LOCUS       BBa_K272502             1615 bp    DNA     linear       17-May-2024
DEFINITION  Constitutive expression cassette for TetR repressor and mRFP 
            1-terminator
ACCESSION   BBa_K272502
VERSION     BBa_K272502.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             65..681
                     /label=tetR
     misc_feature    682..715
                     /label=SsrA
     misc_feature    722..746
                     /label=Help:Barcodes
     CDS             62..746
                     /label=BBa_C0040
     misc_feature    44..746
                     /label=BBa_S03879
     misc_feature    759..762
                     /label=conserved
     RBS             755..766
                     /label=BBa_B0034
     CDS             773..1447
                     /label=mrfp1
     misc_feature    1454..1478
                     /label=Help:Barcodes
     CDS             773..1453
                     /label=BBa_E1010
     stem_loop       1498..1541
                     /label=stem_loop
     misc_feature    1487..1566
                     /label=BBa_B0010
     terminator      1487..1566
                     /label=BBa_B0010
     stem_loop       1582..1601
                     /label=T7 TE
     misc_feature    1608..1608
                     /label=stop
     polyA_site      1602..1615
                     /label=polya
     misc_feature    1575..1615
                     /label=BBa_B0012
     terminator      1575..1615
                     /label=BBa_B0012
     misc_feature    755..1615
                     /label=BBa_I13507
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta
       61 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt
      121 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac
      181 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt
      241 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg
      301 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca
      361 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt
      421 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt
      481 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac
      541 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca
      601 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca
      661 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata
      721 acactgatag tgctagtgta gatcactact agagaaagag gagaaatact agatggcttc
      781 ctccgaagac gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa
      841 cggtcacgag ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac
      901 cgctaaactg aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc
      961 gcagttccag tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct
     1021 gaaactgtcc ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg
     1081 tgttgttacc gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa
     1141 actgcgtggt accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg
     1201 ggaagcttcc accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat
     1261 gcgtctgaaa ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc
     1321 taaaaaaccg gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc
     1381 ccacaacgaa gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac
     1441 cggtgcttaa taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata
     1501 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1561 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//