BBa_K233004
1
BBa_K233004
lac operator
2009-07-09T11:00:00Z
2015-05-08T01:11:35Z
E.coli
This contains the lacI binding sites without any of the -10 and -35 regions of the promoter.therefore it is only repressed by the binding of LacI
false
false
_329_
0
4258
9
It's complicated
true
had it synthesized
false
Swetha Srinivasan, Samit Watve, Mandar Phatak, Chinar Patil
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K124003
1
BBa_K124003
Bacteriophage 21 Lysis Cassette S, R, and Rz (PVJ4)
2008-07-08T11:00:00Z
2015-05-08T01:09:44Z
This part comes from a Lamda Phage provided to us by Harvard Medical School with the help of Ry Young (Texas A&M).
Induces lysis in E. Coli bacteria.
false
false
_221_
0
3365
9
It's complicated
true
Not many.
true
Neil J. Parikh
BBa_R1062
1
lux pR
Promoter, Standard (luxR and HSL regulated -- lux pR)<br>
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
[Note: This is the same part as R0062 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001].</p> <p>Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
false
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file part=BBa_R0062>Image1.gif</bb_file>" width="614" height="362"><br><br> Modified to comply with BBa_S0001:<br> TTGACA-17N-GATACT<br> <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr<br> Drew Endy<br>
annotation2101
1
-35
range2101
1
20
25
annotation2100
1
start
range2100
1
54
54
annotation7077
1
BBa_R1062
range7077
1
1
56
annotation2099
1
-10
range2099
1
42
48
annotation2098
1
LuxR/HSL
range2098
1
1
20
BBa_K233322
1
BBa_K233322
AHL and Lactose Modulated Cell Lysis
2009-10-17T11:00:00Z
2015-05-08T01:11:35Z
a
a
false
false
_329_
0
4259
9
Not in stock
false
a
false
swetha srinivasan, chinar patil, mandar phatak, samit watve
component2051003
1
BBa_K124003
component2051004
1
BBa_B0010
component2050995
1
BBa_R1062
component2051006
1
BBa_B0012
component2051001
1
BBa_B0030
component2050999
1
BBa_K233004
annotation2050999
1
BBa_K233004
range2050999
1
65
94
annotation2051006
1
BBa_B0012
range2051006
1
1495
1535
annotation2051003
1
BBa_K124003
range2051003
1
126
1398
annotation2050995
1
BBa_R1062
range2050995
1
1
56
annotation2051004
1
BBa_B0010
range2051004
1
1407
1486
annotation2051001
1
BBa_B0030
range2051001
1
103
117
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K124003_sequence
1
aattcatcagtgatagttacgttacgctgcgccttctttttttcccttccaatataagaactacgcaatccgttactggcggaggcgttgctatgaaatcaatggacaaaatctcaactggcattgcctacggaacatctgctggtagtgcgggatactggtttttgcagtggttggatcaggtcagtccgtcacagtgggctgcgattggagtgcttggaagccttgtgttgggttttctcacttatctgacaaatctgtatttcaaaatcagagaagacagacgaaaggctgcgagaggtgaataatgcctccatcattacgaaaagccgttgctgctgctattggtggcggagcaattgctatagcatcagtgttaatcactggcccaagtggtaacgatggtctggaaggtgtcagctacataccatacaaagatattgttggtgtatggactgtatgtcacgggcatacaggaaaagacatcatgctcggtaaaacgtataccaaagcagaatgcaaagccctcctgaataaagaccttgccacggtcgccagacaaattaacccgtacatcaaagtcgatataccggaaacaatgcgcggcgctctttactcattgctttacaacgtgggtgctggcaatttcagaacatcgacgcttcttcgtaaaataaaccagggcgatatcaaaggcgcatgtgatcagctacgtcgctggacatatgctggcggtaagcaatggaaaggtctcatgactcgtcgtgagattgagcgtgaaatctgtttgtggggtcagcaatgaacagagtaaccgcgattatctccgctctggttatctgcatcatcgtctgcctgtcatgggctgttaatcattaccgtgataacgccattacctacaaagcccagcgcgacaaaaatgccagagaactgacgctggcgaaccgggtaattactgacatacagatgcgtcagcgtgatgttgctgcgctcgatgcaaaatacacgaaggagttagctgatgcgaaagctgaaaatgatgctctgcgtgatgatgttgccgctggtcgtcgtcggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccacgactgcctccggcgtggataatgcagcctccccccgactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatgcaaaaacaactggaaggaacccagaagtatattaatgagcagtgcagatagagctgcccat
BBa_K233004_sequence
1
tgtggaattgtgagcgctcacaattccaca
BBa_R1062_sequence
1
acctgtaggatcgtacaggttgacacaagaaaatggtttgttgatactcgaataaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K233322_sequence
1
acctgtaggatcgtacaggttgacacaagaaaatggtttgttgatactcgaataaatactagagtgtggaattgtgagcgctcacaattccacatactagagattaaagaggagaaatactagagaattcatcagtgatagttacgttacgctgcgccttctttttttcccttccaatataagaactacgcaatccgttactggcggaggcgttgctatgaaatcaatggacaaaatctcaactggcattgcctacggaacatctgctggtagtgcgggatactggtttttgcagtggttggatcaggtcagtccgtcacagtgggctgcgattggagtgcttggaagccttgtgttgggttttctcacttatctgacaaatctgtatttcaaaatcagagaagacagacgaaaggctgcgagaggtgaataatgcctccatcattacgaaaagccgttgctgctgctattggtggcggagcaattgctatagcatcagtgttaatcactggcccaagtggtaacgatggtctggaaggtgtcagctacataccatacaaagatattgttggtgtatggactgtatgtcacgggcatacaggaaaagacatcatgctcggtaaaacgtataccaaagcagaatgcaaagccctcctgaataaagaccttgccacggtcgccagacaaattaacccgtacatcaaagtcgatataccggaaacaatgcgcggcgctctttactcattgctttacaacgtgggtgctggcaatttcagaacatcgacgcttcttcgtaaaataaaccagggcgatatcaaaggcgcatgtgatcagctacgtcgctggacatatgctggcggtaagcaatggaaaggtctcatgactcgtcgtgagattgagcgtgaaatctgtttgtggggtcagcaatgaacagagtaaccgcgattatctccgctctggttatctgcatcatcgtctgcctgtcatgggctgttaatcattaccgtgataacgccattacctacaaagcccagcgcgacaaaaatgccagagaactgacgctggcgaaccgggtaattactgacatacagatgcgtcagcgtgatgttgctgcgctcgatgcaaaatacacgaaggagttagctgatgcgaaagctgaaaatgatgctctgcgtgatgatgttgccgctggtcgtcgtcggttgcacatcaaagcagtctgtcagtcagtgcgtgaagccacgactgcctccggcgtggataatgcagcctccccccgactggcagacaccgctgaacgggattatttcaccctcagagagaggctgatcactatgcaaaaacaactggaaggaacccagaagtatattaatgagcagtgcagatagagctgcccattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z