BBa_K237008

BBa_K237008 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K237008

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Marc T. K. Nielsen

Date created: 2009-10-20 11:00:00

Date modified: 2015-05-08 01:11:36

OmpA leader sequence - Targeting to periplasm, cleaved during transport

Details

• Types: DnaRegion
• Roles: Tag; tag
• Sequences: BBa_K237008_sequence (Version 1)

Description

This part is a leader sequence containing a localisation signal to the periplasm of a bacteria. The leader sequence should be cleaved off from the primary transcript during transport, leaving your tagged peptide/protein intact.

Amino acid sequence: MKKTAIAIAVALAGFATVAQA

Reference:

Recombinant protein secretion in Escherichia coli.
F J M Mergulh??o, D K Summers, and G A Monteiro
Biotechnology advances 23 (3), 177-202 (May 2005)
info:pmid/15763404 | info:doi/10.1016/j.biotechadv.2004.11.003

Notes

We chose this localisation signal for use with our

Source

http://www.pharmcast.com/Patents/Yr2003/May2003/052003/6566108_Cytochrome052003.htm

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Start	1,3	feature/start, start_codon
OmpA leader	4,65	tag, feature/tag

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K237008/1

Member of these Collections

• iGEM Parts Registry