LOCUS       BBa_K260008             2548 bp    DNA     linear       21-May-2024
DEFINITION  pMA-RQ-@CC2FosMB-10000bp
ACCESSION   BBa_K260008
VERSION     BBa_K260008.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=suffix
     misc_feature    22..91
                     /label=BBa_K260001 (pCC2FosMB) 3' homology FRT-10000bp
     misc_feature    92..97
                     /label=PvuII site
     misc_feature    2451..2456
                     /label=PvuII site
     CDS             1087..1947
                     /label=AmpR
     misc_feature    272..939
                     /label=ColE1 origin
     misc_feature    2457..2526
                     /label=BBa_K260001 (pCC2FosMB) 5' homology FRT-10000bp
     misc_feature    2527..2548
                     /label=prefix
ORIGIN
        1 tactagtagc ggccgctgca gtttaataat ataaaatcat ttattgtaat taaccaaatt
       61 aatgttaaag aagaaaaatt acatgatcag ccagctggag ctcctgggcc tcatgggcct
      121 tccgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taacatggtc
      181 atagctgttt ccttgcgtat tgggcgctct ccgcttcctc gctcactgac tcgctgcgct
      241 cggtcgttcg ggtaaagcct ggggtgccta atgagcaaaa ggccagcaaa aggccaggaa
      301 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
      361 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
      421 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
      481 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
      541 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
      601 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
      661 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
      721 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
      781 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
      841 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
      901 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
      961 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     1021 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     1081 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     1141 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     1201 tggccccagt gctgcaatga taccgcgaga accacgctca ccggctccag atttatcagc
     1261 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     1321 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     1381 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     1441 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     1501 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     1561 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     1621 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     1681 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     1741 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     1801 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     1861 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     1921 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     1981 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     2041 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     2101 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     2161 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgg ccgctacagg
     2221 gcgctcccat tcgccattca ggctgcgcaa ctgttgggaa gggcgtttcg gtgcgggcct
     2281 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
     2341 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gacgtaatac
     2401 gactcactat agggcgaatt ggcggaaggc cgtcaaggcc gcatggtacc cagctggaga
     2461 atcgtttgaa cccaggaggt ggaggttgca gtgagccgag attgtgccac tgcactccag
     2521 cctggggaat tcgcggccgc ttctagag
//