LOCUS       BBa_K256028             1305 bp    DNA     linear       09-May-2024
DEFINITION  placI:CHE
ACCESSION   BBa_K256028
VERSION     BBa_K256028.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..1168
                     /label=misc
     CDS             227..1168
                     /label=BBa_K256023
     stem_loop       1188..1231
                     /label=stem_loop
     misc_feature    1177..1256
                     /label=BBa_B0010
     terminator      1177..1256
                     /label=BBa_B0010
     stem_loop       1272..1291
                     /label=T7 TE
     misc_feature    1298..1298
                     /label=stop
     polyA_site      1292..1305
                     /label=polya
     misc_feature    1265..1305
                     /label=BBa_B0012
     terminator      1265..1305
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaaaaaaaag
      241 cctgctgccg ctgggtctgg caattggtct ggcaagcctg gcagcatctc cgctgattca
      301 ggcaagcacc tatacccaga ccaaatatcc gattgttctg gcacatggta tgctgggctt
      361 tgataatatt ctgggcgtgg attattggtt tggtattccg agcgcactgc gtcgtgatgg
      421 tgcacaggtt tatgttaccg aagttagcca gctggatacc agcgaagttc gtggtgaaca
      481 gctgctgcaa caggttgaag aaattgttgc actgagcggt cagccgaaag ttaatctgat
      541 tggtcatagc catggtggtc cgaccattcg ttatgttgca gcagttcgtc cggatctgat
      601 tgcaagcgca accagcgttg gtgcaccgca taaaggtagc gataccgcag attttctgcg
      661 tcagattcct ccgggtagcg caggcgaagc aattctgagc ggtctggtta atagcctggg
      721 tgcactgatt agctttctga gcagcggtag caccggcacc cagaatagtc tgggttctct
      781 ggaaagcctg aatagcgaag gtgcagcacg ttttaatgca aaatatccgc agggtgttcc
      841 gaccagcgca tgtggtgaag gtgcctataa agttaatggc gtgagctatt atagctggtc
      901 tggtagctct ccgctgacca attttctgga tccgtctgat gcatttctgg gtgcaagcag
      961 cctgaccttt aaaaatggca ccgcaaatga tggtctggtt ggcacctgta gcagccatct
     1021 gggtatggtt attcgcgata attaccgcat gaatcatctg gatgaagtga atcaggtttt
     1081 tggtctgacc agcctgtttg aaacctctcc ggttagcgtt tatcgtcagc atgcaaatcg
     1141 tctgaaaaat gccagcctgt aataataata ctagagccag gcatcaaata aaacgaaagg
     1201 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact
     1261 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//