LOCUS       BBa_K233311              945 bp    DNA     linear       11-May-2024
DEFINITION  Protein(GFP) secretion device
ACCESSION   BBa_K233311
VERSION     BBa_K233311.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..219
                     /label=BBa_K233307
     CDS             226..945
                     /label=GFP protein
     CDS             226..945
                     /label=BBa_E0040
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaacaata acgacctgtt ccaggcgtct cgtcgccgtt
      121 ttctggctca actgggtggc ctgaccgttg caggtatgct gggtccgtct ctgctgactc
      181 cgcgccgtgc tactgccgcg caagcggcta ctgacgctct actagatgcg taaaggagaa
      241 gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt taatgggcac
      301 aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact tacccttaaa
      361 tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac tactttcggt
      421 tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga ctttttcaag
      481 agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga tgacgggaac
      541 tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag aatcgagtta
      601 aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga atacaactat
      661 aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa agttaacttc
      721 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
      781 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
      841 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
      901 gctgctggga ttacacatgg catggatgaa ctatacaaat aataa
//