LOCUS BBa_K228830 4606 bp DNA linear 23-May-2024 DEFINITION AND GATE lacP+SupD+AraC+RBS(B0031)+T7ptag ACCESSION BBa_K228830 VERSION BBa_K228830.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 209..344 /label=BBa_K228001 stem_loop 364..407 /label=stem_loop misc_feature 353..432 /label=BBa_B0010 terminator 353..432 /label=BBa_B0010 stem_loop 448..467 /label=T7 TE misc_feature 474..474 /label=stop polyA_site 468..481 /label=polya misc_feature 441..481 /label=BBa_B0012 terminator 441..481 /label=BBa_B0012 misc_feature 1..481 /label=BBa_K228822 stem_loop 1513..1556 /label=stem_loop misc_feature 1502..1581 /label=BBa_B0010 terminator 1502..1581 /label=BBa_B0010 stem_loop 1597..1616 /label=T7 TE misc_feature 1623..1623 /label=stop polyA_site 1617..1630 /label=polya misc_feature 1590..1630 /label=BBa_B0012 terminator 1590..1630 /label=BBa_B0012 promoter 1639..1768 /label=BBa_I13453 misc_feature 490..527 /label=P(Cat) promoter 493..498 /label=-35 promoter 516..521 /label=-10 misc_feature 490..527 /label=BBa_I14033 misc_feature 536..547 /label=BBa_B0034 misc_feature 540..543 /label=conserved misc_feature 554..1468 /label=BBa_C0080 CDS 554..1429 /label=araC misc_feature 1430..1462 /label=LVA misc_feature 1469..1493 /label=barcode misc_feature 1502..1581 /label=BBa_B0010 stem_loop 1513..1556 /label=stem_loop misc_feature 1590..1630 /label=BBa_B0012 stem_loop 1597..1616 /label=T7 TE polyA_site 1617..1630 /label=polya misc_feature 1623..1623 /label=stop misc_feature 536..1630 /label=BBa_K228006 misc_feature 1639..1768 /label=BBa_I13453 misc_feature 490..1630 /label=BBa_K228008 promoter 1639..1768 /label=BBa_I13453 promoter 490..1768 /label=BBa_K228009 misc_feature 1783..1786 /label=conserved RBS 1777..1790 /label=BBa_B0031 CDS 1797..4469 /label=cds CDS 1797..4469 /label=BBa_K228000 stem_loop 4489..4532 /label=stem_loop misc_feature 4478..4557 /label=BBa_B0010 terminator 4478..4557 /label=BBa_B0010 stem_loop 4573..4592 /label=T7 TE misc_feature 4599..4599 /label=stop polyA_site 4593..4606 /label=polya misc_feature 4566..4606 /label=BBa_B0012 terminator 4566..4606 /label=BBa_B0012 terminator 4478..4606 /label=BBa_B0015 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagca attcggagag atgccggagc ggctgaacgg 241 accggtctct aaaaccggag taggggcaac tctaccgggg gttcaaatcc ccctctctcc 301 gccactacag atccttagcg aaagctaagg atttttttta agcttactag agccaggcat 361 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 421 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 481 atactagagt ataaacgcag aaaggcccac ccgaaggtga gccagtgtga ctctagtaga 541 gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac tgagcctttc 601 gttttatttg atgcctggct ctagtagtga tctacactag cactatcagt gttattaagc 661 tactaaagcg tagttttcgt cgtttgcagc tgacaacttg acggctacat cattcacttt 721 ttcttcacaa ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatactcg 781 cgagaaatag agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg 841 ggtagtgctc aaaagcagct tcgcctggct gatgcgttgg tcctcgcgcc agcttaagac 901 gctaatccct aactgctggc ggaaaagatg tgacagacgc gacggcgaca ggcaaacatg 961 ctgtgcgacg ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca 1021 agcctcgcgt acccgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg 1081 ccgcagtaac aattgctcaa gcagatttat cgccagcagc tccgaatagc gcccttcccc 1141 ttgcccggcg ttaatgattt gcccaaacag gtcgctgaaa tgcggctggt gcgcttcatc 1201 cgggcgaaag aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta 1261 ggcgcgcgga cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacgacc 1321 gtagtgatga atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc 1381 tcgtccctga tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt 1441 cattcccagc ggtcggtcga taaaaaaatc gagataacca ttggcctcaa tcggcgttaa 1501 acccgccacc agatgggcgt taaacgagta tcccggcagc aggggatcat tttgcgcttc 1561 agccatctag tatttctcct ctttctctag tatgtttcat tatggtgaaa gttggaacct 1621 cttacgtgcc tactagagac attgattatt tgcacggcgt cacactttgc tatgccatag 1681 catttttatc cataagatta gcggatccta cctgacgctt tttatcgcaa ctctctactg 1741 tttctccata ccgttttttt gggctagcta ctagagtcac acaggaaacc tactagatga 1801 ccatgattac cgtgcactag aataccatta acattgctaa gaacgacttc tctgacatcg 1861 aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt ttagctcgcg 1921 aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc cgcaagatgt 1981 ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag cctctcatca 2041 ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa gtgaaagcta 2101 agcgcggcaa gcgcccgaca gccttccagt tcctgtagga aatcaagccg gaagccgtag 2161 cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat acaaccgttc 2221 aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc ggtcgtatcc 2281 gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac aagcgcgtag 2341 ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc tctaagggtc 2401 tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat gtaggagtac 2461 gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc caaaatgctg 2521 gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct gaggctatcg 2581 caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc gtagttcctc 2641 ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt cgtcctctgg 2701 cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt tacatgcctg 2761 aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac aagaaagtcc 2821 tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac atccctgcga 2881 ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct gaggctctca 2941 ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc aagtctcgcc 3001 gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat aaggccatct 3061 ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg ttcaacccgc 3121 aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca atcggtaagg 3181 aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat aaggttccgt 3241 tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct tgcgctaagt 3301 ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc cttgcgttct 3361 gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc cttccgctgg 3421 cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga gatgaggtag 3481 gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac gggattgttg 3541 ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat aacgaagtag 3601 ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg ggcactaagg 3661 cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag cgttcagtca 3721 tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg gaagatacca 3781 ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat caggctgctg 3841 gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct gcggttgaag 3901 caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa gataagaaga 3961 ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat ggtttccctg 4021 tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc ctcggtcagt 4081 tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca cacaaacagg 4141 agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt cgtaagactg 4201 tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac gactccttcg 4261 gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact atggttgaca 4321 catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac cagttgcacg 4381 agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac ctccgtgaca 4441 tcttagagtc ggacttcgcg ttcgcataat actagagcca ggcatcaaat aaaacgaaag 4501 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 4561 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //