LOCUS       BBa_K283003              930 bp    DNA     linear       29-May-2024
DEFINITION  pTet-rbs-chez_histag-double terminator
ACCESSION   BBa_K283003
VERSION     BBa_K283003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..725
                     /label=cheZ
     misc_feature    81..775
                     /label=BBa_K094100
     misc_feature    776..793
                     /label=6-His tag
     CDS             81..793
                     /label=BBa_K283008
     stem_loop       813..856
                     /label=stem_loop
     misc_feature    802..881
                     /label=BBa_B0010
     terminator      802..881
                     /label=BBa_B0010
     stem_loop       897..916
                     /label=T7 TE
     misc_feature    923..923
                     /label=stop
     polyA_site      917..930
                     /label=polya
     misc_feature    890..930
                     /label=BBa_B0012
     terminator      890..930
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatgcaac catcaatcaa acctgctgac gagcattcag
      121 ctggcgatat cattgcgcgc atcggcagcc tgacgcgtat gctgcgcgac agtttgcggg
      181 aactggggct ggatcaggcc attgccgaag cggcggaagc catccccgat gcgcgcgatc
      241 gtttgtacta tgttgtgcag atgaccgccc aggctgcgga gcgggcgctg aacagtgttg
      301 aggcgtcaca accgcatcag gatcaaatgg agaaatcagc aaaagcgtta acccaacgtt
      361 gggatgactg gtttgccgat ccgattgacc ttgccgacgc ccgtgaactg gtaacagata
      421 cacgacaatt tctggcagat gtacccgcgc ataccagctt tactaacgcg caactgctgg
      481 aaatcatgat ggcgcaggat tttcaggatc tcaccgggca ggtcattaag cggatgatgg
      541 atgtcattca ggagatcgaa cgccagttgc tgatggtgct gttggaaaac atcccggaac
      601 aggagtcgcg tccaaaacgt gaaaaccaga gtttgcttaa tggacctcag gtcgatacca
      661 gcaaagccgg tgtggtagcc agtcaggatc aggtggacga tttgttggat agtcttggat
      721 tttgagctag caacgcaatg acgtcggaat tgccagctgg ggcgccctct ggtaacatca
      781 tcaccatcac cactactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact
      841 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct
      901 caccttcggg tgggcctttc tgcgtttata
//