LOCUS       BBa_K273014             2941 bp    DNA     linear       08-May-2024
DEFINITION  RBS - pdc - RBS - adh2(Y) - Ter
ACCESSION   BBa_K273014
VERSION     BBa_K273014.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=startcodon
     misc_feature    1723..1728
                     /label=stopcodon (double)
     CDS             19..1722
                     /label=coding sequence
     CDS             19..1728
                     /label=BBa_K273000
     misc_feature    1741..1744
                     /label=conserved
     RBS             1737..1748
                     /label=BBa_B0034
     misc_feature    1755..1757
                     /label=startcodon
     CDS             1755..2798
                     /label=cds
     misc_feature    2799..2804
                     /label=stopcodon
     CDS             1755..2804
                     /label=BBa_K273005
     stem_loop       2824..2867
                     /label=stem_loop
     misc_feature    2813..2892
                     /label=BBa_B0010
     terminator      2813..2892
                     /label=BBa_B0010
     stem_loop       2908..2927
                     /label=T7 TE
     misc_feature    2934..2934
                     /label=stop
     polyA_site      2928..2941
                     /label=polya
     misc_feature    2901..2941
                     /label=BBa_B0012
     terminator      2901..2941
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gagttatact gtcggtacct atttagcgga gcggcttgtc
       61 cagattggtc tcaagcatca cttcgcagtc gcgggcgact acaacctcgt ccttcttgac
      121 aacctgcttt tgaacaaaaa catggagcag gtttattgct gtaacgaact gaactgcggt
      181 ttcagtgcag aaggttatgc tcgtgccaaa ggcgcagcag cagccgtcgt tacctacagc
      241 gtcggtgcgc tttccgcatt tgatgctatc ggtggcgcct atgcagaaaa ccttccggtt
      301 atcctgatct ccggtgctcc gaacaacaat gatcacgctg ctggtcacgt gttgcatcac
      361 gctcttggca aaaccgacta tcactatcag ttggaaatgg ccaagaacat cacggccgcc
      421 gctgaagcga tttacacccc ggaagaagct ccggctaaaa tcgatcacgt gattaaaact
      481 gctcttcgtg agaagaagcc ggtttatctc gaaatcgctt gcaacattgc ttccatgccc
      541 tgcgccgctc ctggaccggc aagcgcattg ttcaatgacg aagccagcga cgaagcttct
      601 ttgaatgcag cggttgaaga aaccctgaaa ttcatcgcca accgcgacaa agttgccgtc
      661 ctcgtcggca gcaagctgcg cgcagctggt gctgaagaag ctgctgtcaa atttgctgat
      721 gctctcggtg gcgcagttgc taccatggct gctgcaaaaa gcttcttccc agaagaaaac
      781 ccgcattaca tcggcacctc atggggtgaa gtcagctatc cgggcgttga aaagacgatg
      841 aaagaagccg atgcggttat cgctctggct cctgtcttca acgactactc caccactggt
      901 tggacggata ttcctgatcc taagaaactg gttctcgctg aaccgcgttc tgtcgtcgtt
      961 aacggcattc gcttccccag cgtccatctg aaagactatc tgacccgttt ggctcagaaa
     1021 gtttccaaga aaaccggtgc attggacttc ttcaaatccc tcaatgcagg tgaactgaag
     1081 aaagccgctc cggctgatcc gagtgctccg ttggtcaacg cagaaatcgc ccgtcaggtc
     1141 gaagctcttc tgaccccgaa cacgacggtt attgctgaaa ccggtgactc ttggttcaat
     1201 gctcagcgca tgaagctccc gaacggtgct cgcgttgaat atgaaatgca gtggggtcac
     1261 attggttggt ccgttcctgc cgccttcggt tatgccgtcg gtgctccgga acgtcgcaac
     1321 atcctcatgg ttggtgatgg ttccttccag ctgacggctc aggaagtcgc tcagatggtt
     1381 cgcctgaaac tgccggttat catcttcttg atcaataact atggttacac catcgaagtt
     1441 atgatccatg atggtccgta caacaacatc aagaactggg attatgccgg tctgatggaa
     1501 gtgttcaacg gtaacggtgg ttatgacagc ggtgctggta aaggcctgaa ggctaaaacc
     1561 ggtggcgaac tggcagaagc tatcaaggtt gctctggcaa acaccgacgg cccaaccctg
     1621 atcgaatgct tcatcggtcg tgaagactgc actgaagaat tggtcaaatg gggtaagcgc
     1681 gttgctgccg ccaacagccg taagcctgtt aacaagctcc tctaataata ctagagaaag
     1741 aggagaaata ctagatgtct attccagaaa ctcaaaaagc cattatcttc tacgaatcca
     1801 acggcaagtt ggagcataag gatatcccag ttccaaagcc aaagcccaac gaattgttaa
     1861 tcaacgtcaa gtactctggt gtctgccaca ccgatttgca cgcttggcat ggtgactggc
     1921 cattgccaac taagttacca ttagttggtg gtcacgaagg tgccggtgtc gttgtcggca
     1981 tgggtgaaaa cgttaagggc tggaagatcg gtgactacgc cggtatcaaa tggttgaacg
     2041 gttcttgtat ggcctgtgaa tactgtgaat tgggtaacga atccaactgt cctcacgctg
     2101 acttgtctgg ttacacccac gacggttctt tccaagaata cgctaccgct gacgctgttc
     2161 aagccgctca cattcctcaa ggtactgact tggctgaagt cgcgccaatc ttgtgtgctg
     2221 gtatcaccgt atacaaggct ttgaagtctg ccaacttgag agcaggccac tgggcggcca
     2281 tttctggtgc tgctggtggt ctaggttctt tggctgttca atatgctaag gcgatgggtt
     2341 acagagtctt aggtattgat ggtggtccag gaaaggaaga attgtttacc tcgctcggtg
     2401 gtgaagtatt catcgacttc accaaagaga aggacattgt tagcgcagtc gttaaggcta
     2461 ccaacggcgg tgcccacggt atcatcaatg tttccgtttc cgaagccgct atcgaagctt
     2521 ctaccagata ctgtagggcg aacggtactg ttgtcttggt tggtttgcca gccggtgcaa
     2581 agtgctcctc tgatgtcttc aaccacgttg tcaagtctat ctccattgtc ggctcttacg
     2641 tggggaacag agctgatacc agagaagcct tagatttctt tgccagaggt ctagtcaagt
     2701 ctccaataaa ggtagttggc ttatccagtt taccagaaat ttacgaaaag atggagaagg
     2761 gccaaattgc tggtagatac gttgttgaca cttctaaata ataatactag agccaggcat
     2821 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     2881 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     2941 a
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