LOCUS       BBa_K257011             2838 bp    DNA     linear       01-Jun-2024
DEFINITION  PBad + clyA_CTerm + RFP_NTerm
ACCESSION   BBa_K257011
VERSION     BBa_K257011.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1226
                     /label=Good rbs
     CDS             1231..2139
                     /label=hlyE
     misc_feature    2140..2154
                     /label=poly Gly linker
     misc_feature    1219..2155
                     /label=BBa_K257002
     CDS             2161..2832
                     /label=RFP
     misc_feature    2833..2838
                     /label=double codon stop
     misc_feature    2156..2838
                     /label=BBa_K257008
     misc_feature    1219..2838
                     /label=BBa_K257010
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagag gaggtatata atgactgaaa tcgttgcaga taaaacggta
     1261 gaagtagtta aaaacgcaat cgaaaccgca gatggagcat tagatcttta taataaatat
     1321 ctcgatcagg tcatcccctg gcagaccttt gatgaaacca taaaagagtt aagtcgcttt
     1381 aaacaggagt attcacaggc agcctccgtt ttagtcggcg atattaaaac cttacttatg
     1441 gatagccagg ataagtattt tgaagcaacc caaacagtgt atgaatggtg tggtgttgcg
     1501 acgcaattgc tcgcagcgta tattttgcta tttgatgagt acaatgagaa gaaagcatcc
     1561 gcccagaaag acattctcat taaggtactg gatgacggca tcacgaagct gaatgaagcg
     1621 caaaaatccc tgctggtaag ctcacaaagt ttcaacaacg cttccgggaa actgctggcg
     1681 ttagatagcc agttaaccaa tgatttttca gaaaaaagca gctatttcca gtcacaggta
     1741 gataaaatca ggaaggaagc atatgccggt gccgcagccg gtgtcgtcgc cggtccattt
     1801 ggattaatca tttcctattc tattgctgcg ggcgtagttg aaggaaaact gattccagaa
     1861 ttgaagaaca agttaaaatc tgtgcagaat ttctttacca ccctgtctaa cacggttaaa
     1921 caagcgaata aagatatcga tgccgccaaa ttgaaattaa ccaccgaaat agccgccatc
     1981 ggtgagataa aaacggaaac tgaaacaacc agattctacg ttgattatga tgatttaatg
     2041 ctttctttgc taaaagaagc ggccaaaaaa atgattaaca cctgtaatga gtatcagaaa
     2101 agacacggta aaaagacact ctttgaggta cctgaagtcg gcggcggcgg cggcactaga
     2161 gcttcctccg aagacgttat caaagagttc atgcgtttca aagttcgtat ggaaggttcc
     2221 gttaacggtc acgagttcga aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc
     2281 cagaccgcta aactgaaagt taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg
     2341 tccccgcagt tccagtacgg ttccaaagct tacgttaaac acccggctga catcccggac
     2401 tacctgaaac tgtccttccc ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac
     2461 ggtggtgttg ttaccgttac ccaggactcc tccctgcaag acggtgagtt catctacaaa
     2521 gttaaactgc gtggtaccaa cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg
     2581 ggttgggaag cttccaccga acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc
     2641 aaaatgcgtc tgaaactgaa agacggtggt cactacgacg ctgaagttaa aaccacctac
     2701 atggctaaaa aaccggttca gctgccgggt gcttacaaaa ccgacatcaa actggacatc
     2761 acctcccaca acgaagacta caccatcgtt gaacagtacg aacgtgctga aggtcgtcac
     2821 tccaccggtg cttaataa
//