LOCUS       BBa_K283000             1750 bp    DNA     linear       01-Jun-2024
DEFINITION  pTet-rbs-lpp_ompA_eGFP_streptavidin-double Terminators
ACCESSION   BBa_K283000
VERSION     BBa_K283000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..167
                     /label=BBa_J36835
     CDS             168..509
                     /label=BBa_J36837
     CDS             510..1613
                     /label=cds
     CDS             510..1613
                     /label=BBa_K283009
     CDS             81..1613
                     /label=BBa_K283005
     stem_loop       1633..1676
                     /label=stem_loop
     misc_feature    1622..1701
                     /label=BBa_B0010
     terminator      1622..1701
                     /label=BBa_B0010
     stem_loop       1717..1736
                     /label=T7 TE
     misc_feature    1743..1743
                     /label=stop
     polyA_site      1737..1750
                     /label=polya
     misc_feature    1710..1750
                     /label=BBa_B0012
     terminator      1710..1750
                     /label=BBa_B0012
     terminator      1622..1750
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaagcta ctaaactggt actgggcgcg gtaatcctgg
      121 gttctactct gctggcaggt tgctccagca acgctaaaat cgatcagaac ccgtatgttg
      181 gctttgaaat gggttacgac tggttaggtc gtatgccgta caaaggcagc gttgaaaacg
      241 gtgcatacaa agctcagggc gttcaactga ccgctaaact gggttaccca atcactgacg
      301 acctggacat ctacactcgt ctgggtggca tggtatggcg tgcagacact aaatccaacg
      361 tttatggtaa aaaccacgac accggcgttt ctccggtctt cgctggcggt gttgagtacg
      421 cgatcactcc tgaaatcgct acccgtctgg aataccagtg gaccaacaac atcggtgacg
      481 cacacaccat cggcactcgt ccggacaaca tggtgagcaa gggcgaggag ctgttcaccg
      541 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt
      601 ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca
      661 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt
      721 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
      781 aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg
      841 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
      901 tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg
      961 tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca
     1021 acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     1081 acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag
     1141 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca
     1201 ctctcggcat ggacgagctg tacaaggagc tcgctgaagc tggtatcacc ggcacctggt
     1261 acaaccagct gggatccacc ttcatcgtta ccgctggtgc tgacggtgct ctgaccggta
     1321 cctacgaatc cgctgttggt aacgctgaaa gccgctacgt tctgaccggt cgttacgact
     1381 ccgctccggc taccgacggt tccggaaccg ctctgggttg gaccgttgct tggaaaaaca
     1441 actaccgtaa cgctcactcc gctaccacct ggtctggcca gtacgttggt ggtgctgaag
     1501 ctcgtatcaa cacccagtgg ttgttgacct ccggcaccac cgaagccaac gcgtggaaat
     1561 ccaccctggt tggtcacgac accttcacca aagttaaacc gtccgctgct tcctactaga
     1621 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     1681 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     1741 tgcgtttata
//