LOCUS       BBa_K257021             1646 bp    DNA     linear       09-May-2024
DEFINITION  PLacI + RBS-TetR + Ter + Ptet + RFP
ACCESSION   BBa_K257021
VERSION     BBa_K257021.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     RBS             46..58
                     /label=RBS-3\Weak
     RBS             52..55
                     /label=RBS
     misc_feature    46..58
                     /label=BBa_B0032
     RBS             46..58
                     /label=BBa_B0032
     CDS             68..684
                     /label=tetR
     misc_feature    685..718
                     /label=SsrA
     misc_feature    725..749
                     /label=Help:Barcodes
     CDS             65..749
                     /label=BBa_C0040
     misc_feature    46..749
                     /label=BBa_K136050
     stem_loop       765..784
                     /label=T7 TE
     misc_feature    791..791
                     /label=stop
     polyA_site      785..798
                     /label=polya
     misc_feature    758..798
                     /label=BBa_B0012
     terminator      758..798
                     /label=BBa_B0012
     stem_loop       819..841
                     /label=stem_loop
     misc_feature    807..852
                     /label=BBa_B0011
     terminator      807..852
                     /label=BBa_B0011
     terminator      758..852
                     /label=BBa_B0014
     misc_feature    1..852
                     /label=BBa_K257015
     misc_feature    861..879
                     /label=TetR 1
     misc_feature    861..914
                     /label=BBa_R0040
     promoter        880..885
                     /label=-35
     misc_feature    886..904
                     /label=TetR 2
     promoter        903..908
                     /label=-10
     promoter        861..914
                     /label=BBa_R0040
     misc_feature    927..930
                     /label=conserved
     RBS             923..934
                     /label=BBa_B0034
     CDS             941..1615
                     /label=mrfp1
     misc_feature    1622..1646
                     /label=Help:Barcodes
     CDS             941..1646
                     /label=BBa_E1010
     misc_feature    923..1646
                     /label=BBa_K093005
     misc_feature    861..1646
                     /label=BBa_K257014
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagtcaca caggaaagta
       61 ctagatgtcc agattagata aaagtaaagt gattaacagc gcattagagc tgcttaatga
      121 ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg tagagcagcc
      181 tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag ccattgagat
      241 gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc aagatttttt
      301 acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg gagcaaaagt
      361 acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc aattagcctt
      421 tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg ctgtggggca
      481 ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag aagaaaggga
      541 aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat tatttgatca
      601 ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg gattagaaaa
      661 acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt tagtagctta
      721 ataacactga tagtgctagt gtagatcact actagagtca cactggctca ccttcgggtg
      781 ggcctttctg cgtttatata ctagagagag aatataaaaa gccagattat taatccggct
      841 tttttattat tttactagag tccctatcag tgatagagat tgacatccct atcagtgata
      901 gagatactga gcactactag agaaagagga gaaatactag atggcttcct ccgaagacgt
      961 tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt
     1021 cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa
     1081 agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta
     1141 cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt
     1201 cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt
     1261 tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac
     1321 caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac
     1381 cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact
     1441 gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt
     1501 tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga
     1561 ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata
     1621 acgctgatag tgctagtgta gatcgc
//