LOCUS       BBa_K257023             3044 bp    DNA     linear       09-May-2024
DEFINITION  fec promoter + RFP
ACCESSION   BBa_K257023
VERSION     BBa_K257023.1  
FEATURES             Location/Qualifiers
     misc_feature    1..2934
                     /label=BBa_J61002
     RBS             1..12
                     /label=RBS
     misc_feature    1..12
                     /label=BBa_B0034 RBS
     CDS             19..699
                     /label=mRFP
     misc_feature    733..861
                     /label=BBa_B0015
     stem_loop       744..787
                     /label=rrnB terminator
     misc_feature    870..877
                     /label=NotI
     misc_feature    877..882
                     /label=PstI
     primer_bind     940..959
                     /label=Verification reverse (VR) primer binding site
     misc_feature    1041..1723
                     /label=ColE1 origin
     misc_feature    1044..1663
                     /label=pBR322 origin
     CDS             1818..2678
                     /label=beta-lactamase ampicillin resistance marker
     promoter        2720..2748
                     /label=beta-lactamase (ampR, bla) promoter
     primer_bind     2801..2820
                     /label=Verification forward (VF2) primer binding site
     misc_feature    2919..2940
                     /label=BioBrick prefix
     misc_feature    2933..2933
                     /label=Ambiguity; should be a T
     misc_feature    2941..3044
                     /label=BBa_K257000
     misc_feature    2976..2981
                     /label=-35
     misc_feature    2997..3002
                     /label=-10
     misc_feature    3011..3011
                     /label=+1
     misc_feature    3011..3030
                     /label=important for activity
ORIGIN
        1 aaagaggaga aatactagat ggcttcctcc gaagacgtta tcaaagagtt catgcgtttc
       61 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      121 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      181 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      241 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      301 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      361 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      421 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      481 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
      541 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
      601 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
      661 gaacgtgctg aaggtcgtca ctccaccggt gcttaataac gctgatagtg ctagtgtaga
      721 tcgctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc
      781 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg
      841 gtgggccttt ctgcgtttat atactagaag cggccgctgc aggcttcctc gctcactgac
      901 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
      961 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     1021 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1081 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1141 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1201 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1261 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1321 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1381 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     1441 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     1501 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     1561 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     1621 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     1681 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     1741 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     1801 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     1861 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     1921 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     1981 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     2041 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     2101 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     2161 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     2221 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     2281 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     2341 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     2401 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     2461 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     2521 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     2581 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     2641 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     2701 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     2761 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     2821 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca gaatttcaga
     2881 taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc gcatctagag
     2941 tacgcggtac tggataaaca tttcaccact gtaaggaaaa taattcttat ttcgattgtc
     3001 ctttttaccc ttctcgttcg actcatagct gaacacaaca aaaa
//