LOCUS       BBa_K257012             2491 bp    DNA     linear       01-Jun-2024
DEFINITION  PBad + RBS_LacI_LVA + Ter
ACCESSION   BBa_K257012
VERSION     BBa_K257012.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     RBS             1219..1229
                     /label=RBS-4\Weaker
     RBS             1225..1228
                     /label=RBS
     misc_feature    1219..1229
                     /label=BBa_B0033
     RBS             1219..1229
                     /label=BBa_B0033
     misc_feature    2325..2363
                     /label=LVA
     CDS             1236..2363
                     /label=lacI-LVA
     misc_feature    1236..2363
                     /label=BBa_C0012
     misc_feature    2364..2388
                     /label=Help:Barcodes
     CDS             1236..2363
                     /label=BBa_C0012
     misc_feature    1219..2388
                     /label=BBa_J24679
     stem_loop       2404..2423
                     /label=T7 TE
     misc_feature    2430..2430
                     /label=stop
     polyA_site      2424..2437
                     /label=polya
     misc_feature    2397..2437
                     /label=BBa_B0012
     terminator      2397..2437
                     /label=BBa_B0012
     stem_loop       2458..2480
                     /label=stem_loop
     misc_feature    2446..2491
                     /label=BBa_B0011
     terminator      2446..2491
                     /label=BBa_B0011
     terminator      2397..2491
                     /label=BBa_B0014
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagtc acacaggact actagatggt gaatgtgaaa ccagtaacgt
     1261 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     1321 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     1381 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     1441 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     1501 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     1561 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     1621 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     1681 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     1741 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     1801 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     1861 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     1921 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     1981 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     2041 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     2101 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     2161 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     2221 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2281 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcaggctgca aacgacgaaa
     2341 actacgcttt agtagcttaa taactctgat agtgctagtg tagatctcta ctagagtcac
     2401 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag
     2461 ccagattatt aatccggctt ttttattatt t
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