LOCUS BBa_K258008 4900 bp DNA linear 08-May-2024 DEFINITION Export of recombinant proteins in Escherichia coli using ABC transporter of Erwinia chrysanthemi ACCESSION BBa_K258008 VERSION BBa_K258008.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 CDS 81..1805 /label=BBa_K258100 stem_loop 1825..1868 /label=stem_loop misc_feature 1814..1893 /label=BBa_B0010 terminator 1814..1893 /label=BBa_B0010 misc_feature 1906..1909 /label=conserved RBS 1902..1913 /label=BBa_B0034 CDS 1920..3263 /label=BBa_K258101 stem_loop 3283..3326 /label=stem_loop misc_feature 3272..3351 /label=BBa_B0010 terminator 3272..3351 /label=BBa_B0010 misc_feature 3364..3367 /label=conserved RBS 3360..3371 /label=BBa_B0034 CDS 3378..4763 /label=BBa_K258102 stem_loop 4783..4826 /label=stem_loop misc_feature 4772..4851 /label=BBa_B0010 terminator 4772..4851 /label=BBa_B0010 stem_loop 4867..4886 /label=T7 TE misc_feature 4893..4893 /label=stop polyA_site 4887..4900 /label=polya misc_feature 4860..4900 /label=BBa_B0012 terminator 4860..4900 /label=BBa_B0012 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaacgctt cctctgaacg cgatcgtagc ctgttcggcg 121 tgctgcgcca gttccgccgc tccttttggt ctgttggtat cttctctgcg gtgatcaacg 181 ttctgatgct ggcaccgtct gtttatatgc tgcaggtata tgaccgtgtt ctggcttctg 241 gtaatggcat caccctgctg atgctgaccc tgctgatggc aggtctgtgc gccttcatgg 301 gtgcactgga atgggttcgc tccctgctgg tcgttcgcct gggtactcgt atcgatctgg 361 cactgaacca ggacgtattc aatgcggcct ttgcgcgtaa cctggaagcg ggtgacggtc 421 gtgcgggtct ggccctgacc gatctgaccc tgctgcgtca gttcatcacg ggtaacgcgc 481 tgttcgcttt cttcgatgtt ccgtggttcc cgctgttcct gctggtgctg ttcctgctgc 541 acccatggct gggtatgctg gccctgggcg gtactgtggt gccgggtggt gttggcctgg 601 cggaaccggc gtccgaccag tctactgctg gtggcagcaa ccagcagtct cagcaggcca 661 ctcatctggc cgacgctcag ctgcgtaacg cggatgttat tgaagctatg ggcatgctgg 721 gcaacctgcg tcgtcgttgg ctggcacgcc actatcgttt catttccctg caaaacctgg 781 caagcgaacg tgctgcagcg gtcggcggtg cgagcaaata ttctcgcatt gctctgcaaa 841 gcctgatgct gggcctgggt gcactgctgg cgatcgacgg taaaatcacc cctggcatga 901 tgattgctgg cagcatcctg gttggtcgtg tgctgagccc gatcgatcag ctgatcggtg 961 tatggaaaca gtggtcttct gcccgtatcg catggcagcg cctgacgcgc ctgattgctg 1021 cgtatccgcc gcgtccggcg gctatggcac tgcctgcacc ggaaggccac ctgagcgtag 1081 aacaggtttc tctgcgtacc gctcagggca acacccgcct gcaaaacatc cacttctctc 1141 tgcaggctgg tgaaaccctg gtgatcctgg gcgcatctgg ttctggcaaa tcctccctgg 1201 ctcgtctgct ggttggcgcg cagtctccga cccagggtaa agtacgtctg gacggcgcag 1261 atctgaatca ggttgacaaa aacactttcg gcccgacgat cggctatctg ccgcaggatg 1321 ttcagctgtt taaaggctcc ctggcagaga acattgcgcg tttcggtgac gccgacccgg 1381 aaaaagttgt tgctgcggcc aaactggcgg gcgtgcacga actgatcctg tctctgccga 1441 acggttacga taccgaactg ggcgacggtg gtggtggcct gagcggcggt caacgccagc 1501 gtatcggtct ggctcgtgcc atgtacggcg atccgtgcct gctgatcctg gacgaaccga 1561 acgcttccct ggatagcgaa ggcgaccagg ctctgatgca agcaattgtt gcactgcaaa 1621 aacgtggcgc gaccgtagtt ctgatcaccc accgccctgc actgactacc ctggcccaga 1681 aaattctgat cctgcacgaa ggtcagcagc agcgtatggg tctggcgcgc gacgtgctga 1741 cggaactgca acagcgttcc gctgctaacc aggcacgtat gaacccgact gcggccatgc 1801 ctcagtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt 1861 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gaaagaggag aaatactaga 1921 tgactggcat ggacattacg acgcaggatg aactgaacga ggctgcgatg cgtgaccgtg 1981 cctcccgtga cgaagaacgt gcactgcgtc tgggttggtg gctggtactg gctggtttcg 2041 gtggtttcct gctgtgggcg ctgctggctc cactggacaa aggcgtggcg gtacagggca 2101 atgtcgtggt atctggcaat cgtaaagtta tccagcacat gcagggcggc atcgtggacc 2161 gcattcaagt taaggacggc gaccgcgtgg ctgcgggtca agttctgctg accctgaatg 2221 ctgtggacgc acgtaccact agcgaaggtc tgggttccca gtacgatcag ctgatcgcac 2281 gtgaagctcg tctgctggcg gagcagcgta accagagcag cctggcagcc acgcctcgtc 2341 tgactcaagc tcgccagcgt ccggaaatgg cagcgatcat tgcactgcaa gaggatctgc 2401 tgcgttcccg tcagcagagc ctgaaactgg aaattgacgg cgttcgcgcc agcatcgatg 2461 gcctggaaac cagcctgggc gctctgcaaa aggtaatgtc cagcaaacag tccgagcaag 2521 ccaccctgtc ccagcagctg caaggtctgc gtccgctggc tgctgacaac tacgttccgc 2581 gcaacaaaat gctggaaacc gaacgcctgt ttgcgcaagt atctggtgag ctggctcaga 2641 cttctggtga agtgggtcgt acccgtcgtg atattcagca gcagaaactg cgtattgctc 2701 agcgtcagca agaatacgac aaagaagtaa actccgaact gtctgacgtt caggccaaac 2761 tgaacgaagt gatcagccag cgtgaaaaag ccgatttcaa cctggctaac gttcaggtgc 2821 gcgctccggt cgcgggcacc gtggtagaca tgaaaatctt taccgagggc ggtgtaatcg 2881 cgccaggtca ggtaatgatg gatattgttc cggaggatca gccgctgctg gtagacggtc 2941 gcattcctgt agaaatggtt gataaagttt ggagcggtct gccagtagag ctgcaattca 3001 ctgcgttctc tcagtctacg accccgcgtg ttccgggcac cgtcaccctg ctgtctgcag 3061 accgtctggt ggatgagaag gatggtaccc cgtactacgg tctgcgcatc caggtttccg 3121 aggagggtaa acgcagcctg cacggcctgg aaattaaacc tggcatgcct gttcagggct 3181 tcgtacgtac cggtgaacgt tctttcatta actatctgtt caaaccgctg atggaccgta 3241 tgcacctggc tctgaccgag gaatactaga gccaggcatc aaataaaacg aaaggctcag 3301 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagaga 3361 aagaggagaa atactagatg cgtcgtaaag cggttctgct gactgtagca ctgtttctgc 3421 caggtgtcgg tgcgcaggcg atgggcctgc tggacgcctg ggaactggcg ctgcgtaatg 3481 atgctcaact gcgtgctgcg ggtttcgagc gcgacgcggg tcaggaggaa gttgctatcg 3541 gtcgcgcagg cctgctgccg tctctgcaat actcttacgg cgccaactac tctcactcta 3601 aagttacgca gcgcgaccgt accaccgata acactaccca gcgtgactac aacaactatg 3661 tgtctaccct gactctgcgc cagccgctgc tggactacgc tgcttgggcc cgttatcaac 3721 agggcgtaac ccgtaaactg atggccgacc agcgctttcg tgaacgctcc caggacctga 3781 tggtccgcct gtaccaggca tggagcgacg cgctgctggc acaggagaaa ctgatgctgc 3841 tggacgcaca gcgccgtgct taccaagaac agctggctct gaaccgtcgt ctgctggctg 3901 ccggtgaagg tacgcagacc gatctgcgtg aaactgaggc gcgctatacc gttatcgaag 3961 ctcagcgtat cgaacaggaa gacacgctgg atgcagctat gactgctctg gagaatatga 4021 tcggttctcc tctgcaaatt caagacctga gcccgctggt actggaagaa ctgcctgata 4081 atgtaaccga aaaccgccct ctgacccaat ggcgtgagct ggccgtacgt cacaacgcga 4141 aactggctgt ccagcgtgag aatgttgact attcccgcta tgaaattgag cgcaaccgcg 4201 ctggtcacct gccgtctgtt gacctggtgg cgtctacccg caactccctg tctgaaagcg 4261 agtacaacta caaccagaaa tatgataccc agacggtagg tgttcaggtt cgtgtaccgc 4321 tgtatagcgg cggtgctgtt agcgctagca tgcgtcaggc caccgcagaa tacgcccaaa 4381 accaggcgga actggataac caaacgcgcc agaccttcgc cgaactgcgc cgtcagttca 4441 acctgtgtat caacggtagc gcgaaaatcc gcgcctggca gatgagcgta actacggctg 4501 ctgaagcgat ccgtgcgacc cgccagagcg ttgcgggcgg tgaacgcatc aacctggacg 4561 tactgatggc ggagcaggag tggtataacg cccgtcgcga actgactgaa gtgaagtatc 4621 gttggctgca agcttggctg aacctgcgtt acactgccgg tactctgaac gaccaggaca 4681 tgatgcagct ggctgcgtgg ttccagccgg cggacgccgc taacaaaaac cgtgctaaaa 4741 ccgtatctgg caataaagtg aactactaga gccaggcatc aaataaaacg aaaggctcag 4801 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 4861 cacactggct caccttcggg tgggcctttc tgcgtttata //