LOCUS       BBa_K228819             2448 bp    DNA     linear       28-May-2024
DEFINITION  Pcat+RBS(B0034)+lacI(C0012)+term(B0015)+lacP(R0010)+GFP
ACCESSION   BBa_K228819
VERSION     BBa_K228819.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     misc_feature    1154..1192
                     /label=LVA
     CDS             65..1192
                     /label=lacI-LVA
     misc_feature    65..1192
                     /label=BBa_C0012
     misc_feature    1193..1217
                     /label=Help:Barcodes
     CDS             65..1192
                     /label=BBa_C0012
     stem_loop       1237..1280
                     /label=stem_loop
     misc_feature    1226..1305
                     /label=BBa_B0010
     terminator      1226..1305
                     /label=BBa_B0010
     stem_loop       1321..1340
                     /label=T7 TE
     misc_feature    1347..1347
                     /label=stop
     polyA_site      1341..1354
                     /label=polya
     misc_feature    1314..1354
                     /label=BBa_B0012
     terminator      1314..1354
                     /label=BBa_B0012
     terminator      1226..1354
                     /label=BBa_B0015
     CDS             1363..1450
                     /label=end of LacI coding region (inactive)
     misc_feature    1363..1562
                     /label=BBa_R0010
     misc_feature    1451..1488
                     /label=CAP binding site
     promoter        1499..1504
                     /label=-35
     promoter        1523..1528
                     /label=-10
     misc_feature    1528..1562
                     /label=LacI binding site
     misc_feature    1535..1535
                     /label=start
     promoter        1363..1562
                     /label=BBa_R0010
     RBS             1571..1585
                     /label=RBS-1\Strong
     RBS             1578..1582
                     /label=RBS
     misc_feature    1571..1585
                     /label=BBa_B0030
     RBS             1571..1585
                     /label=BBa_B0030
     CDS             1592..2311
                     /label=GFP protein
     CDS             1592..2311
                     /label=BBa_E0040
     stem_loop       2331..2374
                     /label=stem_loop
     misc_feature    2320..2399
                     /label=BBa_B0010
     terminator      2320..2399
                     /label=BBa_B0010
     stem_loop       2415..2434
                     /label=T7 TE
     misc_feature    2441..2441
                     /label=stop
     polyA_site      2435..2448
                     /label=polya
     misc_feature    2408..2448
                     /label=BBa_B0012
     terminator      2408..2448
                     /label=BBa_B0012
     misc_feature    1571..2448
                     /label=BBa_E0840
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatggtg aatgtgaaac cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc
      121 ttatcagacc gtttcccgcg tggtgaacca ggccagccac gtttctgcga aaacgcggga
      181 aaaagtggaa gcggcgatgg cggagctgaa ttacattccc aaccgcgtgg cacaacaact
      241 ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc agtctggccc tgcacgcgcc
      301 gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt
      361 gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc
      421 gcaacgcgtc agtgggctga tcattaacta tccgctggat gaccaggatg ccattgctgt
      481 ggaagctgcc tgcactaatg ttccggcgtt atttcttgat gtctctgacc agacacccat
      541 caacagtatt attttctccc atgaagacgg tacgcgactg ggcgtggagc atctggtcgc
      601 attgggtcac cagcaaatcg cgctgttagc gggcccatta agttctgtct cggcgcgtct
      661 gcgtctggct ggctggcata aatatctcac tcgcaatcaa attcagccga tagcggaacg
      721 ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg
      781 catcgttccc actgcgatgc tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc
      841 cattaccgag tccgggctgc gcgttggtgc ggatatctcg gtagtgggat acgacgatac
      901 cgaagacagc tcatgttata tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct
      961 ggggcaaacc agcgtggacc gcttgctgca actctctcag ggccaggcgg tgaagggcaa
     1021 tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac
     1081 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     1141 ggaaagcggg caggctgcaa acgacgaaaa ctacgcttta gtagcttaat aactctgata
     1201 gtgctagtgt agatctctac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1261 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1321 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agcaatacgc aaaccgcctc
     1381 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     1441 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     1501 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     1561 catactagag attaaagagg agaaatacta gatgcgtaaa ggagaagaac ttttcactgg
     1621 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
     1681 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
     1741 tggaaaacta cctgttccat ggccaacact tgtcactact ttcggttatg gtgttcaatg
     1801 ctttgcgaga tacccagatc atatgaaaca gcatgacttt ttcaagagtg ccatgcccga
     1861 aggttatgta caggaaagaa ctatattttt caaagatgac gggaactaca agacacgtgc
     1921 tgaagtcaag tttgaaggtg atacccttgt taatagaatc gagttaaaag gtattgattt
     1981 taaagaagat ggaaacattc ttggacacaa attggaatac aactataact cacacaatgt
     2041 atacatcatg gcagacaaac aaaagaatgg aatcaaagtt aacttcaaaa ttagacacaa
     2101 cattgaagat ggaagcgttc aactagcaga ccattatcaa caaaatactc caattggcga
     2161 tggccctgtc cttttaccag acaaccatta cctgtccaca caatctgccc tttcgaaaga
     2221 tcccaacgaa aagagagacc acatggtcct tcttgagttt gtaacagctg ctgggattac
     2281 acatggcatg gatgaactat acaaataata atactagagc caggcatcaa ataaaacgaa
     2341 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2401 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//