LOCUS       BBa_K273016             3378 bp    DNA     linear       03-May-2024
DEFINITION  pPetE - RBS - pdc - RBS - adh2(Y) - Ter
ACCESSION   BBa_K273016
VERSION     BBa_K273016.1  
FEATURES             Location/Qualifiers
     promoter        1..429
                     /label=BBa_K273019
     misc_feature    442..445
                     /label=conserved
     RBS             438..449
                     /label=BBa_B0034
     misc_feature    456..458
                     /label=startcodon
     misc_feature    2160..2165
                     /label=stopcodon (double)
     CDS             456..2159
                     /label=coding sequence
     CDS             456..2165
                     /label=BBa_K273000
     misc_feature    2178..2181
                     /label=conserved
     RBS             2174..2185
                     /label=BBa_B0034
     misc_feature    2192..2194
                     /label=startcodon
     CDS             2192..3235
                     /label=cds
     misc_feature    3236..3241
                     /label=stopcodon
     CDS             2192..3241
                     /label=BBa_K273005
     stem_loop       3261..3304
                     /label=stem_loop
     misc_feature    3250..3329
                     /label=BBa_B0010
     terminator      3250..3329
                     /label=BBa_B0010
     stem_loop       3345..3364
                     /label=T7 TE
     misc_feature    3371..3371
                     /label=stop
     polyA_site      3365..3378
                     /label=polya
     misc_feature    3338..3378
                     /label=BBa_B0012
     terminator      3338..3378
                     /label=BBa_B0012
ORIGIN
        1 tcatagcggt tgcccaatct aactcaggga gcgacttcag cccacaaaaa acaccactgg
       61 gcctactggg ctattcccat tatcatctac attgaaggga tagcaagcta atttttatga
      121 cggcgatcgc caaaaacaaa gaaaattcag caattaccgt gggtagcaaa aaatccccat
      181 ctaaagttca gtaaatatag ctagaacaac caagcatttt cggcaaagta ctattcagat
      241 agaacgagaa atgagcttgt tctatccgcc cggggctgag gctgtataat ctacgacggg
      301 ctgtcaaaca ttgtgatacc atgggcagaa gaaaggaaaa acgtccctga tcgccttttt
      361 gggcacggag tagggcgtta ccccggcccg ttcaaccaca agtccctata gatacaatcg
      421 ccaagaagtt actagagaaa gaggagaaat actagatgag ttatactgtc ggtacctatt
      481 tagcggagcg gcttgtccag attggtctca agcatcactt cgcagtcgcg ggcgactaca
      541 acctcgtcct tcttgacaac ctgcttttga acaaaaacat ggagcaggtt tattgctgta
      601 acgaactgaa ctgcggtttc agtgcagaag gttatgctcg tgccaaaggc gcagcagcag
      661 ccgtcgttac ctacagcgtc ggtgcgcttt ccgcatttga tgctatcggt ggcgcctatg
      721 cagaaaacct tccggttatc ctgatctccg gtgctccgaa caacaatgat cacgctgctg
      781 gtcacgtgtt gcatcacgct cttggcaaaa ccgactatca ctatcagttg gaaatggcca
      841 agaacatcac ggccgccgct gaagcgattt acaccccgga agaagctccg gctaaaatcg
      901 atcacgtgat taaaactgct cttcgtgaga agaagccggt ttatctcgaa atcgcttgca
      961 acattgcttc catgccctgc gccgctcctg gaccggcaag cgcattgttc aatgacgaag
     1021 ccagcgacga agcttctttg aatgcagcgg ttgaagaaac cctgaaattc atcgccaacc
     1081 gcgacaaagt tgccgtcctc gtcggcagca agctgcgcgc agctggtgct gaagaagctg
     1141 ctgtcaaatt tgctgatgct ctcggtggcg cagttgctac catggctgct gcaaaaagct
     1201 tcttcccaga agaaaacccg cattacatcg gcacctcatg gggtgaagtc agctatccgg
     1261 gcgttgaaaa gacgatgaaa gaagccgatg cggttatcgc tctggctcct gtcttcaacg
     1321 actactccac cactggttgg acggatattc ctgatcctaa gaaactggtt ctcgctgaac
     1381 cgcgttctgt cgtcgttaac ggcattcgct tccccagcgt ccatctgaaa gactatctga
     1441 cccgtttggc tcagaaagtt tccaagaaaa ccggtgcatt ggacttcttc aaatccctca
     1501 atgcaggtga actgaagaaa gccgctccgg ctgatccgag tgctccgttg gtcaacgcag
     1561 aaatcgcccg tcaggtcgaa gctcttctga ccccgaacac gacggttatt gctgaaaccg
     1621 gtgactcttg gttcaatgct cagcgcatga agctcccgaa cggtgctcgc gttgaatatg
     1681 aaatgcagtg gggtcacatt ggttggtccg ttcctgccgc cttcggttat gccgtcggtg
     1741 ctccggaacg tcgcaacatc ctcatggttg gtgatggttc cttccagctg acggctcagg
     1801 aagtcgctca gatggttcgc ctgaaactgc cggttatcat cttcttgatc aataactatg
     1861 gttacaccat cgaagttatg atccatgatg gtccgtacaa caacatcaag aactgggatt
     1921 atgccggtct gatggaagtg ttcaacggta acggtggtta tgacagcggt gctggtaaag
     1981 gcctgaaggc taaaaccggt ggcgaactgg cagaagctat caaggttgct ctggcaaaca
     2041 ccgacggccc aaccctgatc gaatgcttca tcggtcgtga agactgcact gaagaattgg
     2101 tcaaatgggg taagcgcgtt gctgccgcca acagccgtaa gcctgttaac aagctcctct
     2161 aataatacta gagaaagagg agaaatacta gatgtctatt ccagaaactc aaaaagccat
     2221 tatcttctac gaatccaacg gcaagttgga gcataaggat atcccagttc caaagccaaa
     2281 gcccaacgaa ttgttaatca acgtcaagta ctctggtgtc tgccacaccg atttgcacgc
     2341 ttggcatggt gactggccat tgccaactaa gttaccatta gttggtggtc acgaaggtgc
     2401 cggtgtcgtt gtcggcatgg gtgaaaacgt taagggctgg aagatcggtg actacgccgg
     2461 tatcaaatgg ttgaacggtt cttgtatggc ctgtgaatac tgtgaattgg gtaacgaatc
     2521 caactgtcct cacgctgact tgtctggtta cacccacgac ggttctttcc aagaatacgc
     2581 taccgctgac gctgttcaag ccgctcacat tcctcaaggt actgacttgg ctgaagtcgc
     2641 gccaatcttg tgtgctggta tcaccgtata caaggctttg aagtctgcca acttgagagc
     2701 aggccactgg gcggccattt ctggtgctgc tggtggtcta ggttctttgg ctgttcaata
     2761 tgctaaggcg atgggttaca gagtcttagg tattgatggt ggtccaggaa aggaagaatt
     2821 gtttacctcg ctcggtggtg aagtattcat cgacttcacc aaagagaagg acattgttag
     2881 cgcagtcgtt aaggctacca acggcggtgc ccacggtatc atcaatgttt ccgtttccga
     2941 agccgctatc gaagcttcta ccagatactg tagggcgaac ggtactgttg tcttggttgg
     3001 tttgccagcc ggtgcaaagt gctcctctga tgtcttcaac cacgttgtca agtctatctc
     3061 cattgtcggc tcttacgtgg ggaacagagc tgataccaga gaagccttag atttctttgc
     3121 cagaggtcta gtcaagtctc caataaaggt agttggctta tccagtttac cagaaattta
     3181 cgaaaagatg gagaagggcc aaattgctgg tagatacgtt gttgacactt ctaaataata
     3241 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
     3301 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
     3361 ggcctttctg cgtttata
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