LOCUS       BBa_K283015             3177 bp    DNA     linear       01-Jun-2024
DEFINITION  pTet-rbs-invasin(1-625aa)_RFP-terminators
ACCESSION   BBa_K283015
VERSION     BBa_K283015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..3040
                     /label=invasin(1-625)_ RFP
     misc_feature    3049..3128
                     /label=BBa_B0010
     stem_loop       3060..3103
                     /label=stem_loop
     misc_feature    3137..3177
                     /label=BBa_B0012
     stem_loop       3144..3163
                     /label=T7 TE
     polyA_site      3164..3177
                     /label=polya
     misc_feature    3170..3170
                     /label=stop
     misc_feature    81..3177
                     /label=BBa_K283040
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgatggttt tccagccaat cagtgagttt ctcttgataa
      121 ggaatgcggg aatgtctatg tattttaata aaataatttc atttaatatt atttcacgaa
      181 tagttatttg tatctttttg atatgtggaa tgttcatggc tggggcttca gaaaaatatg
      241 atgctaacgc accgcaacag gtccagcctt attctgtctc ttcatctgca tttgaaaatc
      301 tccatcctaa taatgaaatg gagagttcaa tcaatccctt ttccgcatcg gatacagaaa
      361 gaaatgctgc aataatagat cgcgccaata aggagcagga gactgaagcg gtgaataaga
      421 tgataagcac cggggccagg ttagctgcat caggcagggc atctgatgtt gctcactcaa
      481 tggtgggcga tgcggttaat caagaaatca aacagtggtt aaatcgattc ggtacggctc
      541 aagttaatct gaattttgac aaaaattttt cgctaaaaga aagctctctt gattggctgg
      601 ctccttggta tgactctgct tcattcctct tttttagtca gttaggtatt cgcaataaag
      661 acagccgcaa cacacttaac cttggcgtcg ggatacgtac attggagaac ggttggctgt
      721 acggacttaa tactttttat gataatgatt tgaccggcca caaccaccgt atcggtcttg
      781 gtgccgaggc ctggaccgat tatttacagt tggctgccaa tgggtatttt cgcctcaatg
      841 gatggcactc gtcgcgtgat ttctccgact ataaagagcg cccagccact gggggggatt
      901 tgcgcgcgaa tgcttattta cctgcactcc cacaactggg ggggaagttg atgtatgagc
      961 aatacaccgg tgagcgtgtt gctttatttg gtaaagataa tctgcaacgc aacccttatg
     1021 ccgtgactgc cgggatcaat tacacccccg tgcctctact cactgtcggg gtagatcagc
     1081 gtatggggaa aagcagtaag catgaaacac agtggaacct ccaaatgaac tatcgcctgg
     1141 gcgagagttt tcagtcgcaa cttagccctt cagcggtggc aggaacacgt ctactggcgg
     1201 agagccgcta taaccttgtc gatcgtaaca ataatatcgt gttggagtat cagaaacagc
     1261 aggtggttaa actgacatta tcgccagcaa ctatctccgg cctgccgggt caggtttatc
     1321 aggtgaacgc acaagtacaa ggggcatctg ctgtaaggga aattgtctgg agtgatgccg
     1381 aactgattgc cgctggcggc acattaacac cactgagtac cacacaattc aacttggttt
     1441 taccgcctta taaacgcaca gcacaagtga gtcgggtaac ggacgacctg acagccaact
     1501 tttattcgct tagtgcgctc gcggttgatc accaaggaaa ccgatctaac tcattcacat
     1561 tgagcgtcac cgttcagcag cctcagttga cattaacggc ggccgtcatt ggtgatggcg
     1621 caccggctaa tgggaaaact gcaatcaccg ttgagttcac cgttgctgat tttgagggga
     1681 aacccttagc cgggcaggag gtggtgataa ccaccaataa tggtgcgcta ccgaataaaa
     1741 tcacggaaaa gacagatgca aatggcgtcg cgcgcattgc attaaccaat acgacagatg
     1801 gcgtgacggt agtcacagca gaagtggagg ggcaacggca aagtgttgat acccactttg
     1861 ttaagggtac tatcgcggcg gataaatcca ctctggctgc ggtaccgaca tctatcatcg
     1921 ctgatggtct aatggcttca accatcacgt tggagttgaa ggatacctat ggggacccgc
     1981 aggctggcgc gaatgtggct tttgacacaa ccttaggcaa tatgggcgtt atcacggatc
     2041 acaatgacgg cacttatagc gcaccattga ccagtaccac gttgggggta gcaacagtaa
     2101 cggtgaaagt ggatggggct gcgttcagtg tgccgagtgt gacggttaat ttcacggcag
     2161 atcctattcc agatgctggc cgctccagtt tcaccgtctc cacaccggat atcttggctg
     2221 atggcacgat gagttccaca ttatcctttg tccctgtcga taagaatggc cattttatca
     2281 gtgggatgca gggcttgagt tttactcaaa acggtgtgcc ggtgagtatt agccccatta
     2341 ccgagcagcc agatagctat accgcgacgg tggttgggaa tagtgtcggt gatgtcacaa
     2401 tcacgccgca ggttgatacc ctgatactga gtacattgca gaaaaaaata tccctattcc
     2461 cggtacctac gctgaccggt attctggtta acgggcaaaa tttcgctacg gataaagggt
     2521 tcccgaaaac gatctttaaa aacgccacat tccagttaca gatggataac gatgttgcta
     2581 ataatactca gtatgagtgg tcgtcgtcat tcacacccaa tgtatcggtt aacgatcagg
     2641 gtcaggtgac gattacctac caaacctata gcgaagtggc tgtgacggcg aaaagtaaaa
     2701 aattcccaag ttattcggtg agttatcggt tctacccaaa tcggtggata tacgatggcg
     2761 gcagatcgct ggtatccagt ctcgaggcca gcagacaatg ccaaggttca gatatgtctg
     2821 cggttcttga atcctcacgt gcaaccaacg gaacgcgtgc gcctgacggg acattgtggg
     2881 gcgagtgggg gagcttgacc gcgtatagtt ctgattggca atctggtgaa tattgggtca
     2941 aaaagaccag cacggatttt gaaaccatga atatggacac aggcgcactg caaccagggc
     3001 ctgcatactt ggcgttcccg ctctgtgcgc tgtcaatata tactagagcc aggcatcaaa
     3061 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     3121 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//