BBa_J15001
1
BBa_J15001
strong synthetic E. coli ribosome binding site with SacI site.
2007-07-12T11:00:00Z
2015-08-31T04:08:32Z
Synthetic.
This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
false
false
_163_
0
837
163
Not in stock
false
Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
false
Chris French
annotation1938045
1
SacI
range1938045
1
1
3
annotation1938046
1
rbs
range1938046
1
4
10
BBa_J23078
1
BBa_J23078
[lock3i]
2006-09-12T11:00:00Z
2015-08-31T04:08:39Z
<tt>
PCR ca1028F/G00101 on pJ23006-J23032 . . . (162 bp, XbaI/PstI)<br>
Sub into pSB1A2-I13521 . . . . . . . . . . (XbaI/PstI)<br>
Product is pSB1A2-J23078<br>
</tt>
lock3d derivative with a 5' extension. This is the basic part, J23077 is the RFP reporter.
false
true
_52_
0
483
95
It's complicated
false
N/A
false
John Anderson
BBa_J23110
1
BBa_J23110
constitutive promoter family member
2006-08-16T11:00:00Z
2015-08-31T04:08:40Z
Later
Later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_I742114
1
BBa_I742114
Feruloyl-CoA synthase (fcs)
2007-10-18T11:00:00Z
2015-08-31T04:08:02Z
Pseudomonas fluorescens NCIMB 9046
Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)
gene is from Pseudomonas fluorescens
encodes feruoyl CoA synthase (ligates acetyl-CoA onto ferulic acid)
produces feruloyl CoA from ferrulic acid
catalyses the penultimate of the vanillin biosynthesis pathway
false
false
_123_
0
1964
9
Not in stock
false
Gene was synthesised by geneart, as P. fluorescens has a high GC genome.
Coding region was optimised for gene expression in E. coli
false
sarah hollingshead
annotation1949792
1
fcs coding region
range1949792
1
1
1773
BBa_K238007
1
rbs + sam5
Sam5 gene with RBS
2009-07-26T11:00:00Z
2015-05-08T01:11:36Z
lab of Prof. C. French of the University of Edinburgh
This is an improved version of the Sam5 gene (BBa_I742105). The restriction site for PstI that was initially found at location 1436 was mutated out. The mutagenesis was done by Mr. Rashed Al Naber under supervision of Prof. C. French of the University of Edinburgh, in March 2008, using BioBrick BBa_I742105 as a template (this BioBrick was prepared by the Edinburgh iGEM 2007 team but was not submitted to the Registry).
false
false
_332_
0
4771
9
It's complicated
true
the PstI restriction site was mutated out.
false
Annelien Verfaillie
annotation2013241
1
sam5
range2013241
1
17
1558
annotation2013240
1
rbs
range2013240
1
1
16
BBa_I742107
1
COMT
COMT
2007-10-18T11:00:00Z
2015-08-31T04:08:02Z
Alfalfa cDNA
Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix esanaensis, Berner M, et al, Journal of Bacteriology 188(7): 2666, (2006)
Gowri G, et al, Molecular cloning and expression of alfalfa S-adenosyl-L-methionine: caffeic acid 3-0-methyltransferase, a key enzyme of lignin biosynthesis, Plant Physiology 97(1):7 (1991)
Downregulation of Caffeic Acid 3-O-Methyltransferase and Caffeoyl CoA 3-O-Methyltransferase in Transgenic Alfalfa Impacts on Lignin Structure and Implications for the Biosynthesis of G and S Lignin, Guo D, et al, Plant Cell. January; 13(1): 73 (2001)
Released HQ 2013
COMT coding sequence
Encodes caffeic acid-O-methyl transferase (methylates -OH on C4 of the aromatic ring) from Medicago sativa (alfalfa), synthesised by Geneart
false
false
_123_
0
1964
9
In stock
true
Used cDNA as coding gene in Alfalfa genome contains introns.
true
sarah hollingshead
annotation1949817
1
COMT coding region
range1949817
1
1
1101
BBa_I742112
1
BBa_I742112
enoyl CoA hydratase (ech) gene
2007-10-18T11:00:00Z
2015-08-31T04:08:02Z
Pseudomonas fluorescens NCIMB 9046
Cloning and Characterization of the Ferulic Acid Catabolic Genes of Sphingomonas paucimobilis SYK-6, E Masai, et al, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(9): 4416???4424 (2002)
enoyl CoA hydratase (ech) was isolated from Pseudomonas fluorescens genome.
Ech converts feruloyl CoA to vanillin (fig. 3) and catalyses the final stage of the vanillin biosynthesis pathway.
Involved in the synthesis of 'natural' vanilla flavouring
false
false
_123_
0
1964
9
Not in stock
false
Gene was synthesised by geneart as we experienced problems isolating native gene, due to the high GC nature of the Pseudomonas genome.
The ech sequence has been optimised for expression in E. coli
false
sarah hollingshead
annotation1949783
1
start
range1949783
1
1
3
annotation1949784
1
stop
range1949784
1
829
834
annotation1949782
1
ech coding region
range1949782
1
1
828
BBa_K238004
1
BBa_K238004
Vanillin synthesis
2009-07-14T11:00:00Z
2015-05-08T01:11:36Z
most parts were found in the registry.
this part is composed of several genes coding for 5 different enzymes which turn tyrosine into vanilla
false
false
_332_
0
4771
9
Not in stock
true
parts for fcs (BBa_I742114) and ech (BBa_I742112) will probably not be used and made from another sequence.
false
Annelien Verfaillie
component2013312
1
BBa_J15001
component2013297
1
BBa_J23078
component2013304
1
BBa_I742107
component2013296
1
BBa_J23110
component2013316
1
BBa_I742112
component2013309
1
BBa_I742114
component2013317
1
BBa_B0010
component2013298
1
BBa_I742142
component2013301
1
BBa_K238007
component2013319
1
BBa_B0012
component2013302
1
BBa_J23078
component2013307
1
BBa_J15001
annotation2013307
1
BBa_J15001
range2013307
1
4379
4388
annotation2013317
1
BBa_B0010
range2013317
1
7034
7113
annotation2013319
1
BBa_B0012
range2013319
1
7122
7162
annotation2013316
1
BBa_I742112
range2013316
1
6192
7025
annotation2013297
1
BBa_J23078
range2013297
1
44
95
annotation2013309
1
BBa_I742114
range2013309
1
4395
6167
annotation2013302
1
BBa_J23078
range2013302
1
3212
3263
annotation2013296
1
BBa_J23110
range2013296
1
1
35
annotation2013304
1
BBa_I742107
range2013304
1
3270
4370
annotation2013298
1
BBa_I742142
range2013298
1
102
1637
annotation2013312
1
BBa_J15001
range2013312
1
6176
6185
annotation2013301
1
BBa_K238007
range2013301
1
1646
3203
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_I742142
1
sam8
sam8 (tyrosine-ammonia lyase) coding sequence
2007-10-22T11:00:00Z
2015-08-31T04:08:03Z
Saccharothrix espanaensis DSM 4429. Accession: ABC88666.
This is the coding sequence of sam8, encoding tyrosine ammonia lyase, from the bacterium Saccharothrix espanaensis DSM 4429. The enzyme converts tyrosine to p-coumaric acid. Accession: ABC88666. Reference: Berner, M., Krug, D., Bihlmaier, C., Vente, A., Muller, R. and Bechthold, A. 'Genes and Enzymes Involved in Caffeic Acid Biosynthesis in the Actinomycete Saccharothrix espanaensis' J. Bacteriol. 188 (7), 2666-2673 (2006)
false
false
_123_
0
837
163
It's complicated
true
No important considerations.
false
sarah hollingshead
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K238007_sequence
1
acggagaagcagcgaaatgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccggactacaaggcggcgttcttcggcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctggtaccgcgacgcccaggaacgggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgccgaccggaccgccgacatctgcgtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcgcgaccggctccgcgatgaccaacgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggtgttcacggtcccgatgaactcgcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggctcgcccttcgactacccgctgtcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccctgggagaacgtgttcatgtacgacgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcaccttccacggctgcacccgcctcgcggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcaccacgcacttccggggcgtgcaggcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcgatggccaagtcgccgaactcgtgggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcatgggcgtgggctacccgcgcatcaaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggccgccgactggaagaaccccgacgtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgaccgggtcaagctgctgaagctgctgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaactacggcggcgaccacgagggcatccgggtgcagaccctgcaagcgtaccaggcgaacggccaagccgccgcgctcaagggtttcgccgagcagtgcatgtccgagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataa
BBa_J23078_sequence
1
tgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggaga
BBa_I742107_sequence
1
atgggttcaacaggtgaaactcaaataacaccaacccacatatcagatgaagaagcaaacctcttcgccatgcaactagcaagtgcttcagttcttcccatgattttgaaatcagctcttgaacttgatctcttagaaatcattgctaaagctggacctggtgctcaaatttcacctattgaaattgcttctcagctaccaacaactaaccctgatgcaccagttatgttggaccgaatgttgcgtctcttggcttgttacataatcctcacatgttcagttcgtactcaacaagatggaaaggttcagagactttatggtttggctactgttgctaagtatttggttaagaatgaagatggtgtatccatttctgctcttaatctcatgaatcaggataaagtgctcatggaaagctggtaccacctaaaagatgcagtccttgatgggggcattccattcaacaaggcttatggaatgacagcctttgaataccatggaacagatccaaggtttaacaaggttttcaacaaggggatgtctgatcactctaccatcacaatgaagaaaattcttgagacctacacaggttttgaaggccttaaatctcttgttgatgtaggtggtggtactggagctgtaattaacacgattgtctcaaaatatcccactataaagggtataaattttgatttaccccatgtcattgaagatgctccatcttatccaggagttgagcatgttggtggagacatgtttgtcagtattccaaaggctgatgctgtttttatgaagtggatttgtcatgactggagtgatgagcactgcttgaaatttttgaagaactgctatgaggcactgccagacaatggaaaagtgattgtggcagaatgcatacttccagtggctccagattcaagcctggccacaaaaggtgtggttcacattgatgtgatcatgttggctcataatcctggtgggaaagagagaacacaaaaagagtttgaggatcttgccaaaggtgctggattccaaggtttcaaagtccattgtaatgctttcaacacatacatcatggagtttcttaagaaggtttaataa
BBa_I742112_sequence
1
atgagcaaatatgaaggccgttggaccaccgtgaaagtggaaattgaagaaggcattgcctgggttattctgaaccgtccggaaaaacgtaacgccatgagcccgaccctgaaccgtgaaatgattgatgtgctggaaaccctggaacaggatccggcggcgggtgttctggttctgaccggtgcgggtgaagcgtggaccgcgggcatggatctgaaagaatatttccgtgaagtggatgcgggtccggaaattctgcaagaaaaaattcgtcgcgaagcgagccagtggcagtggaaactgctgcgtatgtatgcgaaaccgaccattgccatggtgaacggctggtgctttggcggcggttttagcccgctggttgcgtgcgatctggccatttgcgcggatgaagcgacctttggcctgagcgagattaactggggcattccgccgggtaacctggtgagcaaagccatggcggataccgtgggccatcgtcagagcctgtattatatcatgaccggcaaaacctttggtggccagaaagcggcggaaatgggcctggtgaacgaaagcgtgccgctggcccagctgcgtgaagtgaccattgaactggcccgtaacctgctggaaaaaaatccggtggtgctgcgtgcggcgaaacatggctttaaacgttgccgtgaactgacctgggaacagaacgaagattatctgtacgcgaaactggatcagagccgtctgctggataccgaaggcggccgtgaacagggcatgaaacagtttctggatgataaaagcattaaaccgggcctgcaagcgtataaacgttaataa
BBa_J15001_sequence
1
ctcaaggagg
BBa_K238004_sequence
1
tttacggctagctcagtcctaggtacaatgctagctactagagtgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgacgcaggtcgtggaacgtcaggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggtgggacgccggtctcacctcgtgcaccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacctgaagtccgacaagcccatctacggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggagcagggcggctcgctgatctcgcacctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctctggctgcgcatccagaacatgcgcaaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaacaaggggttcaccccggcgatcccccggcacggcacggtcagcgcgagcggcgacctgcaaccgctggcgcacgccgcgctcgccttcaccggtgtcggcgaggcgtggacccgggacgccgacggccggtggtccaccgtgccggccgtggacgcgctcgccgcgctgggggcggagccgttcgactggccggtgcgcgaggcgctggcgttcgtcaacgggaccggcgcgagcctcgcggtggctgtgctcaaccaccggtccgccctgcggctggtccgcgcctgcgccgtgctctccgcgcggctggcgaccctgctgggggccaatcccgagcactacgacgtggggcacggtgtcgcgcgcggccaggtcggtcagctgaccgcggcggagtggatccggcaggggctgccccggggcatggtgcgcgacggcagccgcccgctccaggagccgtacagcctgcggtgcgcgccgcaggtgctcggcgcggtgctcgaccagctcgacggcgcgggcgacgtgctggcgcgggaggtcgacggctgccaggacaacccgatcacctacgagggcgagctgctgcacggcggcaacttccacgccatgccggtgggtttcgcctccgaccagatcgggttggccatgcacatggccgcctacctggccgagcgccagctgggtctgctggtcagcccggtgaccaacggcgacctgccgcccatgctcaccccgcgcgccgggcgcggtgccgggctggccggggtgcagatcagcgcgacctcgttcgtctcgcggatccggcagctggtgttccccgcctcgctgaccaccctgccgaccaacggctggaaccaggaccacgtgccgatggcgctcaacggggcgaactcggtgttcgaggcgttggagctcggctggctgacggtcgggtcgctggcggtgggcgtcgcgcagctcgcggccatgaccggccacgccgcggagggcgtctgggcggagctggccgggatctgcccgccgctggacgccgaccgcccgctgggcgccgaggtgcgcgccgcgcgcgacctgctgtccgcgcacgcggaccaactgctcgtcgacgaggcagacgggaaggatttcggataataatactagagacggagaagcagcgaaatgaccatcacgtcacctgcgccggcgggccggctcaacaacgtccgcccgatgacgggcgaggagtacctggaatcactgcgggacggccgagaggtctacatctacggcgagcgggtcgacgacgtcaccacgcacctggccttccgcaacagcgtgcgctccatcgcgcggctctacgacgtgctgcacgacccggcgtccgaaggtgtgctgcgggtgcccaccgacaccggcaacggcgggttcacccacccgttcttcaagaccgcccggtcgtcggaggacctggtcgccgcgcgcgaggccatcgtcggctggcagcggctggtgtacgggtggatgggccgcaccccggactacaaggcggcgttcttcggcacgctcgacgccaacgccgagttctacgggccgttcgaggccaacgcccgccgctggtaccgcgacgcccaggaacgggtgctgtacttcaaccacgcgatcgtgcacccgccggtcgaccgggaccggcccgccgaccggaccgccgacatctgcgtgcacgtggaggaggagaccgacagcgggttgatcgtctccggcgccaaggtggtcgcgaccggctccgcgatgaccaacgcgaacctcatcgcgcactacgggcttccggtgcgggacaagaagttcggcctggtgttcacggtcccgatgaactcgcccggcctcaagctcatctgccgcacctcctacgagctgatggtcgcgacgcagggctcgcccttcgactacccgctgtcgagccggctcgacgagaacgactcgatcatgatcttcgaccgggtgctggtgccctgggagaacgtgttcatgtacgacgcgggcgcggccaactccttcgccaccgggtcaggcttcctcgaacgcttcaccttccacggctgcacccgcctcgcggtcaagctggacttcatcgccggctgcgtcatgaaggcggtggaggtcaccggcaccacgcacttccggggcgtgcaggcgcaggtcggcgaagtgctcaactggcgcgacgtgttctggggcctgtccgacgcgatggccaagtcgccgaactcgtgggtcggcggctcggtgcagccgaacctcaactacgggctcgcgtaccgcaccttcatgggcgtgggctacccgcgcatcaaggagatcatccagcagaccctcggcagcgggttgatctacctgaactcctcggccgccgactggaagaaccccgacgtccgcccgtacctcgaccgctacctgcgcggctcgcggggcatccaggcgatcgaccgggtcaagctgctgaagctgctgtgggacgcggtcggcaccgagttcgccggccggcacgagctctacgagcgcaactacggcggcgaccacgagggcatccgggtgcagaccctgcaagcgtaccaggcgaacggccaagccgccgcgctcaagggtttcgccgagcagtgcatgtccgagtacgacctcgacggctggacgaggcccgacctgatcaaccccggcacctaataatactagagtgtagaccgaactagaatcacctcttgcttttgggtaagacagaagaggagatactagatgggttcaacaggtgaaactcaaataacaccaacccacatatcagatgaagaagcaaacctcttcgccatgcaactagcaagtgcttcagttcttcccatgattttgaaatcagctcttgaacttgatctcttagaaatcattgctaaagctggacctggtgctcaaatttcacctattgaaattgcttctcagctaccaacaactaaccctgatgcaccagttatgttggaccgaatgttgcgtctcttggcttgttacataatcctcacatgttcagttcgtactcaacaagatggaaaggttcagagactttatggtttggctactgttgctaagtatttggttaagaatgaagatggtgtatccatttctgctcttaatctcatgaatcaggataaagtgctcatggaaagctggtaccacctaaaagatgcagtccttgatgggggcattccattcaacaaggcttatggaatgacagcctttgaataccatggaacagatccaaggtttaacaaggttttcaacaaggggatgtctgatcactctaccatcacaatgaagaaaattcttgagacctacacaggttttgaaggccttaaatctcttgttgatgtaggtggtggtactggagctgtaattaacacgattgtctcaaaatatcccactataaagggtataaattttgatttaccccatgtcattgaagatgctccatcttatccaggagttgagcatgttggtggagacatgtttgtcagtattccaaaggctgatgctgtttttatgaagtggatttgtcatgactggagtgatgagcactgcttgaaatttttgaagaactgctatgaggcactgccagacaatggaaaagtgattgtggcagaatgcatacttccagtggctccagattcaagcctggccacaaaaggtgtggttcacattgatgtgatcatgttggctcataatcctggtgggaaagagagaacacaaaaagagtttgaggatcttgccaaaggtgctggattccaaggtttcaaagtccattgtaatgctttcaacacatacatcatggagtttcttaagaaggtttaataatactagagctcaaggaggtactagatgcgtagcctggaaccgctggccgaactgccggcacgtctgctggatcgtctggtgcattgggcgcgtgtgcgtccggaacagacctttattgcggcgcgtcaggcgggtggtgattggcgtcgtgtgagctatcgcgaaatgctggatggcgtgcgtgcgattgcgcagggcctgctgtcttatggcctgagcgcggataaaccgctggccctgctgtctggcaacgatattgaacatctgcaagtggcgctgggcgcaatgtatgcgggcattccgtattgcccggtgagcccggcgtatagcctgctgtctcaggattttgcgaaactgcgtcatgtgtgcgatctgctgcaaccgggcctggtttttgtgtctgatgcgccggcatatcagcgtgcgattgatgcggttctgccgccggaaaccccgctgattagcgtgcgtggccaggttccgggccgtacgcagattagctttgcgagcctgctgcataccagcgcgggcaccgaagcggatgcggcgtttaacgcgaccggtccggatagcattgcgaaatttctgtttaccagcggcagcaccaaactgccgaaagcggtgattaccacccagcgtatgctgtgcgcgaaccagcagatgctgctgcaaacctttccggtgtttggcgaagaaccgccggtgctggttgattggctgccgtggaaccacacctttggcggcagccataacgtgggcattgtgctgtataacggcggcaccttttatctggatgatggcaaaccgaccgcccagggctttgcacagacgctgcgcaacctgaaagaaattagcccgaccgcgtatctgaccgtgccgaaaggctgggaagaactggtgaacgcgctggaacaggatgcggcgctgcgtgaacgtttttttgcgcgcatgaaactgtttttttttgcggcggcaggcctgagccagagcgtgtgggatcgtctggatcgtgtggcggaacaacattgtggcgaacgtattcgcatgatggcgggcctgggcatgaccgaagcggcaccgagctgcacctttaccaccggtccgctgtctatggcgggctatattggcctgccggcaccgggttgtgaagtgcgtctggtgccggtggatggcaaatttgaaggccgttttcgtggcccgcatatcatgccgggttattggcgtgcaccggaacagaccgcggaagtgtttgatgaacagggctattattgcagcggtgatgcgatcaaactggccgatccgcatcagccgcattggggcctgatgtttgatggccgtattgcggaagattttaaactgagcagcggcgtgtttgtgtccgtgggcccgctgcgtaatcgtgcggttctggaaggcagcccgtatgtgcaggatctggtgattgcggcaccggatcgtgaatgcctgggcgccctggtttttccgcgtgcgtatgaatgccgtcgtctgagcggcctgaacgcggaagcgtctgatgcggaagtgctgaacagcgcaccggttcgtcagtggtttggcgattggctgcaacgtctgaaccgtgaagcgaccggcaatgcgagccgtctggaatggatcgcggtgctggatgaaccggcgagcattgatcgtggcgaaattaccgataaaggcagcattaaccagcgcgcggtgctgcaatggcgtgcggcgaaagtggaagcgctgtatcgtggcgaagatccgaccattctgcgtgcgggtccgaaaccgtaataatactagagctcaaggaggtactagatgagcaaatatgaaggccgttggaccaccgtgaaagtggaaattgaagaaggcattgcctgggttattctgaaccgtccggaaaaacgtaacgccatgagcccgaccctgaaccgtgaaatgattgatgtgctggaaaccctggaacaggatccggcggcgggtgttctggttctgaccggtgcgggtgaagcgtggaccgcgggcatggatctgaaagaatatttccgtgaagtggatgcgggtccggaaattctgcaagaaaaaattcgtcgcgaagcgagccagtggcagtggaaactgctgcgtatgtatgcgaaaccgaccattgccatggtgaacggctggtgctttggcggcggttttagcccgctggttgcgtgcgatctggccatttgcgcggatgaagcgacctttggcctgagcgagattaactggggcattccgccgggtaacctggtgagcaaagccatggcggataccgtgggccatcgtcagagcctgtattatatcatgaccggcaaaacctttggtggccagaaagcggcggaaatgggcctggtgaacgaaagcgtgccgctggcccagctgcgtgaagtgaccattgaactggcccgtaacctgctggaaaaaaatccggtggtgctgcgtgcggcgaaacatggctttaaacgttgccgtgaactgacctgggaacagaacgaagattatctgtacgcgaaactggatcagagccgtctgctggataccgaaggcggccgtgaacagggcatgaaacagtttctggatgataaaagcattaaaccgggcctgcaagcgtataaacgttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I742114_sequence
1
atgcgtagcctggaaccgctggccgaactgccggcacgtctgctggatcgtctggtgcattgggcgcgtgtgcgtccggaacagacctttattgcggcgcgtcaggcgggtggtgattggcgtcgtgtgagctatcgcgaaatgctggatggcgtgcgtgcgattgcgcagggcctgctgtcttatggcctgagcgcggataaaccgctggccctgctgtctggcaacgatattgaacatctgcaagtggcgctgggcgcaatgtatgcgggcattccgtattgcccggtgagcccggcgtatagcctgctgtctcaggattttgcgaaactgcgtcatgtgtgcgatctgctgcaaccgggcctggtttttgtgtctgatgcgccggcatatcagcgtgcgattgatgcggttctgccgccggaaaccccgctgattagcgtgcgtggccaggttccgggccgtacgcagattagctttgcgagcctgctgcataccagcgcgggcaccgaagcggatgcggcgtttaacgcgaccggtccggatagcattgcgaaatttctgtttaccagcggcagcaccaaactgccgaaagcggtgattaccacccagcgtatgctgtgcgcgaaccagcagatgctgctgcaaacctttccggtgtttggcgaagaaccgccggtgctggttgattggctgccgtggaaccacacctttggcggcagccataacgtgggcattgtgctgtataacggcggcaccttttatctggatgatggcaaaccgaccgcccagggctttgcacagacgctgcgcaacctgaaagaaattagcccgaccgcgtatctgaccgtgccgaaaggctgggaagaactggtgaacgcgctggaacaggatgcggcgctgcgtgaacgtttttttgcgcgcatgaaactgtttttttttgcggcggcaggcctgagccagagcgtgtgggatcgtctggatcgtgtggcggaacaacattgtggcgaacgtattcgcatgatggcgggcctgggcatgaccgaagcggcaccgagctgcacctttaccaccggtccgctgtctatggcgggctatattggcctgccggcaccgggttgtgaagtgcgtctggtgccggtggatggcaaatttgaaggccgttttcgtggcccgcatatcatgccgggttattggcgtgcaccggaacagaccgcggaagtgtttgatgaacagggctattattgcagcggtgatgcgatcaaactggccgatccgcatcagccgcattggggcctgatgtttgatggccgtattgcggaagattttaaactgagcagcggcgtgtttgtgtccgtgggcccgctgcgtaatcgtgcggttctggaaggcagcccgtatgtgcaggatctggtgattgcggcaccggatcgtgaatgcctgggcgccctggtttttccgcgtgcgtatgaatgccgtcgtctgagcggcctgaacgcggaagcgtctgatgcggaagtgctgaacagcgcaccggttcgtcagtggtttggcgattggctgcaacgtctgaaccgtgaagcgaccggcaatgcgagccgtctggaatggatcgcggtgctggatgaaccggcgagcattgatcgtggcgaaattaccgataaaggcagcattaaccagcgcgcggtgctgcaatggcgtgcggcgaaagtggaagcgctgtatcgtggcgaagatccgaccattctgcgtgcgggtccgaaaccgtaataa
BBa_J23110_sequence
1
tttacggctagctcagtcctaggtacaatgctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_I742142_sequence
1
atgacgcaggtcgtggaacgtcaggctgatcggctcagcagcagggagtacctggcccgggtcgtgcgcagcgccgggtgggacgccggtctcacctcgtgcaccgacgaggagatcgtccggatgggcgcgagcgcgcgcaccatcgaggagtacctgaagtccgacaagcccatctacggcctgacgcagggcttcggtccgctggtgctgttcgacgccgactcggagctggagcagggcggctcgctgatctcgcacctgggcaccggccagggcgcgccactggccccggaggtgtcgcggctgatcctctggctgcgcatccagaacatgcgcaaggggtactcggcggtctcgccggtgttctggcagaagctcgccgacctgtggaacaaggggttcaccccggcgatcccccggcacggcacggtcagcgcgagcggcgacctgcaaccgctggcgcacgccgcgctcgccttcaccggtgtcggcgaggcgtggacccgggacgccgacggccggtggtccaccgtgccggccgtggacgcgctcgccgcgctgggggcggagccgttcgactggccggtgcgcgaggcgctggcgttcgtcaacgggaccggcgcgagcctcgcggtggctgtgctcaaccaccggtccgccctgcggctggtccgcgcctgcgccgtgctctccgcgcggctggcgaccctgctgggggccaatcccgagcactacgacgtggggcacggtgtcgcgcgcggccaggtcggtcagctgaccgcggcggagtggatccggcaggggctgccccggggcatggtgcgcgacggcagccgcccgctccaggagccgtacagcctgcggtgcgcgccgcaggtgctcggcgcggtgctcgaccagctcgacggcgcgggcgacgtgctggcgcgggaggtcgacggctgccaggacaacccgatcacctacgagggcgagctgctgcacggcggcaacttccacgccatgccggtgggtttcgcctccgaccagatcgggttggccatgcacatggccgcctacctggccgagcgccagctgggtctgctggtcagcccggtgaccaacggcgacctgccgcccatgctcaccccgcgcgccgggcgcggtgccgggctggccggggtgcagatcagcgcgacctcgttcgtctcgcggatccggcagctggtgttccccgcctcgctgaccaccctgccgaccaacggctggaaccaggaccacgtgccgatggcgctcaacggggcgaactcggtgttcgaggcgttggagctcggctggctgacggtcgggtcgctggcggtgggcgtcgcgcagctcgcggccatgaccggccacgccgcggagggcgtctgggcggagctggccgggatctgcccgccgctggacgccgaccgcccgctgggcgccgaggtgcgcgccgcgcgcgacctgctgtccgcgcacgcggaccaactgctcgtcgacgaggcagacgggaaggatttcggataataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z