LOCUS       BBa_K243011             1209 bp    DNA     linear       17-May-2024
DEFINITION  Strep-DigA-Long Linker-Fok_i
ACCESSION   BBa_K243011
VERSION     BBa_K243011.1  
FEATURES             Location/Qualifiers
     CDS             1..24
                     /label=Strep-Tag II
     CDS             1..24
                     /label=Strep-Tag II
     misc_feature    1..24
                     /label=BBa_K157012
     misc_feature    25..30
                     /label=Scar 25
     misc_feature    25..30
                     /label=BBa_B0105
     misc_feature    31..582
                     /label=Digoxigenin tag
     misc_feature    31..582
                     /label=BBa_K243003
     misc_feature    583..588
                     /label=Scar 25
     misc_feature    583..588
                     /label=BBa_B0105
     misc_feature    589..624
                     /label=Long Linker
     misc_feature    589..624
                     /label=BBa_K243006
     misc_feature    625..630
                     /label=Scar 25
     misc_feature    625..630
                     /label=BBa_B0105
     CDS             631..1209
                     /label=Fok_i
     misc_feature    631..1209
                     /label=BBa_K243001
ORIGIN
        1 tggagccatc cgcagtttga aaaaaccggc gacgtgtacc acgacggcgc ctgccccgaa
       61 gtgaagcccg tggacaactt cgactggtcc cagtaccacg gcaagtggtg gcaggtggcc
      121 gcttatcccg accacatcac caagtacggc aagtgcggct gggccgagta cacccccgag
      181 ggcaagagcg tgaaggtgtc ccggtacagc gtgatccacg gcaaagagta cttcagcgag
      241 ggcaccgcct accctgtggg cgacagcaag atcggcaaga tctaccacag ctacaccatc
      301 ggcggcgtga cccaggtggg cgtgagcaac gtgctgtcca ccgacaacaa gaactacatc
      361 atcggctact tttgcagata cgacgaggac aagaagggcc actgggacgc cgtgtgggtg
      421 ctgtcccggt ccatggtgct gaccggcgag gccaagaccg ccgtggagaa ctacctgatc
      481 ggcagccccg tggtggacag ccagaaactg gtgtacagcg acttctccga ggccgcctgc
      541 aaagtgaaca acagcaactg gtcccacccc cagttcgaaa agaccggcgg tggttctggt
      601 ggtggttctg gtggtggttc tggtaccggc aaatctgaac tggaggagaa aaaatccgag
      661 ctgcgccaca aactgaaata tgtgcctcac gagtatatcg aactgatcga gatcgcccgt
      721 aatagtaccc aagaccgtat cctggaaatg aaagtgatgg agttcttcat gaaagtctat
      781 ggctatcgtg gcaaacatct gggtggtagc cgtaaaccag caggtgccat ttataccgtt
      841 ggttccccga tcgattatgg cgttatcgtg gccacaaaag cgtattctgg cggttataat
      901 ctgccgattg gtcaggctga tgagatggaa cgttatgtgg aagagaatca gacccgtaac
      961 aaacatctga acccgaacga atggtggaaa gtgtatccgt caagtgtcac cgagttcaaa
     1021 tttctgttcg tgagcggcca ctttaaaggc aactataaag cccagctgac tcgtctgaac
     1081 catatcacca atagcaatgg ggcagtgctg agtgttgagg aactgctgat cggtggagaa
     1141 atgatcaaag caggcaccct gactctggaa gaagttcgcc gtaaattcaa caatggcgag
     1201 atcaatttt
//