BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K266005
1
BBa_K266005
PAI+LasR -> LasI & AI+LuxR --| LasI
2009-10-12T11:00:00Z
2015-05-08T01:11:43Z
The biobricks BBa_K091146 and BBa_S03154 where used to build this biobrick
BBa_K091146 promoter (PAI+LasR inducible & AI+LuxR repressible) controls the expresion of LasI enzyme, no terminator
false
false
_363_
0
1955
9
It's complicated
true
None
false
UNAM FCIENCIAS-CINVESTAV IRA
component2246677
1
BBa_K091146
component2246684
1
BBa_S03154
annotation2246677
1
BBa_K091146
range2246677
1
1
126
annotation2246684
1
BBa_S03154
range2246684
1
135
819
BBa_K091146
1
BBa_K091146
pLas/Lux Hybrid Promoter
2008-07-16T11:00:00Z
2015-05-08T01:08:38Z
The LuxR binding site is from BBa_R0062 and the pLas' promoter is from the region upstream of the LasI gene in Pseudomonas aeruginosa.
Released HQ 2013
K091146 is a modified version of the pLas' promoter (BBa_K091117) containing the addition of a luxR binding site between the -35 and -10 regions. This promoter is expected to be activated in the presence of PAI-1 and LasR and repressed in the presence of AI-1 and LuxR.
false
false
_191_
0
3067
9
In stock
true
The Lux box was placed adjacent to the LasR binding site with the aim of minimizing cross-talk in the presence of both PAI-1 and AI-1; since the LasR site should have a higher affinity, we hope that the binding of PAI-1 will competitively exclude the binding of AI-1.
true
Erin Feeney
annotation1967358
1
Transcriptional Start
range1967358
1
125
125
annotation1967354
1
LasR
range1967354
1
75
93
annotation1967356
1
-10
range1967356
1
113
118
annotation1967357
1
-35
range1967357
1
86
91
annotation1967355
1
LuxR
range1967355
1
94
113
BBa_C0078
1
lasI
autoinducer synthetase for PAI from Pseudomonas aeruginosa
2004-01-27T12:00:00Z
2015-08-31T04:07:24Z
www.ncbi.nlm.nih.gov
Released HQ 2013
coding region for lasI protein, which produces the chemical signal AI-1
false
false
_1_
0
24
7
In stock
false
true
Chris Walsh (Fighting Darwins)
annotation305970
1
LasI
range305970
1
1
603
annotation2214003
1
Help:Barcodes
range2214003
1
643
667
annotation306608
1
LVA
range306608
1
604
636
BBa_S03154
1
BBa_S03154
B0034.C0078
2004-07-22T11:00:00Z
2015-05-08T01:14:20Z
Released HQ 2013
-- No description --
false
false
_11_1_
0
60
7
In stock
false
true
Jennifer Braff
component940972
1
BBa_C0078
component940962
1
BBa_B0034
annotation940962
1
BBa_B0034
range940962
1
1
12
annotation940972
1
BBa_C0078
range940972
1
19
660
BBa_K266005_sequence
1
tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcagtactagagaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
BBa_S03154_sequence
1
aaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
BBa_C0078_sequence
1
atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K091146_sequence
1
tgttctcgtgtgaagccattgctctgatcttttggacgtttcttcgagcctagcaagggtccgggttcaccgaaatctatctcatttgctagtacctgtaggatcgtacaggtataaattcttcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z