LOCUS       BBa_K273010             2881 bp    DNA     linear       19-May-2024
DEFINITION  RBS - kivd - RBS - adh2 - Ter
ACCESSION   BBa_K273010
VERSION     BBa_K273010.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=startcodon
     CDS             19..1662
                     /label=cds kivd_opt
     misc_feature    1663..1668
                     /label=stopcodon
     CDS             19..1668
                     /label=BBa_K273006
     misc_feature    1681..1684
                     /label=conserved
     RBS             1677..1688
                     /label=BBa_B0034
     misc_feature    1695..1697
                     /label=startcodon
     CDS             1695..2738
                     /label=cds
     misc_feature    2739..2744
                     /label=stopcodon
     CDS             1695..2744
                     /label=BBa_K273005
     stem_loop       2764..2807
                     /label=stem_loop
     misc_feature    2753..2832
                     /label=BBa_B0010
     terminator      2753..2832
                     /label=BBa_B0010
     stem_loop       2848..2867
                     /label=T7 TE
     misc_feature    2874..2874
                     /label=stop
     polyA_site      2868..2881
                     /label=polya
     misc_feature    2841..2881
                     /label=BBa_B0012
     terminator      2841..2881
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gtatactgtt ggtgattatc tgttggaccg tctgcatgag
       61 ctgggcattg aagaaatctt tggtgtgccg ggtgattaca atctgcaatt tctggatcag
      121 attatcagtc ataaagatat gaaatgggtg ggcaacgcga acgaactgaa cgcgagctac
      181 atggccgatg gttatgcgcg cactaagaaa gccgcggcct tcctgaccac ctttggtgtg
      241 ggtgaactga gcgccgtgaa tggcctggcg ggtagctacg ccgaaaatct gcccgtggtg
      301 gaaatcgtgg gcagcccgac tagcaaggtt cagaacgaag gcaagttcgt tcatcatacc
      361 ctggccgacg gcgatttcaa acactttatg aaaatgcacg aacccgttac tgcggcgcgt
      421 actctgttga ccgcggagaa cgccaccgtg gaaatcgatc gtgttctgag cgccctgttg
      481 aaagagcgca agcccgtgta tatcaatctg ccggttgatg ttgccgcggc caaagcggaa
      541 aaaccgagtc tgcccctgaa aaaggaaaat agtaccagta ataccagcga tcaggagatc
      601 ctgaacaaga tccaggaaag cctgaaaaat gccaagaaac cgatcgtgat tactggccat
      661 gagattatta gtttcggtct ggagaaaacc gtgacccaat tcattagcaa aaccaaactg
      721 cccattacca ccctgaactt cggtaaaagc agcgttgatg aggcgctgcc gagctttctg
      781 ggcatttaca atggtaccct gagcgaaccc aacctgaaag agtttgttga aagtgcggat
      841 ttcattctga tgctgggtgt taaactgacc gatagtagta ccggcgcctt cactcatcat
      901 ctgaatgaaa ataagatgat cagtctgaac attgatgaag gcaagatttt caacgaacgt
      961 atccaaaact ttgattttga gagtctgatc agtagtctgt tggacctgag cgagattgaa
     1021 tacaagggca agtacattga caagaagcaa gaagatttcg ttccgagtaa tgccctgttg
     1081 agtcaagatc gcctgtggca agcggtggaa aatctgaccc aaagcaacga aaccattgtg
     1141 gccgaacagg gcaccagctt tttcggtgcc agtagtattt ttctgaaaag taagagccac
     1201 ttcatcggtc aaccgctgtg gggcagcatt ggctatactt ttccggccgc cctgggcagc
     1261 caaatcgccg ataaagaaag ccgccatctg ttgtttattg gtgatggcag cctgcaactg
     1321 accgttcaag agctgggcct ggccattcgt gagaagatta atccgatttg tttcattatc
     1381 aacaacgatg gctatactgt tgaacgtgag attcatggtc cgaaccagag ttataatgac
     1441 attccgatgt ggaactacag caagctgccg gaaagcttcg gcgccaccga agatcgcgtg
     1501 gtgagtaaga ttgtgcgtac tgagaacgag ttcgttagtg tgatgaaaga ggcgcaagcc
     1561 gatccgaatc gtatgtattg gattgagctg attctggcga aagagggtgc gccgaaggtt
     1621 ctgaaaaaga tgggtaaact gtttgcggag cagaacaaaa gttaataata ctagagaaag
     1681 aggagaaata ctagatgtct attccagaaa ctcaaaaagc cattatcttc tacgaatcca
     1741 acggcaagtt ggagcataag gatatcccag ttccaaagcc aaagcccaac gaattgttaa
     1801 tcaacgtcaa gtactctggt gtctgccaca ccgatttgca cgcttggcat ggtgactggc
     1861 cattgccaac taagttacca ttagttggtg gtcacgaagg tgccggtgtc gttgtcggca
     1921 tgggtgaaaa cgttaagggc tggaagatcg gtgactacgc cggtatcaaa tggttgaacg
     1981 gttcttgtat ggcctgtgaa tactgtgaat tgggtaacga atccaactgt cctcacgctg
     2041 acttgtctgg ttacacccac gacggttctt tccaagaata cgctaccgct gacgctgttc
     2101 aagccgctca cattcctcaa ggtactgact tggctgaagt cgcgccaatc ttgtgtgctg
     2161 gtatcaccgt atacaaggct ttgaagtctg ccaacttgag agcaggccac tgggcggcca
     2221 tttctggtgc tgctggtggt ctaggttctt tggctgttca atatgctaag gcgatgggtt
     2281 acagagtctt aggtattgat ggtggtccag gaaaggaaga attgtttacc tcgctcggtg
     2341 gtgaagtatt catcgacttc accaaagaga aggacattgt tagcgcagtc gttaaggcta
     2401 ccaacggcgg tgcccacggt atcatcaatg tttccgtttc cgaagccgct atcgaagctt
     2461 ctaccagata ctgtagggcg aacggtactg ttgtcttggt tggtttgcca gccggtgcaa
     2521 agtgctcctc tgatgtcttc aaccacgttg tcaagtctat ctccattgtc ggctcttacg
     2581 tggggaacag agctgatacc agagaagcct tagatttctt tgccagaggt ctagtcaagt
     2641 ctccaataaa ggtagttggc ttatccagtt taccagaaat ttacgaaaag atggagaagg
     2701 gccaaattgc tggtagatac gttgttgaca cttctaaata ataatactag agccaggcat
     2761 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2821 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2881 a
//