LOCUS       BBa_K260015              334 bp    DNA     linear       22-May-2024
DEFINITION  P_FRT_dhfr.   (strong promoter, translated FRT site, trimethoprim 
            resistance)
ACCESSION   BBa_K260015
VERSION     BBa_K260015.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100 (strong promoter)
     RBS             44..55
                     /label=BBa_B0034 (Elowitz RBS)
     misc_feature    62..64
                     /label=ATG_Start
     misc_feature    66..99
                     /label=translated FRT site
     CDS             101..334
                     /label=optimised TmpR
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatgggaagt tcctattctc tagaaagtat aggaacttct ggtcagtctt ccgacgaagc
      121 gaacgctccg gttgctggcc agtttgcgct gccgctgtct gcaacctttg gcctgggcga
      181 tcgcgtacgc aagaaatctg gtgccgcttg gcagggtcaa gttgtgggtt ggtattgcac
      241 caaactgact ccagaaggct atgcggttga gtccgaatcc cacccaggct ctgtgcaaat
      301 ttatccagtg gctgcactgg aacgtgtggc ctaa
//