LOCUS       BBa_K284080             1166 bp    DNA     linear       17-May-2024
DEFINITION  Upstream construction flanking FSTK gene
ACCESSION   BBa_K284080
VERSION     BBa_K284080.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..77
                     /label=RBS-1\Strong
     RBS             70..74
                     /label=RBS
     misc_feature    63..77
                     /label=BBa_B0030
     RBS             63..77
                     /label=BBa_B0030
     misc_feature    86..198
                     /label=BBa_K284033
     stem_loop       218..261
                     /label=stem_loop
     misc_feature    207..286
                     /label=BBa_B0010
     terminator      207..286
                     /label=BBa_B0010
     stem_loop       302..321
                     /label=T7 TE
     misc_feature    328..328
                     /label=stop
     polyA_site      322..335
                     /label=polya
     misc_feature    295..335
                     /label=BBa_B0012
     terminator      295..335
                     /label=BBa_B0012
     misc_feature    344..362
                     /label=TetR 1
     misc_feature    344..397
                     /label=BBa_R0040
     promoter        363..368
                     /label=-35
     misc_feature    369..387
                     /label=TetR 2
     promoter        386..391
                     /label=-10
     promoter        344..397
                     /label=BBa_R0040
     RBS             406..420
                     /label=RBS-1\Strong
     RBS             413..417
                     /label=RBS
     misc_feature    406..420
                     /label=BBa_B0030
     RBS             406..420
                     /label=BBa_B0030
     misc_feature    427..429
                     /label=start
     misc_feature    985..987
                     /label=stop
     CDS             427..987
                     /label=cds
     CDS             427..987
                     /label=BBa_K284032
     stem_loop       1007..1050
                     /label=stem_loop
     misc_feature    996..1075
                     /label=BBa_B0010
     terminator      996..1075
                     /label=BBa_B0010
     stem_loop       1091..1110
                     /label=T7 TE
     misc_feature    1117..1117
                     /label=stop
     polyA_site      1111..1124
                     /label=polya
     misc_feature    1084..1124
                     /label=BBa_B0012
     terminator      1084..1124
                     /label=BBa_B0012
     misc_feature    1133..1166
                     /label=BBa_I718016
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agattaaaga ggagaaatac tagagtgacg agcatgtttt tgttgaatac gatccatcgg
      121 atacatagga acctcctcac aaaggattct atggacagtc gatgcaggga gttcacgtct
      181 ccctgcatcg gcgattttta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      241 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      301 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagtccctat cagtgataga
      361 gattgacatc cctatcagtg atagagatac tgagcactac tagagattaa agaggagaaa
      421 tactagatga cagagcagaa acgaccggta ctgacactga aacggaaaac ggaaggggaa
      481 acaccgaccc ggagccggaa aaccatcatc aatgtcacca cgccaccaaa atggaaggtg
      541 aaaaagcaga aactggcgga gaaggctgcc cgggaagcag agctgacagc aaaaaaagcg
      601 caggccagac aggcgctgtc catttatctg aacctgccct cgctggatga ggccgtgaac
      661 accctgaaac cctggtggcc gggattattt gacggtgaca caccccgact tctggcctgc
      721 ggtatccggg acgtgttact ggaagacgtg gcgcagcgga atatcccgct ctcgcataaa
      781 aaactgcgca gggcgctgaa ggccatcacc cgttcagaaa gctatctgtg tgccatgaaa
      841 gccggtgcct gccggtatga cacggaaggg tatgtgacgg agcatatttc tcaggaggaa
      901 gaagtgtatg cggcagagcg tctggataaa atccgccgcc agaaccggat aaaggcagaa
      961 cttcaggccg tgcttgatga acaataatac tagagccagg catcaaataa aacgaaaggc
     1021 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1081 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agataacttg
     1141 gtatagcata cattatacga acggta
//