LOCUS       BBa_K284020             1782 bp    DNA     linear       04-May-2024
DEFINITION  EYFP regulated by lactate responsive promoter (JEN1)
ACCESSION   BBa_K284020
VERSION     BBa_K284020.1  
FEATURES             Location/Qualifiers
     primer_bind     1..19
                     /label=primer_binding
     promoter        1..998
                     /label=JEN1 Promoter
     primer_bind     976..998
                     /label=primer_binding
     misc_feature    862..873
                     /label=TATA-Like
     promoter        1..998
                     /label=BBa_K284002
     CDS             1005..1751
                     /label=Venus YFP
     misc_feature    1005..1019
                     /label=MATSG linker
     misc_feature    1737..1751
                     /label=GSGTA linker
     misc_feature    1758..1782
                     /label=Help:Barcodes
     misc_feature    1005..1782
                     /label=BBa_E2030
ORIGIN
        1 tgattggcaa acctgttgtc ccgggaaacg aacaccacat atattatccg gtgaagcgtg
       61 gagtgaagcg tttcgcagtg gcttttccac cacccccacg ctccaacaac ctacactata
      121 cgtgccgaaa cggctaaata ttagccgttc tccacacact gcccgcagaa ccctggaagg
      181 aaaagaaaag attttccctc ctcgaagcgt ggaacaacaa tctctcttct cttctctcca
      241 gtacttctga ccatcaaaca actcgataaa ccacacatcc tgcgattctc ggagtctggg
      301 gtgtgtttcc tcctctggag caacggactt gcctaattac agcgaatttc cggagatagg
      361 ataatggcaa tacccgtttg cttctaccac atgctgcatc cgccaacctt acgaagggtt
      421 cttcatgccc ttttgcctcc tctatgcgac ttttaagcta cttttagata tggggcattt
      481 gcttattgtc cctgcgaatt ggcgactgga taggcggata tatccaaatt ccgtaactga
      541 gatgatgata ctctctcaaa cacttacctt aaacgtcatg cttctgtgcc ggttactttg
      601 gctattccat ctggtttctt tccttctttc cttccttcct gaaacgctct ccagttcttc
      661 tctgttgagt gacgaggggc gagtattttc cagtgttccg gttatccagg tagtggtttt
      721 gatttagtta ttgtgatgat ctgtgaaact tttttttttt tttttcacta acctcatctc
      781 cggtaccttt ttactatctc tcactaaaat aactctaatt cagtatgagc ccgctggatg
      841 taccacacct gcgtttcttg atatataaaa gtaactggtt tggcgtcttt ttttcgacaa
      901 tcaacgttct tccaatcttg ttgtggttgt gtcttattat tattgtaatg tatgtcttgt
      961 atgatctgga gtaaccaaaa ccaaaacaga tttcaaccta ctagatggca actagcggca
     1021 tggttagtaa aggagaagaa cttttcactg gagttgtccc aattttagtt gaactagatg
     1081 gcgacgtgaa cggtcataag ttcagtgtct ccggcgaagg tgagggtgat gcaacgtacg
     1141 gtaagttaac tttgaagtta atatgtacaa ccggcaagct gcctgttccc tggcctaccc
     1201 tggtgacaac gttaggttat gggttgatgt gctttgctag atacccagat cacatgaaaa
     1261 ggcatgactt ctttaaatct gcaatgccag aaggttacgt ccaagaacgt actattttct
     1321 ttaaagatga cggtaattat aaaactaggg ctgaagttaa attcgaaggt gacacacttg
     1381 taaatcgaat agagttaaag gggattgatt tcaaagagga tggtaatatt ctaggccata
     1441 aacttgaata taactataat tcacacaacg tttacattac cgccgacaag cagaagaatg
     1501 gaatcaaagc caattttaag attagacaca atattgagga tggtggagta cagcttgctg
     1561 atcattacca acaaaatacc ccgatcggtg atggaccagt tttgctaccc gataaccatt
     1621 atctgtccta tcaaagcaaa ttgtcaaaag atcctaacga aaaaagagac cacatggtac
     1681 tcttggaatt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaaggtt
     1741 ctggtaccgc ataataaccc tgatagtgct agtgtagatc cc
//