BBa_R0011 1 lacI+pL Promoter (lacI regulated, lambda pL hybrid) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference). false true _1_ 0 24 7 In stock false <P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs. true Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton annotation7064 1 BBa_R0011 range7064 1 1 54 annotation1999 1 lac O1 range1999 1 3 19 annotation2000 1 -35 range2000 1 20 25 annotation2002 1 -10 range2002 1 43 48 annotation2001 1 lac O1 range2001 1 26 42 BBa_K237006 1 BBa_K237006 IPTG inducible sRIP Generator. RIP - Quorum sensing inhibitor of Staphylococcus spp. 2009-08-02T11:00:00Z 2015-05-08T01:11:36Z The RIP peptide is produced by coagulase negative staphylococcus (suggested to be S. warnerii or S. xylosus). RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species. RIP effectively works as an antibiotic in its inhibition of bacterial growth. RIP has the sequence YSPXTNF and has been shown through extensive research to inhibit bacterial infections in mice in a synthetic (as in organic synthesis) form. The RIP produced by this generator is an export-tagged RIP brick. We used an OmpA tag, which (at least in theory) targets the peptide to the periplasmic space from where, there has been described numerous cases of leakage into the medium. Under the transport into the periplasmic space, the OmpA-leader should be cut off, leaving the RIP intact, and ready to inhibit some staph's. References will come later. false false _333_ 0 4087 9 Not in stock false There are 4 versions of RIP producers from us. 1 - Constitutively produced RIP without an export sequence 2 - Inducibly produced RIP without an export sequence 3 - Constitutively produced RIP with an export sequence (OmpA) 4 - Inducibly produced RIP with an export sequence (OmpA) This is number 4 false Marc T. K. Nielsen component2014697 1 BBa_B0034 component2014703 1 BBa_B0010 component2014691 1 BBa_R0011 component2014702 1 BBa_K237003 component2014705 1 BBa_B0012 annotation2014702 1 BBa_K237003 range2014702 1 82 171 annotation2014703 1 BBa_B0010 range2014703 1 180 259 annotation2014705 1 BBa_B0012 range2014705 1 268 308 annotation2014691 1 BBa_R0011 range2014691 1 1 54 annotation2014697 1 BBa_B0034 range2014697 1 64 75 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K237003 1 sRIP RIP +export signal (OmpA). Quorum sensing inhibitor of Staphylococcus spp. (Including MRSA) 2009-08-02T11:00:00Z 2015-05-08T01:11:36Z The RIP peptide is produced by coagulase negative staphylococcus (suggested to be S. warnerii or S. xylosus) (16,19) RIP or RNAIII inhibiting peptide is a heptapeptide capable of severely reducing the Qourum sensing(QS) abilities of Staphylococcus species. RIP effectively works as an antibiotic in its inhibition of bacterial growth. RIP has the sequence YSPXTNF and has been tested extensively in a synthetic (as in organic) form for ability to inhibit bacterial infections in mice. References will come later. This is an export-tagged RIP brick. We used an OmpA tag, which (at least in theory) targets the peptide to the periplasmic space from where, there has been described numerous cases of leakage into the medium. Under the transport into the periplasmic space, the OmpA-leader should be cut off, leaving the RIP intact, and ready to inhibit some staph's. We can at this point in our project not verify that RIP will be exported by our Coli's. We have come to affectionately call this brick sRIP (short for signal-RIP, in contrast to just RIP). false false _333_ 0 4087 9 It's complicated true This sequence is reverse-engineered from the amino-acid sequence by using the commonly used codons of E. Coli. It is designed to provide a big output of peptide. We just hope that this output won't kill the cell. false Marc T. K. Nielsen annotation2014671 1 OmpA Leader range2014671 1 4 63 annotation2014670 1 Stop range2014670 1 85 89 annotation2014669 1 Start range2014669 1 1 3 annotation2014672 1 RIP range2014672 1 64 84 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K237006_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagaaagaggagaaatactagatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggcctattctccgtggaccaacttttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0034_sequence 1 aaagaggagaaa BBa_K237003_sequence 1 atgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggcctattctccgtggaccaacttttaataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_R0011_sequence 1 aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z