LOCUS       BBa_K238018             2827 bp    DNA     linear       19-May-2024
DEFINITION  EF ligation with promotor and terminator
ACCESSION   BBa_K238018
VERSION     BBa_K238018.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23110
     misc_feature    44..46
                     /label=SacI
     RBS             47..53
                     /label=rbs
     RBS             44..53
                     /label=BBa_J15001
     CDS             60..1832
                     /label=fcs coding region
     CDS             60..1832
                     /label=BBa_I742114
     misc_feature    1841..1843
                     /label=SacI
     RBS             1844..1850
                     /label=rbs
     RBS             1841..1850
                     /label=BBa_J15001
     CDS             1857..2684
                     /label=ech coding region
     misc_feature    1857..1859
                     /label=start
     misc_feature    2685..2690
                     /label=stop
     CDS             1857..2690
                     /label=BBa_I742112
     stem_loop       2710..2753
                     /label=stem_loop
     misc_feature    2699..2778
                     /label=BBa_B0010
     terminator      2699..2778
                     /label=BBa_B0010
     stem_loop       2794..2813
                     /label=T7 TE
     misc_feature    2820..2820
                     /label=stop
     polyA_site      2814..2827
                     /label=polya
     misc_feature    2787..2827
                     /label=BBa_B0012
     terminator      2787..2827
                     /label=BBa_B0012
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagctcaagg aggtactaga
       61 tgcgtagcct ggaaccgctg gccgaactgc cggcacgtct gctggatcgt ctggtgcatt
      121 gggcgcgtgt gcgtccggaa cagaccttta ttgcggcgcg tcaggcgggt ggtgattggc
      181 gtcgtgtgag ctatcgcgaa atgctggatg gcgtgcgtgc gattgcgcag ggcctgctgt
      241 cttatggcct gagcgcggat aaaccgctgg ccctgctgtc tggcaacgat attgaacatc
      301 tgcaagtggc gctgggcgca atgtatgcgg gcattccgta ttgcccggtg agcccggcgt
      361 atagcctgct gtctcaggat tttgcgaaac tgcgtcatgt gtgcgatctg ctgcaaccgg
      421 gcctggtttt tgtgtctgat gcgccggcat atcagcgtgc gattgatgcg gttctgccgc
      481 cggaaacccc gctgattagc gtgcgtggcc aggttccggg ccgtacgcag attagctttg
      541 cgagcctgct gcataccagc gcgggcaccg aagcggatgc ggcgtttaac gcgaccggtc
      601 cggatagcat tgcgaaattt ctgtttacca gcggcagcac caaactgccg aaagcggtga
      661 ttaccaccca gcgtatgctg tgcgcgaacc agcagatgct gctgcaaacc tttccggtgt
      721 ttggcgaaga accgccggtg ctggttgatt ggctgccgtg gaaccacacc tttggcggca
      781 gccataacgt gggcattgtg ctgtataacg gcggcacctt ttatctggat gatggcaaac
      841 cgaccgccca gggctttgca cagacgctgc gcaacctgaa agaaattagc ccgaccgcgt
      901 atctgaccgt gccgaaaggc tgggaagaac tggtgaacgc gctggaacag gatgcggcgc
      961 tgcgtgaacg tttttttgcg cgcatgaaac tgtttttttt tgcggcggca ggcctgagcc
     1021 agagcgtgtg ggatcgtctg gatcgtgtgg cggaacaaca ttgtggcgaa cgtattcgca
     1081 tgatggcggg cctgggcatg accgaagcgg caccgagctg cacctttacc accggtccgc
     1141 tgtctatggc gggctatatt ggcctgccgg caccgggttg tgaagtgcgt ctggtgccgg
     1201 tggatggcaa atttgaaggc cgttttcgtg gcccgcatat catgccgggt tattggcgtg
     1261 caccggaaca gaccgcggaa gtgtttgatg aacagggcta ttattgcagc ggtgatgcga
     1321 tcaaactggc cgatccgcat cagccgcatt ggggcctgat gtttgatggc cgtattgcgg
     1381 aagattttaa actgagcagc ggcgtgtttg tgtccgtggg cccgctgcgt aatcgtgcgg
     1441 ttctggaagg cagcccgtat gtgcaggatc tggtgattgc ggcaccggat cgtgaatgcc
     1501 tgggcgccct ggtttttccg cgtgcgtatg aatgccgtcg tctgagcggc ctgaacgcgg
     1561 aagcgtctga tgcggaagtg ctgaacagcg caccggttcg tcagtggttt ggcgattggc
     1621 tgcaacgtct gaaccgtgaa gcgaccggca atgcgagccg tctggaatgg atcgcggtgc
     1681 tggatgaacc ggcgagcatt gatcgtggcg aaattaccga taaaggcagc attaaccagc
     1741 gcgcggtgct gcaatggcgt gcggcgaaag tggaagcgct gtatcgtggc gaagatccga
     1801 ccattctgcg tgcgggtccg aaaccgtaat aatactagag ctcaaggagg tactagatga
     1861 gcaaatatga aggccgttgg accaccgtga aagtggaaat tgaagaaggc attgcctggg
     1921 ttattctgaa ccgtccggaa aaacgtaacg ccatgagccc gaccctgaac cgtgaaatga
     1981 ttgatgtgct ggaaaccctg gaacaggatc cggcggcggg tgttctggtt ctgaccggtg
     2041 cgggtgaagc gtggaccgcg ggcatggatc tgaaagaata tttccgtgaa gtggatgcgg
     2101 gtccggaaat tctgcaagaa aaaattcgtc gcgaagcgag ccagtggcag tggaaactgc
     2161 tgcgtatgta tgcgaaaccg accattgcca tggtgaacgg ctggtgcttt ggcggcggtt
     2221 ttagcccgct ggttgcgtgc gatctggcca tttgcgcgga tgaagcgacc tttggcctga
     2281 gcgagattaa ctggggcatt ccgccgggta acctggtgag caaagccatg gcggataccg
     2341 tgggccatcg tcagagcctg tattatatca tgaccggcaa aacctttggt ggccagaaag
     2401 cggcggaaat gggcctggtg aacgaaagcg tgccgctggc ccagctgcgt gaagtgacca
     2461 ttgaactggc ccgtaacctg ctggaaaaaa atccggtggt gctgcgtgcg gcgaaacatg
     2521 gctttaaacg ttgccgtgaa ctgacctggg aacagaacga agattatctg tacgcgaaac
     2581 tggatcagag ccgtctgctg gataccgaag gcggccgtga acagggcatg aaacagtttc
     2641 tggatgataa aagcattaaa ccgggcctgc aagcgtataa acgttaataa tactagagcc
     2701 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2761 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2821 gtttata
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