacccg 1861 tgcaggtgcg ctggctggca tctctccgat gttccaacct tgcgtagttc ctcctaagcc 1921 gtggactggc attactggtg gtggctattg ggctaacggt cgtcgtcctc tggcgctggt 1981 gcgtactcac agtaagaaag cactgatgcg ctacgaagac gtttacatgc ctgaggtgta 2041 caaagcgatt aacattgcgc aaaacaccgc atggaaaatc aacaagaaag tcctagcggt 2101 cgccaacgta atcaccaagt ggaagcattg tccggtcgag gacatccctg cgattgagcg 2161 tgaagaactc ccgatgaaac cggaagacat cgacatgaat cctgaggctc tcaccgcgtg 2221 gaaacgtgct gccgctgctg tgtaccgcaa ggacaaggct cgcaagtctc gccgtatcag 2281 ccttgagttc atgcttgagc aagccaataa gtttgctaac cataaggcca tctggttccc 2341 ttacaacatg gactggcgcg gtcgtgttta cgctgtgtca atgttcaacc cgcaaggtaa 2401 cgatatgacc aaaggactgc ttacgctggc gaaaggtaaa ccaatcggta aggaaggtta 2461 ctactggctg aaaatccacg gtgcaaactg tgcgggtgtc gataaggttc cgttccctga 2521 gcgcatcaag ttcattgagg aaaaccacga gaacatcatg gcttgcgcta agtctccact 2581 ggagaacact tggtgggctg agcaagattc tccgttctgc ttccttgcgt tctgctttga 2641 gtacgctggg gtacagcacc acggcctgag ctataactgc tcccttccgc tggcgtttga 2701 cgggtcttgc tctggcatcc agcacttctc cgcgatgctc cgagatgagg taggtggtcg 2761 cgcggttaac ttgcttccta gtgaaaccgt tcaggacatc tacgggattg ttgctaagaa 2821 agtcaacgag attctacaag cagacgcaat caatgggacc gataacgaag tagttaccgt 2881 gaccgatgag aacactggtg aaatctctga gaaagtcaag ctgggcacta aggcactggc 2941 tggtcaatgg ctggcttacg gtgttactcg cagtgtgact aagcgttcag tcatgacgct 3001 ggcttacggg tccaaagagt tcggcttccg tcaacaagtg ctggaagata ccattcagcc 3061 agctattgat tccggcaagg gtctgatgtt cactcagccg aatcaggctg ctggatacat 3121 ggctaagctg atttgggaat ctgtgagcgt gacggtggta gctgcggttg aagcaatgaa 3181 ctggcttaag tctgctgcta agctgctggc tgctgaggtc aaagataaga agactggaga 3241 gattcttcgc aagcgttgcg ctgtgcattg ggtaactcct gatggtttcc ctgtgtggca 3301 ggaatacaag aagcctattc agacgcgctt gaacctgatg ttcctcggtc agttccgctt 3361 acagcctacc attaacacca acaaagatag cgagattgat gcacacaaac aggagtctgg 3421 tatcgctcct aactttgtac acagccaaga cggtagccac cttcgtaaga ctgtagtgtg 3481 ggcacacgag aagtacggaa tcgaatcttt tgcactgatt cacgactcct tcggtaccat 3541 tccggctgac gctgcgaacc tgttcaaagc agtgcgcgaa actatggttg acacatatga 3601 gtcttgtgat gtactggctg atttctacga ccagttcgct gaccagttgc acgagtctca 3661 attggacaaa atgccagcac ttccggctaa aggtaacttg aacctccgtg acatcttaga 3721 gtcggacttc gcgttcgcat aatactagag ccaggcatca aataaaacga aaggctcagt 3781 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 3841 acactggctc accttcgggt gggcctttct gcgtttata //