LOCUS       BBa_K248003             1159 bp    DNA     linear       24-May-2024
DEFINITION  pNirK-RBS-TetR
ACCESSION   BBa_K248003
VERSION     BBa_K248003.1  
FEATURES             Location/Qualifiers
     promoter        1..312
                     /label=nirK
     promoter        1..312
                     /label=BBa_K248002
     RBS             321..331
                     /label=RBS-4\Weaker
     RBS             327..330
                     /label=RBS
     misc_feature    321..331
                     /label=BBa_B0033
     RBS             321..331
                     /label=BBa_B0033
     CDS             341..957
                     /label=tetR
     misc_feature    958..991
                     /label=SsrA
     misc_feature    998..1022
                     /label=Help:Barcodes
     CDS             338..997
                     /label=BBa_C0040
     stem_loop       1042..1085
                     /label=stem_loop
     misc_feature    1031..1110
                     /label=BBa_B0010
     terminator      1031..1110
                     /label=BBa_B0010
     stem_loop       1126..1145
                     /label=T7 TE
     misc_feature    1152..1152
                     /label=stop
     polyA_site      1146..1159
                     /label=polya
     misc_feature    1119..1159
                     /label=BBa_B0012
     terminator      1119..1159
                     /label=BBa_B0012
     misc_feature    321..1159
                     /label=BBa_P0340
ORIGIN
        1 gttgcgcgaa ataccgtaac aatccgcaat ttccgtaatg gtggacaatt cctctctttt
       61 caaaccgaga taagtgagga tgcgtaacgc gtaatcgctg taattcgtca gtctcatgaa
      121 gtattaccca tttattgagt gggggttaac aagtatatcc ccactttttt atttctgccc
      181 gactggttga caatctgaat cgaacgggta aaatccatca aaagatatat taataatata
      241 tctttaagag gaggtggtta atgatcaccg aagaagttaa atatgactgt atttaaaggt
      301 ctttcgtgca gatactagag tcacacagga ctactagatg tccagattag ataaaagtaa
      361 agtgattaac agcgcattag agctgcttaa tgaggtcgga atcgaaggtt taacaacccg
      421 taaactcgcc cagaagctag gtgtagagca gcctacattg tattggcatg taaaaaataa
      481 gcgggctttg ctcgacgcct tagccattga gatgttagat aggcaccata ctcacttttg
      541 ccctttagaa ggggaaagct ggcaagattt tttacgtaat aacgctaaaa gttttagatg
      601 tgctttacta agtcatcgcg atggagcaaa agtacattta ggtacacggc ctacagaaaa
      661 acagtatgaa actctcgaaa atcaattagc ctttttatgc caacaaggtt tttcactaga
      721 gaatgcatta tatgcactca gcgctgtggg gcattttact ttaggttgcg tattggaaga
      781 tcaagagcat caagtcgcta aagaagaaag ggaaacacct actactgata gtatgccgcc
      841 attattacga caagctatcg aattatttga tcaccaaggt gcagagccag ccttcttatt
      901 cggccttgaa ttgatcatat gcggattaga aaaacaactt aaatgtgaaa gtgggtccgc
      961 tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct agtgtagatc
     1021 actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1081 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1141 gggcctttct gcgtttata
//