BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K273006
1
kivd
α-ketoisovalerate decarboxylase (kivd) from <i>Lactococcus lactis</i> | codon optimized
2009-10-15T11:00:00Z
2015-05-08T01:11:44Z
Designed by the Uppsala iGEM Team 2009. Synthesized by Bioneer (www.bioneer.com)
α-ketoisovalerate decarboxylase (kivd) originally sequence from <i>Lactococcus lactis</i>. Codon optimized for use in <i>Synechocystis</i> and <i>E. coli</i>.
false
false
_373_
0
5250
9
It's complicated
true
The sequence was codon optimized using this [http://2009.igem.org/wiki/images/d/df/Codon_usage.txt codon-usage table]
false
Karl Brune
annotation2047067
1
cds kivd_opt
range2047067
1
1
1644
annotation2047066
1
startcodon
range2047066
1
1
3
annotation2047068
1
stopcodon
range2047068
1
1645
1650
BBa_K273010
1
BBa_K273010
RBS - kivd - RBS - adh2 - Ter
2009-10-15T11:00:00Z
2015-05-08T01:11:44Z
Assembly by the Uppsala iGEM Team 2009
RBS + α-ketoisovalerate decarboxylase (kivd) from Lactococcus lactis + RBS + Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from S. cerevisiae and Terminator
false
false
_373_
0
5250
9
It's complicated
false
-
false
Karl Brune
component2047169
1
BBa_K273005
component2047165
1
BBa_B0034
component2047172
1
BBa_B0012
component2047163
1
BBa_K273006
component2047170
1
BBa_B0010
component2047159
1
BBa_B0034
annotation2047163
1
BBa_K273006
range2047163
1
19
1668
annotation2047170
1
BBa_B0010
range2047170
1
2753
2832
annotation2047165
1
BBa_B0034
range2047165
1
1677
1688
annotation2047159
1
BBa_B0034
range2047159
1
1
12
annotation2047169
1
BBa_K273005
range2047169
1
1695
2744
annotation2047172
1
BBa_B0012
range2047172
1
2841
2881
BBa_K273005
1
adh2
Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from <i>S. cerevisiae</i>
2009-10-15T11:00:00Z
2015-05-08T01:11:44Z
<i>S. cerevisiae</i>
Alcohol Dehydrogenase 2 from <i>S. cerevisiae</i>
false
false
_373_
0
5250
9
It's complicated
true
-
false
Karl Brune
annotation2046992
1
stopcodon
range2046992
1
1045
1050
annotation2046975
1
startcodon
range2046975
1
1
3
annotation2046976
1
cds
range2046976
1
1
1044
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K273006_sequence
1
atgtatactgttggtgattatctgttggaccgtctgcatgagctgggcattgaagaaatctttggtgtgccgggtgattacaatctgcaatttctggatcagattatcagtcataaagatatgaaatgggtgggcaacgcgaacgaactgaacgcgagctacatggccgatggttatgcgcgcactaagaaagccgcggccttcctgaccacctttggtgtgggtgaactgagcgccgtgaatggcctggcgggtagctacgccgaaaatctgcccgtggtggaaatcgtgggcagcccgactagcaaggttcagaacgaaggcaagttcgttcatcataccctggccgacggcgatttcaaacactttatgaaaatgcacgaacccgttactgcggcgcgtactctgttgaccgcggagaacgccaccgtggaaatcgatcgtgttctgagcgccctgttgaaagagcgcaagcccgtgtatatcaatctgccggttgatgttgccgcggccaaagcggaaaaaccgagtctgcccctgaaaaaggaaaatagtaccagtaataccagcgatcaggagatcctgaacaagatccaggaaagcctgaaaaatgccaagaaaccgatcgtgattactggccatgagattattagtttcggtctggagaaaaccgtgacccaattcattagcaaaaccaaactgcccattaccaccctgaacttcggtaaaagcagcgttgatgaggcgctgccgagctttctgggcatttacaatggtaccctgagcgaacccaacctgaaagagtttgttgaaagtgcggatttcattctgatgctgggtgttaaactgaccgatagtagtaccggcgccttcactcatcatctgaatgaaaataagatgatcagtctgaacattgatgaaggcaagattttcaacgaacgtatccaaaactttgattttgagagtctgatcagtagtctgttggacctgagcgagattgaatacaagggcaagtacattgacaagaagcaagaagatttcgttccgagtaatgccctgttgagtcaagatcgcctgtggcaagcggtggaaaatctgacccaaagcaacgaaaccattgtggccgaacagggcaccagctttttcggtgccagtagtatttttctgaaaagtaagagccacttcatcggtcaaccgctgtggggcagcattggctatacttttccggccgccctgggcagccaaatcgccgataaagaaagccgccatctgttgtttattggtgatggcagcctgcaactgaccgttcaagagctgggcctggccattcgtgagaagattaatccgatttgtttcattatcaacaacgatggctatactgttgaacgtgagattcatggtccgaaccagagttataatgacattccgatgtggaactacagcaagctgccggaaagcttcggcgccaccgaagatcgcgtggtgagtaagattgtgcgtactgagaacgagttcgttagtgtgatgaaagaggcgcaagccgatccgaatcgtatgtattggattgagctgattctggcgaaagagggtgcgccgaaggttctgaaaaagatgggtaaactgtttgcggagcagaacaaaagttaataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K273010_sequence
1
aaagaggagaaatactagatgtatactgttggtgattatctgttggaccgtctgcatgagctgggcattgaagaaatctttggtgtgccgggtgattacaatctgcaatttctggatcagattatcagtcataaagatatgaaatgggtgggcaacgcgaacgaactgaacgcgagctacatggccgatggttatgcgcgcactaagaaagccgcggccttcctgaccacctttggtgtgggtgaactgagcgccgtgaatggcctggcgggtagctacgccgaaaatctgcccgtggtggaaatcgtgggcagcccgactagcaaggttcagaacgaaggcaagttcgttcatcataccctggccgacggcgatttcaaacactttatgaaaatgcacgaacccgttactgcggcgcgtactctgttgaccgcggagaacgccaccgtggaaatcgatcgtgttctgagcgccctgttgaaagagcgcaagcccgtgtatatcaatctgccggttgatgttgccgcggccaaagcggaaaaaccgagtctgcccctgaaaaaggaaaatagtaccagtaataccagcgatcaggagatcctgaacaagatccaggaaagcctgaaaaatgccaagaaaccgatcgtgattactggccatgagattattagtttcggtctggagaaaaccgtgacccaattcattagcaaaaccaaactgcccattaccaccctgaacttcggtaaaagcagcgttgatgaggcgctgccgagctttctgggcatttacaatggtaccctgagcgaacccaacctgaaagagtttgttgaaagtgcggatttcattctgatgctgggtgttaaactgaccgatagtagtaccggcgccttcactcatcatctgaatgaaaataagatgatcagtctgaacattgatgaaggcaagattttcaacgaacgtatccaaaactttgattttgagagtctgatcagtagtctgttggacctgagcgagattgaatacaagggcaagtacattgacaagaagcaagaagatttcgttccgagtaatgccctgttgagtcaagatcgcctgtggcaagcggtggaaaatctgacccaaagcaacgaaaccattgtggccgaacagggcaccagctttttcggtgccagtagtatttttctgaaaagtaagagccacttcatcggtcaaccgctgtggggcagcattggctatacttttccggccgccctgggcagccaaatcgccgataaagaaagccgccatctgttgtttattggtgatggcagcctgcaactgaccgttcaagagctgggcctggccattcgtgagaagattaatccgatttgtttcattatcaacaacgatggctatactgttgaacgtgagattcatggtccgaaccagagttataatgacattccgatgtggaactacagcaagctgccggaaagcttcggcgccaccgaagatcgcgtggtgagtaagattgtgcgtactgagaacgagttcgttagtgtgatgaaagaggcgcaagccgatccgaatcgtatgtattggattgagctgattctggcgaaagagggtgcgccgaaggttctgaaaaagatgggtaaactgtttgcggagcagaacaaaagttaataatactagagaaagaggagaaatactagatgtctattccagaaactcaaaaagccattatcttctacgaatccaacggcaagttggagcataaggatatcccagttccaaagccaaagcccaacgaattgttaatcaacgtcaagtactctggtgtctgccacaccgatttgcacgcttggcatggtgactggccattgccaactaagttaccattagttggtggtcacgaaggtgccggtgtcgttgtcggcatgggtgaaaacgttaagggctggaagatcggtgactacgccggtatcaaatggttgaacggttcttgtatggcctgtgaatactgtgaattgggtaacgaatccaactgtcctcacgctgacttgtctggttacacccacgacggttctttccaagaatacgctaccgctgacgctgttcaagccgctcacattcctcaaggtactgacttggctgaagtcgcgccaatcttgtgtgctggtatcaccgtatacaaggctttgaagtctgccaacttgagagcaggccactgggcggccatttctggtgctgctggtggtctaggttctttggctgttcaatatgctaaggcgatgggttacagagtcttaggtattgatggtggtccaggaaaggaagaattgtttacctcgctcggtggtgaagtattcatcgacttcaccaaagagaaggacattgttagcgcagtcgttaaggctaccaacggcggtgcccacggtatcatcaatgtttccgtttccgaagccgctatcgaagcttctaccagatactgtagggcgaacggtactgttgtcttggttggtttgccagccggtgcaaagtgctcctctgatgtcttcaaccacgttgtcaagtctatctccattgtcggctcttacgtggggaacagagctgataccagagaagccttagatttctttgccagaggtctagtcaagtctccaataaaggtagttggcttatccagtttaccagaaatttacgaaaagatggagaagggccaaattgctggtagatacgttgttgacacttctaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K273005_sequence
1
atgtctattccagaaactcaaaaagccattatcttctacgaatccaacggcaagttggagcataaggatatcccagttccaaagccaaagcccaacgaattgttaatcaacgtcaagtactctggtgtctgccacaccgatttgcacgcttggcatggtgactggccattgccaactaagttaccattagttggtggtcacgaaggtgccggtgtcgttgtcggcatgggtgaaaacgttaagggctggaagatcggtgactacgccggtatcaaatggttgaacggttcttgtatggcctgtgaatactgtgaattgggtaacgaatccaactgtcctcacgctgacttgtctggttacacccacgacggttctttccaagaatacgctaccgctgacgctgttcaagccgctcacattcctcaaggtactgacttggctgaagtcgcgccaatcttgtgtgctggtatcaccgtatacaaggctttgaagtctgccaacttgagagcaggccactgggcggccatttctggtgctgctggtggtctaggttctttggctgttcaatatgctaaggcgatgggttacagagtcttaggtattgatggtggtccaggaaaggaagaattgtttacctcgctcggtggtgaagtattcatcgacttcaccaaagagaaggacattgttagcgcagtcgttaaggctaccaacggcggtgcccacggtatcatcaatgtttccgtttccgaagccgctatcgaagcttctaccagatactgtagggcgaacggtactgttgtcttggttggtttgccagccggtgcaaagtgctcctctgatgtcttcaaccacgttgtcaagtctatctccattgtcggctcttacgtggggaacagagctgataccagagaagccttagatttctttgccagaggtctagtcaagtctccaataaaggtagttggcttatccagtttaccagaaatttacgaaaagatggagaagggccaaattgctggtagatacgttgttgacacttctaaataataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z