LOCUS       BBa_K243021             1272 bp    DNA     linear       10-May-2024
DEFINITION  DsbA-His-DigA-Split Linker-Fok_a
ACCESSION   BBa_K243021
VERSION     BBa_K243021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..54
                     /label=Dsba signal sequence
     misc_feature    1..54
                     /label=BBa_K243002
     misc_feature    55..60
                     /label=Scar 25
     misc_feature    55..60
                     /label=BBa_B0105
     CDS             61..78
                     /label=His-Tag
     misc_feature    61..78
                     /label=BBa_K157011
     misc_feature    79..84
                     /label=Scar 25
     misc_feature    79..84
                     /label=BBa_B0105
     misc_feature    85..636
                     /label=Digoxigenin tag
     misc_feature    85..636
                     /label=BBa_K243003
     misc_feature    637..642
                     /label=Scar 25
     misc_feature    637..642
                     /label=BBa_B0105
     CDS             643..693
                     /label=Split-Fluorophore Linker
     misc_feature    643..693
                     /label=BBa_K157009
     CDS             694..1272
                     /label=Fok_a
     misc_feature    694..1272
                     /label=BBa_K243000
ORIGIN
        1 aaaaagattt ggctggcgct ggctggttta gttttagcgt ttagcgcatc ggcgaccggc
       61 catcatcatc atcatcatac cggcgacgtg taccacgacg gcgcctgccc cgaagtgaag
      121 cccgtggaca acttcgactg gtcccagtac cacggcaagt ggtggcaggt ggccgcttat
      181 cccgaccaca tcaccaagta cggcaagtgc ggctgggccg agtacacccc cgagggcaag
      241 agcgtgaagg tgtcccggta cagcgtgatc cacggcaaag agtacttcag cgagggcacc
      301 gcctaccctg tgggcgacag caagatcggc aagatctacc acagctacac catcggcggc
      361 gtgacccagg tgggcgtgag caacgtgctg tccaccgaca acaagaacta catcatcggc
      421 tacttttgca gatacgacga ggacaagaag ggccactggg acgccgtgtg ggtgctgtcc
      481 cggtccatgg tgctgaccgg cgaggccaag accgccgtgg agaactacct gatcggcagc
      541 cccgtggtgg acagccagaa actggtgtac agcgacttct ccgaggccgc ctgcaaagtg
      601 aacaacagca actggtccca cccccagttc gaaaagaccg gccgaccagc ctgtaagatt
      661 ccaaatgacc tgaagcagaa agttatgaat cacaaatctg aactggagga gaaaaaatcc
      721 gagctgcgcc acaaactgaa atatgtgcct cacgagtata tcgaactgat cgagatcgcc
      781 cgtaatagta cccaagaccg tatcctggaa atgaaagtga tggagttctt catgaaagtc
      841 tatggctatc gtggcaaaca tctgggtggt agccgtaaac ctgatggtgc catttatacc
      901 gttggttccc cgatcgatta tggcgttatc gttgatacca aagcctatag cgggggttat
      961 aacctgccaa ttggtcaggc tgatgagatg cagcgttatg tgaaagagaa ccagactcgt
     1021 aacaaacaca tcaacccgaa cgaatggtgg aaagtgtatc cgtcaagcgt tacagagttc
     1081 aaattcctgt tcgtgagcgg ccattttaaa ggcaactata aagcacagct gacccgtctg
     1141 aaccataaaa ccaatagcaa tggcgccgtt ctgtcagtag aagagctgct gattggcggt
     1201 gaaatgatca aagccgggac cctgacactg gaagaagttc gccgtaaatt caacaatggg
     1261 gagatcaatt tt
//