LOCUS       BBa_K255004             3037 bp    DNA     linear       14-Jun-2024
DEFINITION  pTet.lacI-cfp(fusion).terminator.pTet.yfp.terminator
ACCESSION   BBa_K255004
VERSION     BBa_K255004.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..1952
                     /label=BBa_K255000
     stem_loop       1972..2015
                     /label=stem_loop
     misc_feature    1961..2040
                     /label=BBa_B0010
     terminator      1961..2040
                     /label=BBa_B0010
     stem_loop       2056..2075
                     /label=T7 TE
     misc_feature    2082..2082
                     /label=stop
     polyA_site      2076..2089
                     /label=polya
     misc_feature    2049..2089
                     /label=BBa_B0012
     terminator      2049..2089
                     /label=BBa_B0012
     misc_feature    2098..2116
                     /label=TetR 1
     misc_feature    2098..2151
                     /label=BBa_R0040
     promoter        2117..2122
                     /label=-35
     misc_feature    2123..2141
                     /label=TetR 2
     promoter        2140..2145
                     /label=-10
     promoter        2098..2151
                     /label=BBa_R0040
     misc_feature    2164..2167
                     /label=conserved
     RBS             2160..2171
                     /label=BBa_B0034
     CDS             2178..2900
                     /label=BBa_E0030
     stem_loop       2920..2963
                     /label=stem_loop
     misc_feature    2909..2988
                     /label=BBa_B0010
     terminator      2909..2988
                     /label=BBa_B0010
     stem_loop       3004..3023
                     /label=T7 TE
     misc_feature    3030..3030
                     /label=stop
     polyA_site      3024..3037
                     /label=polya
     misc_feature    2997..3037
                     /label=BBa_B0012
     terminator      2997..3037
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaccag taacgttata cgatgtcgca gagtatgccg
      121 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
      181 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
      241 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
      301 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
      361 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
      421 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
      481 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
      541 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
      601 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
      661 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
      721 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
      781 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
      841 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg
      901 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
      961 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     1021 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     1081 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     1141 cccgactgga aagcgggcag ctcgagggag aaggacaagg acaaggacaa ggaccaggac
     1201 gaggatacgc ataccgaagc ggttccggaa tggtgagcaa gggcgaggag ctgttcaccg
     1261 gggtggtgcc catcctggtc gagctggacg gcgacgtgaa cggccacaag ttcagcgtgt
     1321 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
     1381 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt
     1441 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     1501 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
     1561 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     1621 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacatc agccacaacg
     1681 tctatatcac cgccgacaag cagaagaacg gcatcaaggc caacttcaag atccgccaca
     1741 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1801 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1861 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1921 ctctcggcat ggacgagctg tacaagtaat aatactagag ccaggcatca aataaaacga
     1981 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2041 tactagagtc acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc
     2101 ctatcagtga tagagattga catccctatc agtgatagag atactgagca ctactagaga
     2161 aagaggagaa atactagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     2221 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     2281 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     2341 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgcaatgc ttcgcccgct
     2401 accccgacca catgaagctg cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     2461 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     2521 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     2581 gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg
     2641 ccgacaagca gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg
     2701 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     2761 tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac cccaacgaga
     2821 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     2881 acgagctgta caagtaataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     2941 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3001 actggctcac cttcgggtgg gcctttctgc gtttata
//