BBa_J61100
1
BBa_J61100
Ribosome Binding Site Family Member
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
N/A
{{JCA_Arkin_RBSFamily}}
false
true
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_R0081
1
AraC O2
Inhibitor (AraC loop attachment with O2 site)
2004-01-29T12:00:00Z
2015-05-08T01:14:15Z
Released HQ 2013
this piece of DNA contains a "dead" part of coding region for AraC, and a functional Operator2 site. when attached to the rest of the promoter region, this part allows for the binding and loop formation that would result in repression of the araC promoter
false
false
_1_
0
24
7
In stock
false
1) changed ATG start codon to TAG stop
2) 6 random deletions upstream of putative araC binding sites to preserve spacing for loop back (BB ends will add 6 base pairs); spacing crucial in matching up O2 and I2 binding sites (turns of DNA helix)
3) designed to attach to R0080 to enable all (positive and negative) regulatory functions, and to form "loop back"
true
Sara Neves (Fighting Darwins)
annotation331786
1
stem_loop
range331786
1
22
37
annotation331787
1
stem_loop
range331787
1
57
72
annotation331785
1
defunct araC
range331785
1
1
78
BBa_K259012
1
BBa_K259012
AraC-RBS-OsmE
2009-10-14T11:00:00Z
2015-05-08T01:11:42Z
AraC - occurs naturally in E.coli genome. This part was created by assembling parts R0081 and R0080 together.
RBS - Designed from the Anderson family. A strong RBS for high translation rates.
OsmE - Obtained via PCR gene cloning from the E.coli MG1655 genome.
This part will express the protein OsmE that is found on the Outer Membrane of E.coli cells. This protein is osmotically inducible but of unknown function.
The part currently carries an illegal EcoRI site that can be mutated out (contact us for information).
It is useful for protein fusions when the user wants to deliver proteins to the outer membrane. For protein fusions the part can be used with Bioscaffolds designed by the BCCS-Bristol iGEM'09 team, that facilitate protein fusion (<partinfo>Bba_K259002</partinfo><partinfo>Bba_K259004</partinfo>).
At the time of writing only part Bba_K259002 is supported.
false
false
_357_
0
5318
9
It's complicated
false
Part was designed using 2-way ligation to avoid EcoRI site.
false
Petros Mina
component2041255
1
BBa_K259011
component2041254
1
BBa_J61100
component2041253
1
BBa_R0080
component2041246
1
BBa_R0081
annotation2041254
1
BBa_J61100
range2041254
1
349
360
annotation2041253
1
BBa_R0080
range2041253
1
192
340
annotation2041255
1
BBa_K259011
range2041255
1
367
708
annotation2041246
1
BBa_R0081
range2041246
1
1
183
BBa_K259011
1
BBa_K259011
OsmE-Outer Membrane Protein in E.coli
2009-10-14T11:00:00Z
2015-05-08T01:11:42Z
This part occurs naturally in the E.coli genome at 39.27 minutes in the counterclockwise direction. [http://ecogene.org/geneinfo.php?eg_id=EG10044 | EG10044 EcoGENE accession number]
This is an Outer Membrane monomeric protein in E.coli. It is an osmotically inducible lipoprotein, not toxic but it's usage is unknown.
false
false
_357_
0
5318
9
It's complicated
false
This part carries an unmutated EcoRI site.
false
Petros Mina
BBa_R0080
1
AraC
Promoter (AraC regulated)
2004-01-27T12:00:00Z
2015-05-08T01:14:15Z
GenBank: J01641 (www.ncbi.nlm.nih.gov)
Released HQ 2013
AraC operator, truncated to include araO1, araI1, araI2, c-amp1, and c-amp2 sites. This operator should *activate* transcription in the presence of AraC; b/c the operator lacks the araO2 site, there should not be araC-mediated repression.
false
false
_1_
0
24
7
In stock
false
true
Sara Neves (Fighting Darwins)
annotation301457
1
araO1
range301457
1
6
44
annotation301462
1
ara1 and ara2
range301462
1
73
101
annotation301458
1
c-amp1
range301458
1
43
72
annotation308602
1
-10
range308602
1
136
141
annotation301456
1
c-amp2
range301456
1
4
29
annotation308601
1
-35
range308601
1
113
118
BBa_J61100_sequence
1
aaagaggggaca
BBa_K259011_sequence
1
atgaacaagaatatggcaggaattctgagtgcagcggcggtattaaccatgctggcgggttgtacggcttatgatcgtaccaaagaccagtttgtacagcctgtggtgaaagacgtcaaaaaaggcatgagccgggcgcaggttgcacaaattgcgggtaaaccttcgtctgaagtgagcatgatccatgctcgcggtacttgccagacctacatcctgggtcaacgtgatggtaaagcagaaacctactttgtcgcgttagatgataccggacatgtcatcaactccggttatcagacctgtgctgaatacgacactgatccacaggctgcgaagtaataa
BBa_K259012_sequence
1
caggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgctacagacattgccgtcactggtctttactggctcttctcgctaaccaaaccggtaaccgcttattaaagcattctgtaacaaagcgggaccaaagccatgacaaaactactagaggcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccattactagagaaagaggggacatactagatgaacaagaatatggcaggaattctgagtgcagcggcggtattaaccatgctggcgggttgtacggcttatgatcgtaccaaagaccagtttgtacagcctgtggtgaaagacgtcaaaaaaggcatgagccgggcgcaggttgcacaaattgcgggtaaaccttcgtctgaagtgagcatgatccatgctcgcggtacttgccagacctacatcctgggtcaacgtgatggtaaagcagaaacctactttgtcgcgttagatgataccggacatgtcatcaactccggttatcagacctgtgctgaatacgacactgatccacaggctgcgaagtaataa
BBa_R0081_sequence
1
caggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgctacagacattgccgtcactggtctttactggctcttctcgctaaccaaaccggtaaccgcttattaaagcattctgtaacaaagcgggaccaaagccatgacaaaac
BBa_R0080_sequence
1
gcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccat
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z