LOCUS       BBa_K255003             3038 bp    DNA     linear       01-Jun-2024
DEFINITION  pLac.lacI-cfp(fusion).terminator.pTet.yfp.terminator
ACCESSION   BBa_K255003
VERSION     BBa_K255003.1  
FEATURES             Location/Qualifiers
     misc_feature    3..19
                     /label=lac O1
     promoter        20..25
                     /label=-35
     misc_feature    26..42
                     /label=lac O1
     promoter        43..48
                     /label=-10
     misc_feature    1..54
                     /label=BBa_R0011
     promoter        1..54
                     /label=BBa_R0011
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     CDS             82..1953
                     /label=BBa_K255000
     stem_loop       1973..2016
                     /label=stem_loop
     misc_feature    1962..2041
                     /label=BBa_B0010
     terminator      1962..2041
                     /label=BBa_B0010
     stem_loop       2057..2076
                     /label=T7 TE
     misc_feature    2083..2083
                     /label=stop
     polyA_site      2077..2090
                     /label=polya
     misc_feature    2050..2090
                     /label=BBa_B0012
     terminator      2050..2090
                     /label=BBa_B0012
     misc_feature    2099..2117
                     /label=TetR 1
     misc_feature    2099..2152
                     /label=BBa_R0040
     promoter        2118..2123
                     /label=-35
     misc_feature    2124..2142
                     /label=TetR 2
     promoter        2141..2146
                     /label=-10
     promoter        2099..2152
                     /label=BBa_R0040
     misc_feature    2165..2168
                     /label=conserved
     RBS             2161..2172
                     /label=BBa_B0034
     CDS             2179..2901
                     /label=BBa_E0030
     stem_loop       2921..2964
                     /label=stem_loop
     misc_feature    2910..2989
                     /label=BBa_B0010
     terminator      2910..2989
                     /label=BBa_B0010
     stem_loop       3005..3024
                     /label=T7 TE
     misc_feature    3031..3031
                     /label=stop
     polyA_site      3025..3038
                     /label=polya
     misc_feature    2998..3038
                     /label=BBa_B0012
     terminator      2998..3038
                     /label=BBa_B0012
ORIGIN
        1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta
       61 gagaaagagg agaaatacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc
      121 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
      181 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
      241 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
      301 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
      361 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
      421 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
      481 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
      541 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
      601 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
      661 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
      721 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
      781 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
      841 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
      901 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
      961 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     1021 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     1081 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
     1141 tcccgactgg aaagcgggca gctcgaggga gaaggacaag gacaaggaca aggaccagga
     1201 cgaggatacg cataccgaag cggttccgga atggtgagca agggcgagga gctgttcacc
     1261 ggggtggtgc ccatcctggt cgagctggac ggcgacgtga acggccacaa gttcagcgtg
     1321 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     1381 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgacctg gggcgtgcag
     1441 tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     1501 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     1561 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     1621 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacat cagccacaac
     1681 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccgccac
     1741 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     1801 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa
     1861 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1921 actctcggca tggacgagct gtacaagtaa taatactaga gccaggcatc aaataaaacg
     1981 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     2041 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtc
     2101 cctatcagtg atagagattg acatccctat cagtgataga gatactgagc actactagag
     2161 aaagaggaga aatactagat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     2221 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     2281 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     2341 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcaatg cttcgcccgc
     2401 taccccgacc acatgaagct gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     2461 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     2521 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     2581 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     2641 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     2701 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     2761 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag
     2821 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     2881 gacgagctgt acaagtaata atactagagc caggcatcaa ataaaacgaa aggctcagtc
     2941 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     3001 cactggctca ccttcgggtg ggcctttctg cgtttata
//