LOCUS BBa_K228827 4607 bp DNA linear 23-May-2024 DEFINITION AND GATE lacP+SupD+AraC+RBS(B0030)+T7ptag ACCESSION BBa_K228827 VERSION BBa_K228827.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 209..344 /label=BBa_K228001 stem_loop 364..407 /label=stem_loop misc_feature 353..432 /label=BBa_B0010 terminator 353..432 /label=BBa_B0010 stem_loop 448..467 /label=T7 TE misc_feature 474..474 /label=stop polyA_site 468..481 /label=polya misc_feature 441..481 /label=BBa_B0012 terminator 441..481 /label=BBa_B0012 misc_feature 1..481 /label=BBa_K228822 stem_loop 1513..1556 /label=stem_loop misc_feature 1502..1581 /label=BBa_B0010 terminator 1502..1581 /label=BBa_B0010 stem_loop 1597..1616 /label=T7 TE misc_feature 1623..1623 /label=stop polyA_site 1617..1630 /label=polya misc_feature 1590..1630 /label=BBa_B0012 terminator 1590..1630 /label=BBa_B0012 promoter 1639..1768 /label=BBa_I13453 misc_feature 490..527 /label=P(Cat) promoter 493..498 /label=-35 promoter 516..521 /label=-10 misc_feature 490..527 /label=BBa_I14033 misc_feature 536..547 /label=BBa_B0034 misc_feature 540..543 /label=conserved misc_feature 554..1468 /label=BBa_C0080 CDS 554..1429 /label=araC misc_feature 1430..1462 /label=LVA misc_feature 1469..1493 /label=barcode misc_feature 1502..1581 /label=BBa_B0010 stem_loop 1513..1556 /label=stem_loop misc_feature 1590..1630 /label=BBa_B0012 stem_loop 1597..1616 /label=T7 TE polyA_site 1617..1630 /label=polya misc_feature 1623..1623 /label=stop misc_feature 536..1630 /label=BBa_K228006 misc_feature 1639..1768 /label=BBa_I13453 misc_feature 490..1630 /label=BBa_K228008 promoter 1639..1768 /label=BBa_I13453 promoter 490..1768 /label=BBa_K228009 RBS 1777..1791 /label=RBS-1\Strong RBS 1784..1788 /label=RBS misc_feature 1777..1791 /label=BBa_B0030 RBS 1777..1791 /label=BBa_B0030 CDS 1798..4470 /label=cds CDS 1798..4470 /label=BBa_K228000 stem_loop 4490..4533 /label=stem_loop misc_feature 4479..4558 /label=BBa_B0010 terminator 4479..4558 /label=BBa_B0010 stem_loop 4574..4593 /label=T7 TE misc_feature 4600..4600 /label=stop polyA_site 4594..4607 /label=polya misc_feature 4567..4607 /label=BBa_B0012 terminator 4567..4607 /label=BBa_B0012 terminator 4479..4607 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagca attcggagag atgccggagc ggctgaacgg 241 accggtctct aaaaccggag taggggcaac tctaccgggg gttcaaatcc ccctctctcc 301 gccactacag atccttagcg aaagctaagg atttttttta agcttactag agccaggcat 361 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 421 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 481 atactagagt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga 541 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc 601 gttttatttg atgcctggct ctagtagtga tctacactag cactatcagt gttattaagc 661 tactaaagcg tagttttcgt cgtttgcagc tgacaacttg acggctacat cattcacttt 721 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatactcg 781 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg 841 ggtagtgctc aaaagcagct tcgcctggct gatgcgttgg tcctcgcgcc agcttaagac 901 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca ggcaaacatg 961 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca 1021 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg 1081 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc 1141 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc 1201 cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta 1261 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc 1321 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc 1381 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt 1441 cattcccagc ggtcggtcga taaaaaaatc gagataacca ttggcctcaa tcggcgttaa 1501 acccgccacc agatgggcgt taaacgagta tcccggcagc aggggatcat tttgcgcttc 1561 agccatctag tatttctcct ctttctctag tatgtttcat tatggtgaaa gttggaacct 1621 cttacgtgcc tactagagac attgattatt tgcacggcgt cacactttgc tatgccatag 1681 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg 1741 tttctccata ccgttttttt gggctagcta ctagagatta aagaggagaa atactagatg 1801 accatgatta ccgtgcacta gaataccatt aacattgcta agaacgactt ctctgacatc 1861 gaactggctg ctatcccgtt caacactctg gctgaccatt acggtgagcg tttagctcgc 1921 gaacagttgg cccttgagca tgagtcttac gagatgggtg aagcacgctt ccgcaagatg 1981 tttgagcgtc aacttaaagc tggtgaggtt gcggataacg ctgccgccaa gcctctcatc 2041 actaccctac tccctaagat gattgcacgc atcaacgact ggtttgagga agtgaaagct 2101 aagcgcggca agcgcccgac agccttccag ttcctgtagg aaatcaagcc ggaagccgta 2161 gcgtacatca ccattaagac cactctggct tgcctaacca gtgctgacaa tacaaccgtt 2221 caggctgtag caagcgcaat cggtcgggcc attgaggacg aggctcgctt cggtcgtatc 2281 cgtgaccttg aagctaagca cttcaagaaa aacgttgagg aacaactcaa caagcgcgta 2341 gggcacgtct acaagaaagc atttatgcaa gttgtcgagg ctgacatgct ctctaagggt 2401 ctactcggtg gcgaggcgtg gtcttcgtgg cataaggaag actctattca tgtaggagta 2461 cgctgcatcg agatgctcat tgagtcaacc ggaatggtta gcttacaccg ccaaaatgct 2521 ggcgtagtag gtcaagactc tgagactatc gaactcgcac ctgaatacgc tgaggctatc 2581 gcaacccgtg caggtgcgct ggctggcatc tctccgatgt tccaaccttg cgtagttcct 2641 cctaagccgt ggactggcat tactggtggt ggctattggg ctaacggtcg tcgtcctctg 2701 gcgctggtgc gtactcacag taagaaagca ctgatgcgct acgaagacgt ttacatgcct 2761 gaggtgtaca aagcgattaa cattgcgcaa aacaccgcat ggaaaatcaa caagaaagtc 2821 ctagcggtcg ccaacgtaat caccaagtgg aagcattgtc cggtcgagga catccctgcg 2881 attgagcgtg aagaactccc gatgaaaccg gaagacatcg acatgaatcc tgaggctctc 2941 accgcgtgga aacgtgctgc cgctgctgtg taccgcaagg acaaggctcg caagtctcgc 3001 cgtatcagcc ttgagttcat gcttgagcaa gccaataagt ttgctaacca taaggccatc 3061 tggttccctt acaacatgga ctggcgcggt cgtgtttacg ctgtgtcaat gttcaacccg 3121 caaggtaacg atatgaccaa aggactgctt acgctggcga aaggtaaacc aatcggtaag 3181 gaaggttact actggctgaa aatccacggt gcaaactgtg cgggtgtcga taaggttccg 3241 ttccctgagc gcatcaagtt cattgaggaa aaccacgaga acatcatggc ttgcgctaag 3301 tctccactgg agaacacttg gtgggctgag caagattctc cgttctgctt ccttgcgttc 3361 tgctttgagt acgctggggt acagcaccac ggcctgagct ataactgctc ccttccgctg 3421 gcgtttgacg ggtcttgctc tggcatccag cacttctccg cgatgctccg agatgaggta 3481 ggtggtcgcg cggttaactt gcttcctagt gaaaccgttc aggacatcta cgggattgtt 3541 gctaagaaag tcaacgagat tctacaagca gacgcaatca atgggaccga taacgaagta 3601 gttaccgtga ccgatgagaa cactggtgaa atctctgaga aagtcaagct gggcactaag 3661 gcactggctg gtcaatggct ggcttacggt gttactcgca gtgtgactaa gcgttcagtc 3721 atgacgctgg cttacgggtc caaagagttc ggcttccgtc aacaagtgct ggaagatacc 3781 attcagccag ctattgattc cggcaagggt ctgatgttca ctcagccgaa tcaggctgct 3841 ggatacatgg ctaagctgat ttgggaatct gtgagcgtga cggtggtagc tgcggttgaa 3901 gcaatgaact ggcttaagtc tgctgctaag ctgctggctg ctgaggtcaa agataagaag 3961 actggagaga ttcttcgcaa gcgttgcgct gtgcattggg taactcctga tggtttccct 4021 gtgtggcagg aatacaagaa gcctattcag acgcgcttga acctgatgtt cctcggtcag 4081 ttccgcttac agcctaccat taacaccaac aaagatagcg agattgatgc acacaaacag 4141 gagtctggta tcgctcctaa ctttgtacac agccaagacg gtagccacct tcgtaagact 4201 gtagtgtggg cacacgagaa gtacggaatc gaatcttttg cactgattca cgactccttc 4261 ggtaccattc cggctgacgc tgcgaacctg ttcaaagcag tgcgcgaaac tatggttgac 4321 acatatgagt cttgtgatgt actggctgat ttctacgacc agttcgctga ccagttgcac 4381 gagtctcaat tggacaaaat gccagcactt ccggctaaag gtaacttgaa cctccgtgac 4441 atcttagagt cggacttcgc gttcgcataa tactagagcc aggcatcaaa taaaacgaaa 4501 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta 4561 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //