LOCUS BBa_K260003 6345 bp DNA linear 14-Jun-2024 DEFINITION pRhaFlp. [L-rhamnose] -> FLP(F70L) ACCESSION BBa_K260003 VERSION BBa_K260003.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=suffix CDS 108..1058 /label=repA misc_feature 1442..1803 /label=pSC101 origin CDS 1854..2648 /label=KanR stem_loop 2902..2933 /label=T4_32 terminator CDS 2954..4225 /label=FLP(F70L) CDS 4513..5349 /label=rhaS CDS 5333..6271 /label=rhaR stem_loop 6272..6303 /label=T4_32 terminator promoter 4226..4512 /label=rhamnose-promoter misc_feature 6324..6345 /label=prefix ORIGIN 1 tactagtagc ggccgctgca gaaaaaaaaa ccccgcccct gacagggcgg ggtttttttt 61 ctcgagaagc agcataacct ttttccgtga tggtaacttc agctagctca gatccttccg 121 tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa 181 ccattgagat catgcttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc 241 tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttacg taggtaggaa 301 tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt 361 tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg 421 cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt 481 ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc aagcattaac 541 atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt 601 agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca 661 tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca 721 ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc 781 tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt 841 gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg agcgtattgg 901 ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt 961 agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc 1021 gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga 1081 ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt 1141 tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct 1201 agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt 1261 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg 1321 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt 1381 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg 1441 ggcaaatcgc tgaatattcc ttttgtctcc gaccatcagg cacctgagtc gctgtctttt 1501 tcgtgacatt cagttcgctg cgctcacggc tctggcagtg aatgggggta aatggcacta 1561 caggcgcctt ttatggattc atgcaaggaa actacccata atacaagaaa agcccgtcac 1621 gggcttctca gggcgtttta tggcgggtct gctatgtggt gctatctgac tttttgctgt 1681 tcagcagttc ctgccctctg attttccagt ctgaccactt cggattatcc cgtgacaggt 1741 cattcagact ggctaatgca cccagtaagg cagcggtatc atcaacaggc ttacccgtct 1801 tactggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag 1861 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa 1921 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc 1981 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa 2041 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga 2101 tcctcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc 2161 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct 2221 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc 2281 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac 2341 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca 2401 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc 2461 tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc 2521 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag 2581 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt 2641 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcag atcttgatcc cctgcgccat 2701 cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc taccttacca 2761 gagggcgccc cagctggcaa ttccggttcg ctgcgatatc ttaggatctc ccatcggtga 2821 tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc attttattta tgcttgtaaa 2881 ccgttttgtg aaaaaatttt taaaataaaa aaggggacct ctagggtccc caattaatta 2941 gtaatataat ctattatatg cgtctattta tgtaggatga aaggtagtct agtacctcct 3001 gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct 3061 gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct 3121 ttgatattgg atcatatgca tagtaccgag aaactagtgc gaagtagtga tcaggtattg 3181 ctgttatctg atgagtatac gttgtcctgg ccacggcaga agcacgctta tcgctccaat 3241 ttcccacaac attagtcaac tccgttaggc ccttcattga aagaaatgag gtcatcaaat 3301 gtcttccaat gtgagatttt gggccatttt ttatagcaaa gattgaataa ggcgcatttt 3361 tcttcaaagc tttgttgtac gatctgacta agttatcttt taataattgg tattcctgct 3421 tgttgcttga agaattgccg gtcctattta ctcgttttag gactggttca gaattcctca 3481 aaaattcatc caaatataca agtggatcga tcctacccct tgcgctaaag aagtatatgt 3541 gcctactaac gcttgtcttt gtctctgtca ctaaacactg gattattact cccagatact 3601 tattttggac taatttaaat gatttcggat caacgttctt aatatcgctg aatcttccac 3661 aattgatgaa agtagctagg aagaggaatt ggtataaagt ttttgttttt gtaaatctcg 3721 aagtatactc aaacgaattt agtattttct cagtgatctc ccagatgctt tcaccctcac 3781 ttagaagtgc tttaagcatt tttttactgt ggctatttcc cttatctgct tcttccgatg 3841 attcgaactg taattgcaaa ctacttacaa tatcagtgat atcagattga tgtttttgtc 3901 catagtaagg aataattgta aattcccaag caggaatcaa tttctttaat gaggcttcca 3961 gaattgttgc tttttgcgtc ttgtatttaa actgcagtga cttgttgaca atatccaaac 4021 tcagcgaatt gcttatgata gtattatagc tcatgaatgt ggctctcttg attgctgttc 4081 cgttatgtgt aatcatccaa cataaatagg ttagttcagc agcacataat gctattttct 4141 cacctgaagg tctttcaaac ctttccacaa actgacgaac aagcacctta ggtggtgttt 4201 tacataatat atcaaattgt ggcataatgt gatcctgctg aatttcatta cgaccagtct 4261 aaaaagcgcc tgaattcgcg accttctcgt tactgacagg aaaatgggcc attggcaacc 4321 agggaaagat gaacgtgatg atgttcacaa tttgctgaat tgtggtgatg tgatgctcac 4381 cgcatttcct gaaaattcac gctgtatctt gaaaaatcga cgttttttac gtggttttcc 4441 gtcgaaaatt taaggtaaga acctgacctc gtgattacta tttcgccgtg ttgacgacat 4501 caggaggcca gtatgaccgt attacatagt gtggattttt ttccgtctgg taacgcgtcc 4561 gtggcgatag aaccccggct cccgcaggcg gattttcctg aacatcatca tgattttcat 4621 gaaattgtga ttgtcgaaca tggcacgggt attcatgtgt ttaatgggca gccctatacc 4681 atcaccggtg gcacggtctg tttcgtacgc gatcatgatc ggcatctgta tgaacatacc 4741 gataatctgt gtctgaccaa tgtgctgtat cgctcgccgg atcgatttca gtttctcgcc 4801 gggctgaatc agttgctgcc acaagagctg gatgggcagt atccgtctca ctggcgcgtt 4861 aaccacagcg tattgcagca ggtgcgacag ctggttgcac agatggaaca gcaggaaggg 4921 gaaaatgatt taccctcgac cgccagtcgc gagatcttgt ttatgcaatt actgctcttg 4981 ctgcgtaaaa gcagtttgca ggagaacctg gaaaacagcg catcacgtct caacttgctt 5041 ctggcctggc tggaggacca ttttgccgat gaggtgaatt gggatgccgt ggcggatcaa 5101 ttttctcttt cactgcgtac gctacatcgg cagcttaagc agcaaacggg actgacgcct 5161 cagcgatacc tgaaccgcct gcgactgatg aaagcccgac atctgctacg ccacagcgag 5221 gccagcgtta ctgacatcgc ctatcgctgt ggattcagcg acagtaacca cttttcgacg 5281 ctttttcgcc gagagtttaa ctggtcaccg cgtgatattc gccagggacg ggatggcttt 5341 ctgcaataac gcgaatcttc tcaacgtatt tgtacgccat attgcgaata atcaacttcg 5401 ttctctggcc gaggtagcca cggtggcgca tcagttaaaa cttctcaaag atgatttttt 5461 tgccagcgac cagcaggcag tcgctgtggc tgaccgttat ccgcaagatg tctttgctga 5521 acatacacat gatttttgtg agctggtgat tgtctggcgc ggtaatggcc tgcatgtact 5581 caacgatcgc ccttatcgca ttacccgtgg cgatctcttt tacattcatg ctgacgataa 5641 acactcctac gcttccgtta acgatctggt tttgcagaat attatttatt gcccggagcg 5701 tctgaagctg aatcttgact ggcagggggc gattccggga tttaacgcca gcgcagggca 5761 accacactgg cgcttaggta gcatggggat ggcgcaggcg cggcaggtta tcggtcagct 5821 tgagcatgaa agtagtcagc atgtgccgtt tgctaacgaa atggctgagt tgctgttcgg 5881 gcagttggtg atgttgctga atcgccatcg ttacaccagt gattcgttgc cgccaacatc 5941 cagcgaaacg ttgctggata agctgattac ccggctggcg gctagcctga aaagtccctt 6001 tgcgctggat aaattttgtg atgaggcatc gtgcagtgag cgcgttttgc gtcagcaatt 6061 tcgccagcag actggaatga ccatcaatca atatctgcga caggtcagag tgtgtcatgc 6121 gcaatatctt ctccagcata gccgcctgtt aatcagtgat atttcgaccg aatgtggctt 6181 tgaagatagt aactattttt cggtggtgtt tacccgggaa accgggatga cgcccagcca 6241 gtggcgtcat ctcaattcgc agaaagatta attggggacc ctagaggtcc ccttttttat 6301 tttgatatca cccgacactc gaggaattcg cggccgcttc tagag //