LOCUS       BBa_K235036              983 bp    DNA     linear       22-May-2024
DEFINITION  [K235030][K235005] (constituitive promoter+32??C RBS 
            thermometer+mCherry CDS+stop)
ACCESSION   BBa_K235036
VERSION     BBa_K235036.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23102
     RBS             120..125
                     /label=SD
     misc_feature    44..126
                     /label=BBa_K115017
     CDS             133..843
                     /label=mCherry
     misc_feature    484..484
                     /label=C->T (removing PstI site)
     misc_feature    133..846
                     /label=BBa_J06504
     stem_loop       866..909
                     /label=stem_loop
     misc_feature    855..934
                     /label=BBa_B0010
     terminator      855..934
                     /label=BBa_B0010
     stem_loop       950..969
                     /label=T7 TE
     misc_feature    976..976
                     /label=stop
     polyA_site      970..983
                     /label=polya
     misc_feature    943..983
                     /label=BBa_B0012
     terminator      943..983
                     /label=BBa_B0012
ORIGIN
        1 ttgacagcta gctcagtcct aggtactgtg ctagctacta gagccgggcg cccttcgggg
       61 gcccggcgga gacgggcgcc ggaggtgtcc gacgcctgct cgtccagtct ttgctcagtg
      121 gaggattact agatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      181 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      241 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      301 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      361 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      421 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      481 tccttgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
      541 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
      601 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
      661 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      721 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
      781 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
      841 taataatact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      901 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      961 gggtgggcct ttctgcgttt ata
//