LOCUS BBa_K228859 4376 bp DNA linear 10-Jun-2024 DEFINITION AND GATE Sal+SupD+LacP+RBS(B0033)+T7ptag ACCESSION BBa_K228859 VERSION BBa_K228859.1 FEATURES Location/Qualifiers CDS 1..903 /label=NahR promoter 904..993 /label=Pr promoter promoter 982..1050 /label=pSal promoter 1..1052 /label=BBa_K228004 misc_feature 1061..1196 /label=BBa_K228001 stem_loop 1216..1259 /label=stem_loop misc_feature 1205..1284 /label=BBa_B0010 terminator 1205..1284 /label=BBa_B0010 stem_loop 1300..1319 /label=T7 TE misc_feature 1326..1326 /label=stop polyA_site 1320..1333 /label=polya misc_feature 1293..1333 /label=BBa_B0012 terminator 1293..1333 /label=BBa_B0012 CDS 1342..1429 /label=end of LacI coding region (inactive) misc_feature 1342..1541 /label=BBa_R0010 misc_feature 1430..1467 /label=CAP binding site promoter 1478..1483 /label=-35 promoter 1502..1507 /label=-10 misc_feature 1507..1541 /label=LacI binding site misc_feature 1514..1514 /label=start promoter 1342..1541 /label=BBa_R0010 RBS 1550..1560 /label=RBS-4\Weaker RBS 1556..1559 /label=RBS misc_feature 1550..1560 /label=BBa_B0033 RBS 1550..1560 /label=BBa_B0033 CDS 1567..4239 /label=cds CDS 1567..4239 /label=BBa_K228000 stem_loop 4259..4302 /label=stem_loop misc_feature 4248..4327 /label=BBa_B0010 terminator 4248..4327 /label=BBa_B0010 stem_loop 4343..4362 /label=T7 TE misc_feature 4369..4369 /label=stop polyA_site 4363..4376 /label=polya misc_feature 4336..4376 /label=BBa_B0012 terminator 4336..4376 /label=BBa_B0012 ORIGIN 1 tcaatccgta aacaggtcaa acatcagttg ccgcaaccaa atattggcta ggtccttgtg 61 gtacttcgca tgccagaaca tgttgatggc tatttcaggc aagacgactg ggtgcggcaa 121 ggcgcttagg ccgaagggct ccacgcagca gtcggctaaa cgtatcggca cagtggcgag 181 cagatcggtg cgctggagga tgtggccaac ggcggcgaag tgcggcactt ccagacggat 241 gtcgcgccgg atgccgaccc gtgtcatgta cgtgtccacc tcgccgtggc cggtgccagc 301 ggcgatgaca cgcacgtggc cgtaggaaca gaagcgctcc agagtcaggg gttcgcgggt 361 gactggatgg tccttgcgac ataggcacac gtagtgattc tggagcagcc ggcgctgaaa 421 gaagccagtt tgcagattgg gaagcaggcc cacggccaag tccacggttc cgttctgcaa 481 ggcctgcatc aggctcatcg aactgtcgcg caccgtactg atcacgcaat tgggggcctg 541 gtgagccagc acatccatca gccgcggcat gaagtagatc tcgccaatgt cggtcatggc 601 cagggtgaag gtacgctcgc tggtcagcgg atcgaagctt tcatggtgct gtagggcgtt 661 gcgcagtgcg tgcatggccg aagtgacggg ctcggccaga tgcgcggcat agggtgtggg 721 ttccattccc tgatgtgtgc gcacgaagag tgggtcctgt agcgaggtgc gcaggcgttt 781 cagcgcattg ctcacggcag gctgggtcag gcccaggttc tccgcagtga tagagacgcg 841 tctgtcgacc agcaactggt tgaacaccac cagcaggttt aaatccaggt cacgcagttc 901 catggggcct cgcttgggtt attgctggtg cccggccggg cgcaatattc atgttgatga 961 tttattatat atcgagtggt gtatttatca atattgtttg ctccgttatc gttattaaca 1021 agtcatcaat aaagccatca cgagtaccat agtactagag caattcggag agatgccgga 1081 gcggctgaac ggaccggtct ctaaaaccgg agtaggggca actctaccgg gggttcaaat 1141 ccccctctct ccgccactac agatccttag cgaaagctaa ggattttttt taagcttact 1201 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 1261 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 1321 ttctgcgttt atatactaga gcaatacgca aaccgcctct ccccgcgcgt tggccgattc 1381 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 1441 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 1501 gtatgttgtg tggaattgtg agcggataac aatttcacac atactagagt cacacaggac 1561 tactagatga ccatgattac cgtgcactag aataccatta acattgctaa gaacgacttc 1621 tctgacatcg aactggctgc tatcccgttc aacactctgg ctgaccatta cggtgagcgt 1681 ttagctcgcg aacagttggc ccttgagcat gagtcttacg agatgggtga agcacgcttc 1741 cgcaagatgt ttgagcgtca acttaaagct ggtgaggttg cggataacgc tgccgccaag 1801 cctctcatca ctaccctact ccctaagatg attgcacgca tcaacgactg gtttgaggaa 1861 gtgaaagcta agcgcggcaa gcgcccgaca gccttccagt tcctgtagga aatcaagccg 1921 gaagccgtag cgtacatcac cattaagacc actctggctt gcctaaccag tgctgacaat 1981 acaaccgttc aggctgtagc aagcgcaatc ggtcgggcca ttgaggacga ggctcgcttc 2041 ggtcgtatcc gtgaccttga agctaagcac ttcaagaaaa acgttgagga acaactcaac 2101 aagcgcgtag ggcacgtcta caagaaagca tttatgcaag ttgtcgaggc tgacatgctc 2161 tctaagggtc tactcggtgg cgaggcgtgg tcttcgtggc ataaggaaga ctctattcat 2221 gtaggagtac gctgcatcga gatgctcatt gagtcaaccg gaatggttag cttacaccgc 2281 caaaatgctg gcgtagtagg tcaagactct gagactatcg aactcgcacc tgaatacgct 2341 gaggctatcg caacccgtgc aggtgcgctg gctggcatct ctccgatgtt ccaaccttgc 2401 gtagttcctc ctaagccgtg gactggcatt actggtggtg gctattgggc taacggtcgt 2461 cgtcctctgg cgctggtgcg tactcacagt aagaaagcac tgatgcgcta cgaagacgtt 2521 tacatgcctg aggtgtacaa agcgattaac attgcgcaaa acaccgcatg gaaaatcaac 2581 aagaaagtcc tagcggtcgc caacgtaatc accaagtgga agcattgtcc ggtcgaggac 2641 atccctgcga ttgagcgtga agaactcccg atgaaaccgg aagacatcga catgaatcct 2701 gaggctctca ccgcgtggaa acgtgctgcc gctgctgtgt accgcaagga caaggctcgc 2761 aagtctcgcc gtatcagcct tgagttcatg cttgagcaag ccaataagtt tgctaaccat 2821 aaggccatct ggttccctta caacatggac tggcgcggtc gtgtttacgc tgtgtcaatg 2881 ttcaacccgc aaggtaacga tatgaccaaa ggactgctta cgctggcgaa aggtaaacca 2941 atcggtaagg aaggttacta ctggctgaaa atccacggtg caaactgtgc gggtgtcgat 3001 aaggttccgt tccctgagcg catcaagttc attgaggaaa accacgagaa catcatggct 3061 tgcgctaagt ctccactgga gaacacttgg tgggctgagc aagattctcc gttctgcttc 3121 cttgcgttct gctttgagta cgctggggta cagcaccacg gcctgagcta taactgctcc 3181 cttccgctgg cgtttgacgg gtcttgctct ggcatccagc acttctccgc gatgctccga 3241 gatgaggtag gtggtcgcgc ggttaacttg cttcctagtg aaaccgttca ggacatctac 3301 gggattgttg ctaagaaagt caacgagatt ctacaagcag acgcaatcaa tgggaccgat 3361 aacgaagtag ttaccgtgac cgatgagaac actggtgaaa tctctgagaa agtcaagctg 3421 ggcactaagg cactggctgg tcaatggctg gcttacggtg ttactcgcag tgtgactaag 3481 cgttcagtca tgacgctggc ttacgggtcc aaagagttcg gcttccgtca acaagtgctg 3541 gaagatacca ttcagccagc tattgattcc ggcaagggtc tgatgttcac tcagccgaat 3601 caggctgctg gatacatggc taagctgatt tgggaatctg tgagcgtgac ggtggtagct 3661 gcggttgaag caatgaactg gcttaagtct gctgctaagc tgctggctgc tgaggtcaaa 3721 gataagaaga ctggagagat tcttcgcaag cgttgcgctg tgcattgggt aactcctgat 3781 ggtttccctg tgtggcagga atacaagaag cctattcaga cgcgcttgaa cctgatgttc 3841 ctcggtcagt tccgcttaca gcctaccatt aacaccaaca aagatagcga gattgatgca 3901 cacaaacagg agtctggtat cgctcctaac tttgtacaca gccaagacgg tagccacctt 3961 cgtaagactg tagtgtgggc acacgagaag tacggaatcg aatcttttgc actgattcac 4021 gactccttcg gtaccattcc ggctgacgct gcgaacctgt tcaaagcagt gcgcgaaact 4081 atggttgaca catatgagtc ttgtgatgta ctggctgatt tctacgacca gttcgctgac 4141 cagttgcacg agtctcaatt ggacaaaatg ccagcacttc cggctaaagg taacttgaac 4201 ctccgtgaca tcttagagtc ggacttcgcg ttcgcataat actagagcca ggcatcaaat 4261 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 4321 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //