LOCUS       BBa_K256027             1843 bp    DNA     linear       09-May-2024
DEFINITION  B0034:CHE:B0034:GFP:B0015
ACCESSION   BBa_K256027
VERSION     BBa_K256027.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..960
                     /label=misc
     CDS             19..960
                     /label=BBa_K256023
     misc_feature    973..976
                     /label=conserved
     RBS             969..980
                     /label=BBa_B0034
     CDS             987..1706
                     /label=GFP protein
     CDS             987..1706
                     /label=BBa_E0040
     stem_loop       1726..1769
                     /label=stem_loop
     misc_feature    1715..1794
                     /label=BBa_B0010
     terminator      1715..1794
                     /label=BBa_B0010
     stem_loop       1810..1829
                     /label=T7 TE
     misc_feature    1836..1836
                     /label=stop
     polyA_site      1830..1843
                     /label=polya
     misc_feature    1803..1843
                     /label=BBa_B0012
     terminator      1803..1843
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaaaaaaaaa agcctgctgc cgctgggtct ggcaattggt
       61 ctggcaagcc tggcagcatc tccgctgatt caggcaagca cctataccca gaccaaatat
      121 ccgattgttc tggcacatgg tatgctgggc tttgataata ttctgggcgt ggattattgg
      181 tttggtattc cgagcgcact gcgtcgtgat ggtgcacagg tttatgttac cgaagttagc
      241 cagctggata ccagcgaagt tcgtggtgaa cagctgctgc aacaggttga agaaattgtt
      301 gcactgagcg gtcagccgaa agttaatctg attggtcata gccatggtgg tccgaccatt
      361 cgttatgttg cagcagttcg tccggatctg attgcaagcg caaccagcgt tggtgcaccg
      421 cataaaggta gcgataccgc agattttctg cgtcagattc ctccgggtag cgcaggcgaa
      481 gcaattctga gcggtctggt taatagcctg ggtgcactga ttagctttct gagcagcggt
      541 agcaccggca cccagaatag tctgggttct ctggaaagcc tgaatagcga aggtgcagca
      601 cgttttaatg caaaatatcc gcagggtgtt ccgaccagcg catgtggtga aggtgcctat
      661 aaagttaatg gcgtgagcta ttatagctgg tctggtagct ctccgctgac caattttctg
      721 gatccgtctg atgcatttct gggtgcaagc agcctgacct ttaaaaatgg caccgcaaat
      781 gatggtctgg ttggcacctg tagcagccat ctgggtatgg ttattcgcga taattaccgc
      841 atgaatcatc tggatgaagt gaatcaggtt tttggtctga ccagcctgtt tgaaacctct
      901 ccggttagcg tttatcgtca gcatgcaaat cgtctgaaaa atgccagcct gtaataataa
      961 tactagagaa agaggagaaa tactagatgc gtaaaggaga agaacttttc actggagttg
     1021 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     1081 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     1141 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
     1201 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
     1261 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     1321 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     1381 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
     1441 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
     1501 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     1561 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     1621 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     1681 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct
     1741 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1801 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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