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BBa_K273015

BBa_K273015 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K273015

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Karl Brune

Date created: 2009-10-16 11:00:00

Date modified: 2015-05-08 01:11:44

PLlac - RBS - pdc - RBS - adh2(Y) - Ter

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Details

Types	DnaRegion
Roles	Composite engineered_region
Sequences	BBa_K273015_sequence (Version 1)

Description

This construct encodes for an ethanol producing pathway. The pathway uses pyruvate as a precursor.

We used this construct for testing and characterization purposes in E. coli.

Notes

Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from S. cerevisiae was used instead of the adh2 from Z. mobilis

Source

Pyruvate decarboxylase (pdc) from Z. Mobilis ATCC 29191
Alcohol Dehydrogenase 2 (adh2 , adhII, adhB) from S. cerevisiae
Assembled by the Uppsala iGEM Team 2009

Components

Access	Instance	Definition
public public public public public public public	BBa_R0011 BBa_B0034 BBa_K273000 BBa_B0034 BBa_K273005 BBa_B0010 BBa_B0012	lacI+pL BBa_B0034 pdc BBa_B0034 adh2 BBa_B0010 BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0011 BBa_B0034 BBa_K273000 BBa_B0034 BBa_K273005 BBa_B0010 BBa_B0012	1,54 64,75 82,1791 1800,1811 1818,2867 2876,2955 2964,3004	lacI+pL BBa_B0034 pdc BBa_B0034 adh2 BBa_B0010 BBa_B0012

Other Properties

igem#experience	None
igem#sampleStatus	Not in stock
igem#status	Unavailable
synbiohub#ownedBy	user/james
synbiohub#ownedBy	user/myers
synbiohub#topLevel	BBa_K273015/1