LOCUS BBa_K260002 6701 bp DNA linear 01-Jun-2024 DEFINITION pTetFlp. [anhydrotetracycline] -> FLP(F70L) ACCESSION BBa_K260002 VERSION BBa_K260002.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=suffix misc_feature 75..436 /label=pSC101 origin CDS 820..1770 /label=repA CDS 1812..2435 /label=TetR CDS 2445..3470 /label=HygR CDS 4144..4938 /label=KanR CDS 5245..6516 /label=FLP(F70L) stem_loop 5193..5224 /label=T4_32 terminator promoter 6517..6643 /label=tet-promoter misc_feature 6680..6701 /label=prefix ORIGIN 1 tactagtagc ggccgctgca gaaaaaaaaa ccccgcccct gacagggcgg ggtttttttt 61 ctcgagtcta gacagtaaga cgggtaagcc tgttgatgat accgctgcct tactgggtgc 121 attagccagt ctgaatgacc tgtcacggga taatccgaag tggtcagact ggaaaatcag 181 agggcaggaa ctgctgaaca gcaaaaagtc agatagcacc acatagcaga cccgccataa 241 aacgccctga gaagcccgtg acgggctttt cttgtattat gggtagtttc cttgcatgaa 301 tccataaaag gcgcctgtag tgccatttac ccccattcac tgccagagcc gtgagcgcag 361 cgaactgaat gtcacgaaaa agacagcgac tcaggtgcct gatggtcgga gacaaaagga 421 atattcagcg atttgcccga gcttgcgagg gtgctactta agcctttagg gttttaaggt 481 ctgttttgta gaggagcaaa cagcgtttgc gacatccttt tgtaatactg cggaactgac 541 taaagtagtg agttatacac agggctggga tctattcttt ttatcttttt ttattctttc 601 tttattctat aaattataac cacttgaata taaacaaaaa aaacacacaa aggtctagcg 661 gaatttacag agggtctagc agaatttaca agttttccag caaaggtcta gcagaattta 721 cagataccca caactcaaag gaaaaggact agtaattatc attgactagc ccatctcaat 781 tggtatagtg attaaaatca cctagaccaa ttgagatgta tgtctgaatt agttgttttc 841 aaagcaaatg aactagcgat tagtcgctat gacttaacgg agcatgaaac caagctaatt 901 ttatgctgtg tggcactact caaccccacg attgaaaacc ctacaaggaa agaacggacg 961 gtatcgttca cttataacca atacgctcag atgatgaaca tcagtaggga aaatgcttat 1021 ggtgtattag ctaaagcaac cagagagctg atgacgagaa ctgtggaaat caggaatcct 1081 ttggttaaag gctttgagat tttccagtgg acaaactatg ccaagttctc aagcgaaaaa 1141 ttagaattag tttttagtga agagatattg ccttatcttt tccagttaaa aaaattcata 1201 aaatataatc tggaacatgt taagtctttt gaaaacaaat actctatgag gatttatgag 1261 tggttattaa aagaactaac acaaaagaaa actcacaagg caaatataga gattagcctt 1321 gatgaattta agttcatgtt aatgcttgaa aataactacc atgagtttaa aaggcttaac 1381 caatgggttt tgaaaccaat aagtaaagat ttaaacactt acagcaatat gaaattggtg 1441 gttgataagc gaggccgccc gactgatacg ttgattttcc aagttgaact agatagacaa 1501 atggatctcg taaccgaact tgagaacaac cagataaaaa tgaatggtga caaaatacca 1561 acaaccatta catcagattc ctacctacgt aacggactaa gaaaaacact acacgatgct 1621 ttaactgcaa aaattcagct caccagtttt gaggcaaaat ttttgagtga catgcaaagt 1681 aagcatgatc tcaatggttc gttctcatgg ctcacgcaaa aacaacgaac cacactagag 1741 aacatactgg ctaaatacgg aaggatctga gctagctgaa gttaccatca cggaaaaagg 1801 ttatgctgct tttaagaccc actttcacat ttaagttgtt tttctaatcc gcatatgatc 1861 aattcaaggc cgaataagaa ggctggctct gcaccttggt gatcaaataa ttcgatagct 1921 tgtcgtaata atggcggcat actatcagta gtaggtgttt ccctttcttc tttagcgact 1981 tgatgctctt gatcttccaa tacgcaacct aaagtaaaat gccccactgc gctgagtgca 2041 tataatgcat tctctagtga aaaaccttgt tggcataaaa aggctaattg attttcgaga 2101 gtttcatact gtttttctgt aggccgtgta cctaaatgta cttttgctcc atcgcgatga 2161 cttagtaaag cacatctaaa acttttagcg ttattacgta aaaaatcttg ccagctttcc 2221 ccttctaaag ggcaaaagtg agtatggtgc ctatctaaca tctcaatggc taaggcgtcg 2281 agcaaagccc gcttattttt tacatgccaa tacaatgtag gctgctctac acctagcttc 2341 tgggcgagtt tacgggttgt taaaccttcg attccgacct cattaagcag ctctaatgcg 2401 ctgttaatca ctttactttt atctaaacga gacatcatta attcctattc ctttgccctc 2461 ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca gccatcggtc 2521 cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc tccggatcgg 2581 acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag 2641 ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg cggcgatcct 2701 gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca agccaaccac 2761 ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat cgcctcgctc 2821 cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga gccgaaatcc 2881 gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag ctcatcgaga 2941 gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg atacacatgg 3001 ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat tccttgcggt 3061 ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc atccatggcc 3121 tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg caacgtgaca 3181 ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc aatgtcaagc 3241 acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg atctttgtag 3301 aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac atcgaagctg 3361 aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct gtcgaacttt 3421 tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat actcttcctt 3481 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa 3541 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct 3601 gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca 3661 ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag 3721 atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc 3781 aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc 3841 taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc 3901 ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa 3961 gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc 4021 acacccgccg cgcttaatgc gccgctacag ggcgcgtgga gatccgtgac gcagtagcgg 4081 taaacggcag acaaaaaaaa tgtcgcacaa tgtgcgccat ttttcacttc acaggtcaag 4141 ctttcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc 4201 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc 4261 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat 4321 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt 4381 cacgacgaga tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg 4441 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg 4501 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc 4561 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag 4621 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc 4681 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag 4741 ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag 4801 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg 4861 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa 4921 tccatcttgt tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc 4981 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc 5041 taccttacca gagggcgccc cagctggcaa ttccggttcg ctgcgatatc ttaggatctc 5101 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc attttattta 5161 tgcttgtaaa ccgtttttgt gaaaaaattt ttaaaataaa aaaggggacc tctagggtcc 5221 ccaattaatt agtaatataa tctattatat gcgtctattt atgtaggatg aaaggtagtc 5281 tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc cttcagcact 5341 accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat ccttcaatgc 5401 tatcatttcc tttgatattg gatcatatgc atagtaccga gaaactagtg cgaagtagtg 5461 atcaggtatt gctgttatct gatgagtata cgttgtcctg gccacggcag aagcacgctt 5521 atcgctccaa tttcccacaa cattagtcaa ctccgttagg cccttcattg aaagaaatga 5581 ggtcatcaaa tgtcttccaa tgtgagattt tgggccattt tttatagcaa agattgaata 5641 aggcgcattt ttcttcaaag ctttgttgta cgatctgact aagttatctt ttaataattg 5701 gtattcctgc ttgttgcttg aagaattgcc ggtcctattt actcgtttta ggactggttc 5761 agaattcctc aaaaattcat ccaaatatac aagtggatcg atcctacccc ttgcgctaaa 5821 gaagtatatg tgcctactaa cgcttgtctt tgtctctgtc actaaacact ggattattac 5881 tcccagatac ttattttgga ctaatttaaa tgatttcgga tcaacgttct taatatcgct 5941 gaatcttcca caattgatga aagtagctag gaagaggaat tggtataaag tttttgtttt 6001 tgtaaatctc gaagtatact caaacgaatt tagtattttc tcagtgatct cccagatgct 6061 ttcaccctca cttagaagtg ctttaagcat ttttttactg tggctatttc ccttatctgc 6121 ttcttccgat gattcgaact gtaattgcaa actacttaca atatcagtga tatcagattg 6181 atgtttttgt ccatagtaag gaataattgt aaattcccaa gcaggaatca atttctttaa 6241 tgaggcttcc agaattgttg ctttttgcgt cttgtattta aactgcagtg acttgttgac 6301 aatatccaaa ctcagcgaat tgcttatgat agtattatag ctcatgaatg tggctctctt 6361 gattgctgtt ccgttatgtg taatcatcca acataaatag gttagttcag cagcacataa 6421 tgctattttc tcacctgaag gtctttcaaa cctttccaca aactgacgaa caagcacctt 6481 aggtggtgtt ttacataata tatcaaattg tggcattttt tgccctcgtt atctagattt 6541 ttgtcgaact attcatttca cttttctcta tcactgatag ggagtggtaa aataactcta 6601 tcaatgatag agtgtcaaca aaaattagga attaatcatc tggccattcg atggtgtcgg 6661 gtgatatcaa ttcctcgagg aattcgcggc cgcttctaga g //