LOCUS       BBa_K256022             2081 bp    DNA     linear       09-May-2024
DEFINITION  J23119:IFP:HO1
ACCESSION   BBa_K256022
VERSION     BBa_K256022.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    62..1030
                     /label=misc
     CDS             62..1030
                     /label=BBa_K256008
     stem_loop       1050..1093
                     /label=stem_loop
     misc_feature    1039..1118
                     /label=BBa_B0010
     terminator      1039..1118
                     /label=BBa_B0010
     stem_loop       1134..1153
                     /label=T7 TE
     misc_feature    1160..1160
                     /label=stop
     polyA_site      1154..1167
                     /label=polya
     misc_feature    1127..1167
                     /label=BBa_B0012
     terminator      1127..1167
                     /label=BBa_B0012
     misc_feature    62..1167
                     /label=BBa_K256009
     misc_feature    1180..1183
                     /label=conserved
     RBS             1176..1187
                     /label=BBa_B0034
     misc_feature    1920..1944
                     /label=Help:Barcodes
     CDS             1194..1944
                     /label=BBa_I15008
     stem_loop       1964..2007
                     /label=stem_loop
     misc_feature    1953..2032
                     /label=BBa_B0010
     terminator      1953..2032
                     /label=BBa_B0010
     stem_loop       2048..2067
                     /label=T7 TE
     misc_feature    2074..2074
                     /label=stop
     polyA_site      2068..2081
                     /label=polya
     misc_feature    2041..2081
                     /label=BBa_B0012
     terminator      2041..2081
                     /label=BBa_B0012
     terminator      1953..2081
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatggcacgt gatccgctgc cgttttttcc tccgctgtat ctgggtggtc cggaaattac
      121 caccgaaaat tgtgaacgcg aaccgattca tattccgggt agcattcagc cgcatggtgc
      181 actgctgacc gcagatggtc atagcggtga agttctgcaa gttagcctga atgcagcaac
      241 ctttctgggt caggaaccga ccgttctgcg cggtcagacc ctggccgcac tgctgccgga
      301 acagtggcct gcactgcaag cagctctgcc tccgggttgt ccggatgcac tgcaatatcg
      361 tgcaaccctg gattggccag cagcaggtca tctgagcctg accgttcatc gtgttgcaga
      421 actgctgatt ctggaatttg aaccgaccga agcatgggat agcattggtc cgcatgcgct
      481 gcgtaatgca atgtttgcac tggaaagcgc accgaatctg cgtgcactgg cagaagttgc
      541 aacccagacc gttcgtgaac tgaccggttt tgatcgtgtg atgctgtata aatttgcccc
      601 ggatgcaacc ggtgaaatga ttgcagaagc acgtcgcgaa ggtatgcagg catttctggg
      661 tcatcgtttt ccggcaagcc atacaccggc acaggcccgt gcactgtata cccgtcatct
      721 gctgcgtctg accgctgata cccgtgcagc agcagttccg ctggatccgg ttctgaatcc
      781 gcagaccaat gcaccgacac cgctgggtgg tgcagttctg cgtgcaacca gcccgatgca
      841 tatgcagtat ctgcgtaata tgggtgttgg tagcagcctg agcgttagcg ttgttgttgg
      901 tggtcagctg tggggtctga ttgtttgtca tcatcagacc ccgtatgttc tgccgccgga
      961 tctgcgtacc accctggaag aactgggtcg taaactgagc ggtcaggttc agcgtaaaga
     1021 agcataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1081 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1141 cttcgggtgg gcctttctgc gtttatatac tagagaaaga ggagaaatac tagatgagtg
     1201 tcaacttagc ttcccagttg cgggaaggga cgaaaaaatc ccactccatg gcggagaacg
     1261 tcggctttgt caaatgcttc ctcaagggcg ttgtcgagaa aaattcctac cgtaagctgg
     1321 ttggcaatct ctactttgtc tacagtgcca tggaagagga aatggcaaaa tttaaggacc
     1381 atcccatcct cagccacatt tacttccccg aactcaaccg caaacaaagc ctagagcaag
     1441 acctgcaatt ctattacggc tccaactggc ggcaagaagt gaaaatttct gccgctggcc
     1501 aagcctatgt ggaccgagtc cggcaagtgg ccgctacggc ccctgaattg ttggtggccc
     1561 attcctacac ccgttacctg ggggatcttt ccggcggtca aattctcaag aaaattgccc
     1621 aaaatgccat gaatctccac gatggtggca cagctttcta tgaatttgcc gacattgatg
     1681 acgaaaaggc ttttaaaaat acctaccgtc aagctatgaa tgatctgccc attgaccaag
     1741 ccaccgccga acggattgtg gatgaagcca atgacgcctt tgccatgaac atgaaaatgt
     1801 tcaacgaact tgaaggcaac ctgatcaagg cgatcggcat tatggtgttc aacagcctca
     1861 cccgtcgccg cagtcaaggc agcaccgaag ttggcctcgc cacctccgaa ggctaataac
     1921 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
     1981 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2041 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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