LOCUS       BBa_K233312              929 bp    DNA     linear       11-May-2024
DEFINITION  GFP secretion device using Ycdb
ACCESSION   BBa_K233312
VERSION     BBa_K233312.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..203
                     /label=BBa_K233306
     CDS             210..929
                     /label=GFP protein
     CDS             210..929
                     /label=BBa_E0040
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgcagtaca aagacgaaaa cggtgttaat gagccgtctc
      121 gtcgccgtct gctgaaggtt atcggcgcgc tggctctggc aggttcctgc ccggtggcgc
      181 atgcgcagaa aactcaatct gcctactaga tgcgtaaagg agaagaactt ttcactggag
      241 ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg
      301 gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg
      361 gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct
      421 ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag
      481 gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg
      541 aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta
      601 aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat
      661 acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca
      721 ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg
      781 gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc
      841 ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac
      901 atggcatgga tgaactatac aaataataa
//