LOCUS       BBa_K228820             1834 bp    DNA     linear       28-May-2024
DEFINITION  Pcat+RBS(B0034)+tetR(C0040)+term(B0015)+tetP(R0040)+GFP
ACCESSION   BBa_K228820
VERSION     BBa_K228820.1  
FEATURES             Location/Qualifiers
     promoter        4..9
                     /label=-35
     promoter        27..32
                     /label=-10
     misc_feature    1..38
                     /label=P(Cat)
     promoter        1..38
                     /label=BBa_I14033
     misc_feature    51..54
                     /label=conserved
     RBS             47..58
                     /label=BBa_B0034
     CDS             68..684
                     /label=tetR
     misc_feature    685..718
                     /label=SsrA
     misc_feature    725..749
                     /label=Help:Barcodes
     CDS             65..749
                     /label=BBa_C0040
     stem_loop       769..812
                     /label=stem_loop
     misc_feature    758..837
                     /label=BBa_B0010
     terminator      758..837
                     /label=BBa_B0010
     stem_loop       853..872
                     /label=T7 TE
     misc_feature    879..879
                     /label=stop
     polyA_site      873..886
                     /label=polya
     misc_feature    846..886
                     /label=BBa_B0012
     terminator      846..886
                     /label=BBa_B0012
     terminator      758..886
                     /label=BBa_B0015
     misc_feature    895..913
                     /label=TetR 1
     misc_feature    895..948
                     /label=BBa_R0040
     promoter        914..919
                     /label=-35
     misc_feature    920..938
                     /label=TetR 2
     promoter        937..942
                     /label=-10
     promoter        895..948
                     /label=BBa_R0040
     RBS             957..971
                     /label=RBS-1\Strong
     RBS             964..968
                     /label=RBS
     misc_feature    957..971
                     /label=BBa_B0030
     RBS             957..971
                     /label=BBa_B0030
     CDS             978..1697
                     /label=GFP protein
     CDS             978..1697
                     /label=BBa_E0040
     stem_loop       1717..1760
                     /label=stem_loop
     misc_feature    1706..1785
                     /label=BBa_B0010
     terminator      1706..1785
                     /label=BBa_B0010
     stem_loop       1801..1820
                     /label=T7 TE
     misc_feature    1827..1827
                     /label=stop
     polyA_site      1821..1834
                     /label=polya
     misc_feature    1794..1834
                     /label=BBa_B0012
     terminator      1794..1834
                     /label=BBa_B0012
     misc_feature    957..1834
                     /label=BBa_E0840
ORIGIN
        1 ggcacgtaag aggttccaac tttcaccata atgaaacata ctagagaaag aggagaaata
       61 ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
      121 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
      181 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
      241 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
      301 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
      361 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
      421 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
      481 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
      541 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
      601 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
      661 acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta
      721 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag
      781 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      841 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtcccta
      901 tcagtgatag agattgacat ccctatcagt gatagagata ctgagcacta ctagagatta
      961 aagaggagaa atactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc
     1021 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
     1081 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
     1141 ttccatggcc aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc
     1201 cagatcatat gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     1261 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
     1321 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
     1381 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
     1441 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
     1501 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     1561 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
     1621 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
     1681 aactatacaa ataataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa
     1741 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact
     1801 ggctcacctt cgggtgggcc tttctgcgtt tata
//