LOCUS       BBa_K237004              307 bp    DNA     linear       09-Jun-2024
DEFINITION  Constitutive sRIP Generator. RIP - Quorum sensing inhibitor of 
            Staphylococcus spp.
ACCESSION   BBa_K237004
VERSION     BBa_K237004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..83
                     /label=Start
     misc_feature    165..169
                     /label=Stop
     CDS             84..143
                     /label=OmpA Leader
     CDS             144..164
                     /label=RIP
     CDS             81..170
                     /label=BBa_K237003
     stem_loop       190..233
                     /label=stem_loop
     misc_feature    179..258
                     /label=BBa_B0010
     terminator      179..258
                     /label=BBa_B0010
     stem_loop       274..293
                     /label=T7 TE
     misc_feature    300..300
                     /label=stop
     polyA_site      294..307
                     /label=polya
     misc_feature    267..307
                     /label=BBa_B0012
     terminator      267..307
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaga cagctatcgc gattgcagtg gcactggctg
      121 gtttcgctac cgtagcgcag gcctattctc cgtggaccaa cttttaataa tactagagcc
      181 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
      241 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
      301 gtttata
//