LOCUS       BBa_K256032             2149 bp    DNA     linear       09-May-2024
DEFINITION  placiq:luxR:pluxR:CHE
ACCESSION   BBa_K256032
VERSION     BBa_K256032.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    64..65
                     /label=prefix
     misc_feature    237..237
                     /label=T
     misc_feature    555..555
                     /label=A
     CDS             64..813
                     /label=luxR
     misc_feature    64..819
                     /label=BBa_C0062
     misc_feature    820..844
                     /label=Help:Barcodes
     CDS             64..819
                     /label=BBa_C0062
     stem_loop       864..907
                     /label=stem_loop
     misc_feature    853..932
                     /label=BBa_B0010
     terminator      853..932
                     /label=BBa_B0010
     stem_loop       948..967
                     /label=T7 TE
     misc_feature    974..974
                     /label=stop
     polyA_site      968..981
                     /label=polya
     misc_feature    941..981
                     /label=BBa_B0012
     terminator      941..981
                     /label=BBa_B0012
     terminator      853..981
                     /label=BBa_B0015
     misc_feature    990..1009
                     /label=LuxR/HSL
     promoter        1009..1014
                     /label=-35
     promoter        1031..1036
                     /label=-10
     misc_feature    1042..1042
                     /label=start
     misc_feature    990..1044
                     /label=BBa_R0062
     promoter        990..1044
                     /label=BBa_R0062
     misc_feature    1057..1060
                     /label=conserved
     RBS             1053..1064
                     /label=BBa_B0034
     misc_feature    1071..2012
                     /label=misc
     CDS             1071..2012
                     /label=BBa_K256023
     stem_loop       2032..2075
                     /label=stem_loop
     misc_feature    2021..2100
                     /label=BBa_B0010
     terminator      2021..2100
                     /label=BBa_B0010
     stem_loop       2116..2135
                     /label=T7 TE
     misc_feature    2142..2142
                     /label=stop
     polyA_site      2136..2149
                     /label=polya
     misc_feature    2109..2149
                     /label=BBa_B0012
     terminator      2109..2149
                     /label=BBa_B0012
     terminator      2021..2149
                     /label=BBa_B0015
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatgaaaa acataaatgc cgacgacaca tacagaataa ttaataaaat taaagcttgt
      121 agaagcaata atgatattaa tcaatgctta tctgatatga ctaaaatggt acattgtgaa
      181 tattatttac tcgcgatcat ttatcctcat tctatggtta aatctgatat ttcaatccta
      241 gataattacc ctaaaaaatg gaggcaatat tatgatgacg ctaatttaat aaaatatgat
      301 cctatagtag attattctaa ctccaatcat tcaccaatta attggaatat atttgaaaac
      361 aatgctgtaa ataaaaaatc tccaaatgta attaaagaag cgaaaacatc aggtcttatc
      421 actgggttta gtttccctat tcatacggct aacaatggct tcggaatgct tagttttgca
      481 cattcagaaa aagacaacta tatagatagt ttatttttac atgcgtgtat gaacatacca
      541 ttaattgttc cttctctagt tgataattat cgaaaaataa atatagcaaa taataaatca
      601 aacaacgatt taaccaaaag agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc
      661 tcttgggata tttcaaaaat attaggttgc agtgagcgta ctgtcacttt ccatttaacc
      721 aatgcgcaaa tgaaactcaa tacaacaaac cgctgccaaa gtatttctaa agcaatttta
      781 acaggagcaa ttgattgccc atactttaaa aattaataac actgatagtg ctagtgtaga
      841 tcactactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      901 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      961 gtgggccttt ctgcgtttat atactagaga cctgtaggat cgtacaggtt tacgcaagaa
     1021 aatggtttgt tatagtcgaa taaatactag agaaagagga gaaatactag atgaaaaaaa
     1081 aaagcctgct gccgctgggt ctggcaattg gtctggcaag cctggcagca tctccgctga
     1141 ttcaggcaag cacctatacc cagaccaaat atccgattgt tctggcacat ggtatgctgg
     1201 gctttgataa tattctgggc gtggattatt ggtttggtat tccgagcgca ctgcgtcgtg
     1261 atggtgcaca ggtttatgtt accgaagtta gccagctgga taccagcgaa gttcgtggtg
     1321 aacagctgct gcaacaggtt gaagaaattg ttgcactgag cggtcagccg aaagttaatc
     1381 tgattggtca tagccatggt ggtccgacca ttcgttatgt tgcagcagtt cgtccggatc
     1441 tgattgcaag cgcaaccagc gttggtgcac cgcataaagg tagcgatacc gcagattttc
     1501 tgcgtcagat tcctccgggt agcgcaggcg aagcaattct gagcggtctg gttaatagcc
     1561 tgggtgcact gattagcttt ctgagcagcg gtagcaccgg cacccagaat agtctgggtt
     1621 ctctggaaag cctgaatagc gaaggtgcag cacgttttaa tgcaaaatat ccgcagggtg
     1681 ttccgaccag cgcatgtggt gaaggtgcct ataaagttaa tggcgtgagc tattatagct
     1741 ggtctggtag ctctccgctg accaattttc tggatccgtc tgatgcattt ctgggtgcaa
     1801 gcagcctgac ctttaaaaat ggcaccgcaa atgatggtct ggttggcacc tgtagcagcc
     1861 atctgggtat ggttattcgc gataattacc gcatgaatca tctggatgaa gtgaatcagg
     1921 tttttggtct gaccagcctg tttgaaacct ctccggttag cgtttatcgt cagcatgcaa
     1981 atcgtctgaa aaatgccagc ctgtaataat aatactagag ccaggcatca aataaaacga
     2041 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     2101 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//